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Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria

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Abstract

Seven soil samples and seven groundwater samples from a site contaminated with fuel-oil were investigated using several chemical and microbiological techniques. In soil samples, 500 to 7,500 mg/kg of total hydrocarbons were found. These samples contained no n-alkanes but iso- and branched chain alkanes. No polychlorinated biphenyls could be detected. Microbiological investigations included estimations of total cell counts, viable cell counts on different media, and numbers of methylotrophic, denitrifying, sulphate reducing, anaerobic (with the exception of methanogenic organisms), and hydrocarbon degrading bacteria. Viable and hydrocarbon degrading bacteria were found in all samples. A total of 1,366 pure cultures was characterized morphologically and physiologically and identified by numerical identification using a data base of more than 4,000 reference strains. Groundwater samples were dominated by gram-negative bacteria of the generaPseudomonas, Comamonas, Alcaligenes, andAcinetobacter, which were also found in soil samples. In addition, more grampositive bacteria belonging to the generaArthrobacter, Nocardia, andBacillus could be isolated from soil samples.

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  1. Fachgebiet Hygiene der Technischen Universität Berlin, Amrumerstrasse 32, 1000, Berlin 65, Federal Republic of Germany

    Peter Kämpfer, Martin Steiof & Wolfgang Dott

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  1. Peter Kämpfer
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  2. Martin Steiof
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  3. Wolfgang Dott
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Kämpfer, P., Steiof, M. & Dott, W. Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251 (1991). https://doi.org/10.1007/BF02539156

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  • DOI: https://doi.org/10.1007/BF02539156

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