Summary
After prolonged cultivation at room temperature, papillae are formed by most strains of staphylococci. This secondary growth starts as micro-colonies developing inside the agar, beneath the primary colony; these micro-colonies grow towards the surface, leading to the formation of papillae.
These micro-colonies are formed by micro-aerophilic variants developing during growth of the primary colony.
Coagulase production and hemolytic activity of cultures derived from tested papillae, are similar to that of the mother strain.
Nudlear structure of the bacteria forming the micro-colonies is similar to that of normal cells of staphylococci.
A search for L forms in the micro-colonies remained negative.
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Joiris, E. Etude de la Croissance Bacterienne Secondaire chez les Staphylocoques. Antonie van Leeuwenhoek 22, 167–176 (1956). https://doi.org/10.1007/BF02538324
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DOI: https://doi.org/10.1007/BF02538324