Abstract
Human keratinocytes (NCTC 2544) in culture were labeled with equal amounts of either14C-arachidonic acid,14C-dihomo-γ-linolenic acid or14C-eicosapentaenoic acid. At various time points, the incubations were stopped and the distribution of the14C-fatty acids was analyzed. All these eicosanoid precursor fatty acids were effectively incorporated into the cellular lipids of the keratinocytes, and the major radiolabeled individual lipid fraction was phosphatidylethanolamine. The distributions of arachidonic acid and dihomo-γ-linolenic acid within cellular lipids were rather the same. However, less eicosapentaenoic acid than either arachidonic acid or dihomo-γ-linolenic acid was incorporated into the phospholipids and, correspondingly, more eicosapentaenoic acid was incorporated into the nonphosphorus lipids. In the phosphatidylinositol + phosphatidylserine fraction, there was significantly less eicosapentaenoic acid than either arachidonic acid or dihomo-γ-linolenic acid. The present study suggests that these eicosanoid precursor fatty acids are effectively incorporated into the human keratinocytes and that the pattern of incorporation and distribution of eicosapentaenoic acid appears to differ slightly from that of either arachidonic acid or dihomo-γ-linolenic acid.
Similar content being viewed by others
References
Corey, E.J., Niwa, H., Falck, J.R., Mioskowski, C., Arai, Y., and Marfat, A. (1980)Prostaglandin Thromboxane Res. 6, 19–25.
Kragballe, K., and Voorhees, J.J. (1983)J. Invest. Dermatol. 81, 293–296.
Ruzicka, T., and Printz, M.P. (1984)Rev. Physiol. Biochem. Pharmacol. 100, 121–160.
Irvine, R.F. (1982)Biochem. J. 204, 3–16.
Leitch, A.G., Lee, T.H., Ringel, E.W., Prickett, J.D., Robinson, D.R., Pyne, S.G., Corey, E.J., Drazen, J.M., Austen, K.F., and Lewis, R.A. (1984)J. Immunol. 132, 2559–2565.
Needleman, P.A., Raz, A., Minkes, M.S., Ferrendelli, J.A., and Sprecher, H. (1979)Proc. Natl. Acad. Sci. USA 76, 944–948.
Lee, T.H., Mencia-Huerta, J.M., Shih, C., Corey, E.J., Lewis, R.A., and Austen, K.F. (1984)J. Biol. Chem. 259, 2383–2389.
DeLeo, V.A., Horlick, H., Hanson, O., Eisinger, M., and Harber, L.C. (1984)J. Invest. Dermatol. 83, 323–326.
Punnonen, K., Puustinen, T., and Jansen, C.T. (1986)Arch. Dermatol. Res. 278, 441–444.
Kondoh, H., Sato, Y., and Kanoh, H. (1985)J. Invest. Dermatol. 85, 64–69.
Perry, V.P., Sanford, K.K., Evans, J.J., Hyatt, G.W., and Earle, W.R. (1957)J. Natl. Cancer Inst. 18, 709–717.
Ree, K., Johnsen, A.S., Rugstad, H.E., Bakka, A., and Hovig, T. (1981)Acta Path. Microbiol. Scand. Sect. A 89, 81–90.
Puustinen, T., and Uotila, P. (1983)Prostaglandins 26, 265–274.
Punnonen, K., Puustinen, T., and Jansen, C.T. (1986)Prostaglandins 31, 263–270.
Morimoto, K., and Kanoh, H. (1978)Biochim. Biophys. Acta 531, 16–24.
Morita, I., Saito, Y., Chang, W.C., and Murota, S. (1983)Lipids 18, 42–49.
Laposata, M., Reich, E.L., and Majerus, P.W. (1985)J. Biol. Chem. 260, 11016–11020.
Baker, R.R., and Thompson, W. (1973)J. Biol. Chem. 248, 7060–7065.
Madison, K.C., Wertz, P.W., Strauss, J.S., and Downing, D.T. (1986)J. Invest. Dermatol. 87, 253–259.
Fischer, S., Schacky, C.v., Siess, W., Strasser, T., and Weber, P.C. (1984)Biochem. Biophys. Res. Commun. 120, 907–918.
Schacky, C.v., Siess, W., Fischer, S., and Weber, P.C. (1985),J. Lipid Res. 26, 457–464.
Weaver, B.J., and Holub, B.J. (1985)Lipids 20, 773–777.
Tou, J.-S. (1984)Lipids 19, 573–577.
Lapetina, E.G., Billah, M.M., and Cuatrecasas, P. (1981)Nature 292, 367–369.
Siess, W., Scherer, B., Böhlig, B., Roth, P., Kurzmann, I., and Weber, P.C. (1980)Lancet i, 441–444.
Spector, A.A., and Yorek, M.A. (1985)J. Lipid Res. 26, 1015–1035.
Terano, T., Salmon, J.A., Higgs, G.A., and Moncada, S. (1986)Biochem. Pharmacol 35, 779–785.
Evans, J.F., Nathaniel, D.J., Zamboni, R.J., and Ford-Hutchinson, A.W. (1985)J. Biol. Chem. 260, 10966–10970.
Kremer, J.M., Michalec, A.V., Lininges, L., Hvyck, C., Bigavoette, J., Timchalk, M.A., Rynes, R.I., and Zieminski, J. (1985)Lancet i, 184–187.
Lee, T.H., Hoover, R.L., Williams, J.D., Sperling, R.I., Ravalese, J. III, Spur, B.W., Robinson, D.R., Corey, E.J., Lewis, R.A., and Austen, K.F. (1985)N. Engl. J. Med. 312, 1217–1223.
Author information
Authors and Affiliations
About this article
Cite this article
Punnonen, K., Puustinen, T. & Jansen, C.T. Time course of incorporation of 20-carbon polyunsaturated fatty acids in a human keratinocyte cell line. Lipids 22, 139–143 (1987). https://doi.org/10.1007/BF02537291
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02537291