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Histological localization of oil palm fruit lipase

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Journal of the American Oil Chemists’ Society

Abstract

Development of free fatty acids (FFA) and subsequent deterioration of palm oil quality can be attributed to lipase, an active hydrolytic enzyme present in the mesocarp of oil palm fruit. A histochemical method was used to localize this enzyme in the mesocarp. FFA released from endogenous glycerides were precipitated as lead salt. The deposits were located on the membranes of oil bodies. Enzymatic activity in the mesocarp was also studied under different conditions of fruit storage: ambient temperature, freezing, etc. The enzyme was active after freezing but less active under ambient conditions of fruit storage. Investigations on fruit development indicated that the enzyme activity started appearing between 16–20 weeks after anthesis (WAA) which synchronized with the beginnning of lipid formation. These histochemical studies were supported wity biochemical data on FFA.

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References

  1. Oo, K.C.,OLeagineux 36:613 (1981).

    CAS  Google Scholar 

  2. Abigor, D.R., F. Opute, A.R. Opoku and A.U. Osagie,J. Sci. Food Agric. 36:599 (1985).

    Article  CAS  Google Scholar 

  3. Gomori, G.,Microscopic Histochemistry, University of Chicago Press, Chicago, IL, 1952, p. 167.

    Google Scholar 

  4. Gomori, G.,J. Histochem. Cytochem. 1:469 (1953).

    CAS  Google Scholar 

  5. Arumughan, C., A. Sundaresan and A.D. Damodaran,J. Food Sci. Technol. 26:277 (1989).

    Google Scholar 

  6. Ory, R.L., L.Y. Yatsu and H.W. Kircher,Arch. Biochem. Biophys. 123:255 (1968).

    Article  CAS  Google Scholar 

  7. Ory, R.L.,Lipids 4:177 (1968).

    Article  Google Scholar 

  8. Bayliss, O.B. and C.M.W. Adams,Histochem. J. 4:505 (1972).

    Article  CAS  Google Scholar 

  9. Foslch, J., M. Lees and G.H.S. Stanley,J. Biol. Chem. 226:497 (1957).

    Google Scholar 

  10. Pearse, A.G.E.,Histochemistry—Theoretical and Applied, Livingstone, New York, NY, 1980, pp. 762–801.

    Google Scholar 

  11. Gahan, P.B.,Plant Histochemistry and Cytochemistry, Academic Press, New York, NY, 1984, pp. 100–111.

    Google Scholar 

  12. Gurr, M.I., J. Blades, R.S. Appleby, C.G. Smith, M.P. Ribinson and B.W. Nicholas,Eur. J. Biochem. 43:281 (1974).

    Article  CAS  Google Scholar 

  13. Jacks, T.J., L.Y. Yatsu and A.M. Altschu,Plant Physiol. 42:585 (1967).

    Article  CAS  Google Scholar 

  14. Wanner, G. and R.R. Theimer,Planta 140:163 (1978).

    Article  Google Scholar 

  15. Bafor, M.E. and A.U. Osagie,J. Sci. Food Agric. 45:325 (1988).

    Article  CAS  Google Scholar 

  16. Oo, K.C., K.B. Lee and A.S.H. Ong,Phytochemistry 25:405 (1986).

    Article  CAS  Google Scholar 

Download references

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Mohankumar, C., Arumughan, C. & Kaleysa raj, R. Histological localization of oil palm fruit lipase. J Am Oil Chem Soc 67, 665–669 (1990). https://doi.org/10.1007/BF02540419

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  • DOI: https://doi.org/10.1007/BF02540419

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