, Volume 18, Issue 5, pp 343–348 | Cite as

Inhibition of cholesterol and fatty acid biosynthesis in liver enzymes and chicken hepatocytes by polar fractions of garlic

  • Asaf A. Qureshi
  • Naji Abuirmeileh
  • Zafeer Z. Din
  • Charles E. Elson
  • Warren C. Burger


Different concentrations of polar fractions, methanol-soluble (MESF), or water-soluble (WASF), of 1–8% equivalent to fresh garlic paste were added to yellow corn-soybean based diets and fed to 5-week-old male broiler chickens for 3 weeks to measure the inhibition of hepatic β-hydroxy-β-methylglutaryl coenzyme A (HMG-CoA) reductase, cholesterol 7α-hydroxylase (7α-hydroxy) and fatty acid synthetase (FAS). Dose-related decreases in the activities of these enzymes were obtained. Decreases in serum total cholesterol and in low density lipoprotein (LDL) levels were also observed. There was no effect on the level of cholesterol in high density lipoprotein (HDL). The most effective dose for these decreases was found 0.54% (MESF) and 1.2% (WASF) equivalent to 6% of the fresh garlic. The inhibition of HMG-CoA reductase and FAS by 25–300 μm of MESF or WASF for 15 min was tested in vitro, in male and female chicken hepatocytes. Inhibitions of activity were dose-dependent and the degree of inhibition increased with duration of incubation (150 μg of MESF or WASF 5 to 60 min). Dietary supplementation of odorless WASF of garlic was found to be very effective in lowering the total and LDL cholesterol levels compared to control chickens.


Microsomal Fraction Mevalonic Acid Fatty Acid Synthetase Perfusion Buffer Female Chicken 
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  1. 1.
    Carroll, K. K., and Hamilton, R. M. G. (1975) J. Food Sci. 40, 18–32.CrossRefGoogle Scholar
  2. 2.
    Kritchevsky, D., Kolman, R. R., Guttmacher, R. M., and Forbes, M. (1959) Arch. Biochem. Biophys. 85, 444–451.PubMedCrossRefGoogle Scholar
  3. 3.
    Kritchevsky, D., Tepper, S. A., and Story, J. A. (1975) J. Food Sci. 40, 8–11.Google Scholar
  4. 4.
    Ebihara, K., Hirao, A., and Kiriyama, S. (1978) J. Agric. Chem. Soc. Jpn. 52(9)401–408.Google Scholar
  5. 5.
    Kritchevsky, D. (1978) Am. J. Clin. Nutr. 31, 565–574.Google Scholar
  6. 6.
    van Berge-Henegouwen, G. P., Huybregts, A. W., van de Werf, S., demacker, P., and Schade, R. W. (1979) Am. J. Clin. Nutr. 32, 794–798.PubMedGoogle Scholar
  7. 7.
    Jenkins, D. J. A., Reynolds, D., Slavin, B., Leeds, A. R., Jenkins, A. L. and Jepson, E. M. (1980) Am. J. Clin. Nutr. 33, 575–581.PubMedGoogle Scholar
  8. 8.
    Chen, W. L., Anderson, J. W., and Gould, M. R. (1981) Nutr. Rept. Int. 24, 1093–1098.Google Scholar
  9. 9.
    Story, J. A., Baldino, A., Czarnecki, S. K., and Kritchevsky, D. (1981) Nutr. Rept. Int. 24, 1213–1219.Google Scholar
  10. 10.
    Bordia, A. (1981) Am. J. Clin. Nutr. 34, 2100–2103.PubMedGoogle Scholar
  11. 11.
    Chi, M. S., Koh, E. T., and Stewart, T. J. (1982) J. Nutr. 112, 241–248.PubMedGoogle Scholar
  12. 12.
    Kamanna, V. S., and Chandrasekhara, N. (1982) Lipids 17, 483–486.PubMedGoogle Scholar
  13. 13.
    Kritchevsky, D., Tepper, S. A., Morrisey, R., and Klurfeld, D. (1980) Nutr. Rept. Int. 22, 641–645.Google Scholar
  14. 14.
    Chang, M. C., and Johnson, M. S. (1980) J. Nutr. 110, 931–936.PubMedGoogle Scholar
  15. 15.
    Stoll, A., and Seebeck, E. (1951) in Advances in Enzymology, (Nord, F. F., ed.) Vol. 11, pp. 377–400, Interscience, New York.Google Scholar
  16. 16.
    Qureshi, A. A., Din, Z. Z., Ahmad, Y., Elson, C. E., and Burger, W. C. (1983) J. Nutr. (in press).Google Scholar
  17. 17.
    Zahlten, R. N., and Stratman, F. W. (1974) Arch. Biochem. Biophys. 163, 600–608.PubMedCrossRefGoogle Scholar
  18. 18.
    Qureshi, A. A., Burger, W. C., Elson, C. E., and Benevenga, N. J. (1982) Lipids 17, 924–934.PubMedGoogle Scholar
  19. 19.
    Qureshi, A. A., Abuirmeileh, N., Burger, W. C., Din, Z. Z., and Elson, C. E. (1983) Atherosclerosis 46, 203–216.PubMedCrossRefGoogle Scholar
  20. 20.
    Gornall, A. G., Bardawill, C. J., and David, M. M. (1949) J. Biol. Chem. 177, 751–766.Google Scholar
  21. 21.
    Snedecor, G. W., and Cochran, W. G. (1971) in Statistical Method, 6th ed., pp. 258–298, The Iowa State University Press, Ames, IA.Google Scholar
  22. 22.
    Bordia, S. K., Verma, A. K., Vyas, B. L., Rathore, A. S., Bhu, N., and Bed, H. K. (1977) Atherosclerosis 26, 379–387.PubMedCrossRefGoogle Scholar
  23. 23.
    Miller, G. J., and Miller, N. E. (1975) Lancet 1, 16–19.PubMedCrossRefGoogle Scholar
  24. 24.
    Carew, T. E., Koschinsky, T., Hayes, S. B., and Steinberg, D. (1976) Lancet 1, 1315–1317.PubMedCrossRefGoogle Scholar
  25. 25.
    Subba Rao, D., Chandra Sekhara, N., Stayanarayana, M. N., and Srinvasan, M. (1970) J. Nutr. 100, 1307–1315.Google Scholar

Copyright information

© American Oil Chemists’ Society 1983

Authors and Affiliations

  • Asaf A. Qureshi
    • 1
    • 2
  • Naji Abuirmeileh
    • 4
  • Zafeer Z. Din
    • 1
    • 2
  • Charles E. Elson
    • 3
  • Warren C. Burger
    • 1
    • 2
  1. 1.USDA-ARS, Barley and Malt LaboratoryMadison
  2. 2.Department of AgronomyUniversity of WisconsinMadison
  3. 3.Department of Nutritional SciencesUniversity of WisconsinMadison
  4. 4.Department of Biological SciencesYarmuk UniversityIrbidJordan

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