Summary
The Nicolau stain deserves to replace the Gutstein stain as a routine method for smallpox diagnosis; it is simple, effective and fool proof.
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Literature
Stains for smallpox diagnosis:C. E. van Rooyen andA. Rhodes, Virus diseases of Man 1948, p. 296.
S. Nicolau, C. R. Soc. Biol.130, 993, 1939.
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Dekking, F. Nicolau stain for smallpox diagnosis. Antonie van Leeuwenhoek 18, 291–292 (1952). https://doi.org/10.1007/BF02538617
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DOI: https://doi.org/10.1007/BF02538617