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Inhibition of cholesterol and fatty acid biosynthesis in liver enzymes and chicken hepatocytes by polar fractions of garlic

Abstract

Different concentrations of polar fractions, methanol-soluble (MESF), or water-soluble (WASF), of 1–8% equivalent to fresh garlic paste were added to yellow corn-soybean based diets and fed to 5-week-old male broiler chickens for 3 weeks to measure the inhibition of hepatic β-hydroxy-β-methylglutaryl coenzyme A (HMG-CoA) reductase, cholesterol 7α-hydroxylase (7α-hydroxy) and fatty acid synthetase (FAS). Dose-related decreases in the activities of these enzymes were obtained. Decreases in serum total cholesterol and in low density lipoprotein (LDL) levels were also observed. There was no effect on the level of cholesterol in high density lipoprotein (HDL). The most effective dose for these decreases was found 0.54% (MESF) and 1.2% (WASF) equivalent to 6% of the fresh garlic. The inhibition of HMG-CoA reductase and FAS by 25–300 μm of MESF or WASF for 15 min was tested in vitro, in male and female chicken hepatocytes. Inhibitions of activity were dose-dependent and the degree of inhibition increased with duration of incubation (150 μg of MESF or WASF 5 to 60 min). Dietary supplementation of odorless WASF of garlic was found to be very effective in lowering the total and LDL cholesterol levels compared to control chickens.

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Correspondence to Asaf A. Qureshi.

Additional information

Cooperative investigation between the Agricultural Research Service, US Department of Agriculture, and College of Agriculture and Life Sciences, University of Wisconsin, Madison. A preliminary report of this work was presented at the 66th Annual Meeting of Federation of American Societies for Experimental Biology, New Orleans, LA, April 1982, Abs. 41∶554 (1982).

Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the US Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable.

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Qureshi, A.A., Abuirmeileh, N., Din, Z.Z. et al. Inhibition of cholesterol and fatty acid biosynthesis in liver enzymes and chicken hepatocytes by polar fractions of garlic. Lipids 18, 343–348 (1983). https://doi.org/10.1007/BF02537229

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Keywords

  • Microsomal Fraction
  • Mevalonic Acid
  • Fatty Acid Synthetase
  • Perfusion Buffer
  • Female Chicken