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Observations on the lyophilization ofBrucella abortus, strain 19

I. Improvement of the survival rate

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Summary

For the production of lyophilizedBrucella abortus Str. 19 vaccine a suspending medium containing 2% tryptose, 0.5% neutralised ascorbic acid and 0.75% thiourea was developed. In 100 separate batches of vaccine dried in this medium the survival rate was determined after primary drying and after secondary drying and vacuum-sealing of the ampoules.

The average survival rate immediately after primary drying was over 88%; in the finished glass sealed ampoules the average was over 82%. It is possible to obtain these results only when a freshly prepared concentrated solution of the reducing substances is added shortly before freezing to the Str. 19 suspension.

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Authors and Affiliations

  1. State Serum Institute, Rotterdam

    A. F. van der Scheer

Authors
  1. A. F. van der Scheer
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van der Scheer, A.F. Observations on the lyophilization ofBrucella abortus, strain 19. Antonie van Leeuwenhoek 22, 193–204 (1956). https://doi.org/10.1007/BF02538327

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  • DOI: https://doi.org/10.1007/BF02538327

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