Abstract
The formation of lysophosphatidylinositol phosphate (lysoPIP) from lysophosphatidylinositol (lysoPI) via kinase activity was studied in microsomal preparations from human platelets. For this purpose, [3H]lysoPI or [3H]phosphatidylinositol ([3H]PI) was prepared and incubated in the presence or absence of ATP, MgCl2 and Triton X-100, and the appearances of radioactivity in [3H]lysoPIP and [3H]phosphatidylinositol phosphate ([3H]PIP), respectively, were monitored using thin layer chromatography. Both lysoPI and PI phosphorylations were completely dependent upon the presence of ATP and MgCl2 in the incubation medium; Triton X-100 addition stimulated both reactions, with the stimulation of PI conversion being considerably greater than that for lysoPI conversion. The present results demonstrate that lysoPI can be converted to lysoPIP by phosphorylation in human platelet microsomes. The potential significance of this enzymatic reaction in stimulated cells is discussed in relation to the generation of inositol-1,4,5-trisphosphate, an important intracellular second messenger.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Michell, R.H., Harwood, J.L., Coleman, R., and Hawthorne, J.N. (1967)Biochim. Biophys. Acta 144, 649–658.
Lefebvre, Y.A., White, D.A., and Hawthorne, J.N. (1976)Can. J. Biochem. 54, 746–753.
Cooper, P.H., and Hawthorne, J.N. (1976)Biochem. J. 160, 97–105.
Quist, E.E., and Barker, R.C. (1983)Arch. Biochem. Biophys. 222, 170–178.
Chung, S.M., Proia, A.D., Klintworth, G.K., Watson, S.P., and Lapetina, E.G. (1985)Biochem. Biophys. Res. Commun. 129, 411–416.
Downes, C.P., and Michell, R.H. (1981)Biochem. J. 198, 133–140.
Smith, C.D., and Wells, W.W. (1984)Arch. Biochem. Biophys. 235, 529–537.
Raval, P.J., and Allan, D. (1985)Biochem. J. 231, 179–183.
Keenan, R.W., and Hokin, L.E. (1964)J. Biol. Chem. 239, 2123–2129.
Baker, R.R., and Thompson, W. (1973)J. Biol. Chem. 248, 7060–7065.
Holub, B.J. (1976)Lipids 11, 1–5.
Palmer, F.B.St.C. (1986)Biochem. Cell Biol. 64, 117–125.
Murase, S., and Okuyama, H. (1985)J. Biol. Chem. 260, 262–265.
Billah, M.M., and Lapetina, E.G. (1982)J. Biol. Chem. 257, 5196–5200.
Rittenhouse, S.E., and Allen, C.L. (1982)J. Clin. Invest. 70, 1216–1224.
Rittenhouse, S.E. (1984)Biochem. J. 222, 103–110.
Irvine, R.F., Hemington, N., and Dawson, R.M.C. (1978)Biochem. J. 176, 475–484.
Bligh, E.G., and Dyer, W.J. (1959)Can. J. Biochem. Physiol. 37, 911–917.
Arvidson, G.A.E. (1968)Eur. J. Biochem. 4, 478–486.
Holub, B.J. (1974)Biochim. Biophys. Acta 369, 111–122.
Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.
Allan, D., and Michell, R.H. (1978)Biochim. Biophys. Acta 508, 277–286.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.
Jergil, B., and Sundler, R. (1983)J. Biol. Chem. 258, 7968–7973.
Sandelius, A.S., and Sommarin, M. (1986)FEBS Lett. 201, 282–286.
Marche, P., Koutouzov, S., and Meyer, P. (1982)Biochim. Biophys. Acta 710, 332–340.
Berridge, M.J., and Irvine, R.F. (1984)Nature 312, 315–321.
Joseph, S.K., Thomas, A.P., Williams, R.J., Irvine, R.F., and Williamson, J.R. (1984)J. Biol. Chem. 259, 3077–3081.
Rittenhouse, S.E., and Sasson, J.P. (1985)J. Biol. Chem. 260, 8657–8660.
Brass, L.F., and Joseph, S.K. (1985)J. Biol. Chem. 260, 15172–15179.
Author information
Authors and Affiliations
About this article
Cite this article
Thomas, L.M., Holub, B.J. The formation of lysophosphatidylinositol phosphate in human platelet microsomes. Lipids 22, 144–147 (1987). https://doi.org/10.1007/BF02537292
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02537292