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Lipase catalyzed alcoholysis of sunflower oil

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Journal of the American Oil Chemists' Society

Abstract

Lipase-catalyzed alcoholysis of sunflower oil under anhydrous conditions was examined. Lipases fromPseudomonas fluorescens and 2 immobilized enzymes fromMucor miehei and aCandida sp. gave sufficient conversion with petroleum ether as the solvent, even when methanol and ethanol were used. The overall content of tri-, di- and monoglycerides, as well as the corresponding alkyl esters, was measured. BecausePseudomonas lipase led to almost quantitative esterification, further studies were carried out with that enzyme varying the amounts of enzyme or the alcohols. Acceptable conversions were achieved even without solvent. Reaction rates of alcoholysis with 5 homologous alcohols, with or without the addition of water, were measured, and in all cases the reaction rates increased with higher chain length of the alcohol. In the case of methanol the highest rate was obtained without any addition of water, but a significantly higher rate was observed with 96% ethanol as opposed to absolute ethanol. The main advantages of lipasecatalyzed, nonaqueous alcoholysis as compared to classical procedures are the mild reaction conditions, the isolation of glycerin without further purification and without the formation of chemical waste, and the ability of lipases to catalyze the esterification of free fatty acids.

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References

  1. Peterson, C.L.,Transactions of the ASAE 29:1413 (1986).

    Google Scholar 

  2. Mittelbach, M., M. Wörgetter, J. Pernkopf and H. Junek,Energy Agric. 2:369 (1983).

    Article  CAS  Google Scholar 

  3. Mittelbach, M.,Öst. Chem. Ztg. 90:147 (1989).

    CAS  Google Scholar 

  4. Koritala, S., C.W. Hesseltine, E.H. Pryde and T.L. Mounts,J. Am. Oil Chem. Soc. 64:509 (1987).

    CAS  Google Scholar 

  5. Macrae, A.R.,Ibid. 60:243A (1983).

    Google Scholar 

  6. Posorske, L.H.,Ibid. 61:1758 (1984).

    CAS  Google Scholar 

  7. Lazar, G.,Fette Seifen Anstrichm. 87:394 (1985).

    Article  CAS  Google Scholar 

  8. Choo, Y.M. and S.H. Ong,Brit. Pat. Appl. 2188057 (1986);Chem. Abstr. 108:23681k (1988).

  9. Zaks, A. and A.M. Klibanov,Science 224:1249 (1984).

    Article  CAS  Google Scholar 

  10. Zaks, A. and A.M. Klibanov,Proc. Natl. Acad. Sci. 82:3192 (1985).

    Article  CAS  Google Scholar 

  11. Trathnigg, B. and M. Mittelbach,J. Liquid Chromatogr. (1990), in press.

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Mittelbach, M. Lipase catalyzed alcoholysis of sunflower oil. J Am Oil Chem Soc 67, 168–170 (1990). https://doi.org/10.1007/BF02539619

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  • DOI: https://doi.org/10.1007/BF02539619

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