Abstract
Rats were fed lard-enriched (17%) or corn oil-enriched (17%) diets and were compared with rats fed a low fat (4.5%) diet. Cardiac protein, DNA, phospholipid (PL) and fatty acid (FA) compositions were analyzed. Neutral phospholipase A, lysophospholipase and creatine kinase activities in the membrane and cytosolic compartments were also investigated.
No significant modification of cardiac protein, DNA nor PL was observed among the three groups. Some alterations appeared in the FA composition. A lard-enriched diet induced a significant increase of 22∶5n−3 and 22∶6n−3 in heart phosphatidylcholine (PC) and phosphatidylethanolamine (PE), whereas a linoleic acid-rich diet induced a specific increase of 22∶4n−6 and 22∶5n−6 in these two major PL.
Compared to rats fed the low fat diet, membrane-associated phospholipase A activity, measured by endogenous hydrolysis of membrane PC and PE, showed a significant increase (+45%) for both PL in rats fed corn oil. However, the activity of membrane-associated phospholipases, measured with exogenous [1-14C]dioleoyl PC, was not different among the three groups of rats. Cytoplasmic activity was decreased in rats fed corn oil, and lysophospholipase and creatine phosphate kinase activities were not significantly affected by diet.
FA modification of the long chain n−6 FA induced by corn oil may be responsible for the observed increase in phospholipase activity. Physiological implications are suggested in terms of membrane degradation and prostaglandin production.
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Spritz, N., and Mishkel, A. (1969)J. Clin Invest. 48, 78–86.
Shepherd, J., Packard, J.C., Grundy, S.M., Yeshurun, D., Gotto, A.M. Jr., and Taunton, O.D. (1980)J. Lipid Res. 21, 91–99.
Vega, G.L., Groszek, E., Wolf, R., and Grundy, S.M. (1982)J. Lipid Res. 23, 811–822.
Kramer, J.K.G. (1980)Lipids 15, 651–660.
Charnock, J.S., Dryder, W.F., McMurchie, E.J., Abeywardena, M.Y., and Russel, G.R. (1983)Comp. Biochem. Physiol. 75B, 47–52.
Charnock, J.S., Abeywardena, M.Y., McMurchie, E.J., and Russel, G.R. (1984)Lipids 19, 206–213.
Charnock, J.S., McLennan, P.L., Abeywardena, M.Y., and Russel, G.R. (1985)Ann. Nutr. Metab. 29, 279–288.
Kramer, J.K.G., Farnworth, E.R., and Thompson, B.K. (1985)Lipids 20, 635–644.
Gudbjarnason, S., and Oskarsdottir, G. (1977)Biochim. Biophys. Acta 487, 10–15.
Swanson, J.E., and Kinsella, J.E. (1986)J. Nutr. 116, 514–523.
Charnock, J.S., Abeywardema, M.Y., and McLennan, P.L. (1986)Ann. Nutr. Metab. 30, 393–406.
Spector, A.A., and Yorek, M.A. (1985)J. Lipid Res. 26, 1015–1035.
Stubbs, C.D., and Smith, A.D. (1984)Biochim. Biophys. Acta 779, 89–137.
Weglicki, W.B., Waite, M., Sisson, P., and Shohet, S.B. (1971)Biochim. Biophy. Acta 231, 512–519.
Palmer, J.W., Schmid, P.C., Pfeiffer, D.R., and Schmid, H.H.O. (1981)Arch. Biochem. Biophys. 211, 674–682.
Franson, R.F., Waite, M., and Weglicki, W.B. (1972)Biochemistry 11, 472–476.
Nalbone, G., and Hostetler, K.Y. (1985)J. Lipid Res. 26, 104–114.
Tam, S.W., Man, R.Y.K., and Choy, P.C. (1984)Can. J. Biochem. Cell. Biol. 62, 1269–1274.
Nalbone, G., Hostetler, K.Y., Leonardi, J., Trotz, M., and Lafont, H. (1986)Biochim. Biophys. Acta 877, 88–95.
Wolf, R.A., and Gross, R.W. (1985)J. Biol. Chem. 260, 7295–7303.
Chien, K.R., Reeves, J.P., Buja, M.L., Bonte, F., Parkey, R.W., and Willerson, J.T. (1981)Circ. Res. 48, 711–719.
Man, R.Y.K., Slater, T.L., Pelletier, M.P., and Choy, P.C. (1983)Lipids 18, 677–681.
Weglicki, W. (1980) inDegradative Processes in Heart and Skeletal Muscle (Wildenthal, K., ed.) pp. 377–388, Elsevier/North-Holland Biomedical Press, Amsterdam.
Hostetler, K.Y. (1984) inPhospholipids and Cellular Regulation (Kua, J.F., ed.) Vol. I, pp 182–206, CRC Press, Boca Raton, FL.
Man, R.Y.K., Wong, T., and Choy, P.C. (1983)Life Sci. 32, 1325–1330.
Jimena Abendano, J., and Zahler, P. (1979)Biochim. Biophys. Acta 573, 266–275.
Hanson, V.L., Park, J.Y., Osborn, T.W., and Kiral, R.M. (1981)J. Chromatogr. 205, 393–400.
Amic, J., Lairon, D., and Hauton, J.C. (1972)Clin. Chim. Acta 40, 107–114.
Termine, E., Leonardi, J., Lafont, H., and Nalbone, G.Biochimie, in press.
Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.
Szasz, G., Gruber, W., and Bernt, E. (1976)Clin. Chem. 22, 650–656.
Deeg, R., and Ziegenhorm, J. (1982)J. Clin. Chem. 28, 1574.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.
Volkin, E., and Cohn, W.E. (1954)Biochem. Anal. 1, 287–303.
Roblee, N.M., and Clandinin, M.T. (1984)J. Nutr. 114, 263–269.
Awad, A.B., and Chattopadhyay, J.P. (1983)J. Nutr. 113, 1878–1884.
Brenner, R.R. (1966)Prog. Lipid Res. 20, 41–47.
Rocquelin, G., Yoyo, N., and Ducruet, J.M. (1986)Reprod. Nutr. Develop. 26, (1A), 97–112.
Spector, A.A., Kaduce, T.L., and Dane, R.W. (1980)J. Lipid Res. 21, 169–179.
Shukla, S.D. and Hanahan, D.J. (1982)J. Biol. Chem. 257, 2908–2911.
Kannagi, R., Yamagata, Y., and Murachi, T. (1985)Adv. Prostaglandins Thromboxanes Leukotr. 15, 125–127.
ten Hoor, F., de Deckere, F.A., Haddeman, E., Hornstra, G., and Quat, J.P. (1980)Adv. Prostaglandin Thromboxane Res. 8, 1771–1781.
Hoffman, P., Mentz, P., Blass, K.E., and Forster, W. (1982)J. Cardiovasc. Pharmacol. 4, 714–720.
Smirnov, I.E., Fatkhullin, I.A., and Markov, KhM. (1983)Vopr Pitan 3, 58–62.
Shrör, K. (1981)Basic Res. Cardiol. 76, 239–249.
ten Hoor, F. (1980)Nutr. Metab. 24, 162–180.
Hoffmann, P., Taube, C., Heinroth-Hoffmann, I., Fahr, A., Beitz, J., Forster, W., Poleskuk, W.S., and Markov, C.M. (1985)Arch. Int. Pharmacodyn. Ther. 276, 222–235.
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Leonardi, J., Termine, E., Morand, F. et al. Effect of dietary lipids on the lipid composition and phospholipid deacylating enzyme activities of rat heart. Lipids 22, 517–522 (1987). https://doi.org/10.1007/BF02540368
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DOI: https://doi.org/10.1007/BF02540368