, Volume 26, Issue 5, pp 405–406 | Cite as

Separation of cholesterol, and fatty acylglycerols, acids and amides by thin-layer chromatography

  • Alexander Bilyk
  • George J. Piazza
  • Raymond G. BistlineJr.
  • Michael J. Haas


A rapid unidimensional thin-layer chromatographic (TLC) method for the separation of neutral lipids is described, using two sequential solvent systems of different polarity. Excellent separations of mono-, di- and triglycerides, fatty acids, fatty amides, and cholesterol are thereby achieved. Separation is accomplished at room temperature and requires 25 min.


Oleic Acid Acylglycerols Triolein Standard Mixture Ricinoleic Acid 
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thin-layer chromatography


  1. 1.
    Morris, L.J. (1963)Lipid Res. 4, 357–359.Google Scholar
  2. 2.
    Mangold, H.K. (1969) inThin-Layer Chromatography (Stahl, E., ed.) pp. 363–421, Springer-Verlag, New York.Google Scholar
  3. 3.
    Wilgrube, H.J., Erb, W., and Boehler, E. (1973)Fette Seifen Anstrichm. 75, 168–169.CrossRefGoogle Scholar
  4. 4.
    Pernes, J.F., Nurit, Y., and de Heaulme, M. (1980)J. Chromatog. 181, 254–258.Google Scholar
  5. 5.
    Bilyk, A. (1981)J. Food. Sci. 46, 298–299.CrossRefGoogle Scholar
  6. 6.
    Duck-Chong, C.G., and Baker, G.J. (1983)Lipids 18, 387–389.PubMedGoogle Scholar
  7. 7.
    Kovács, L., Zalka, A., Dobó, R., and Pucsok, J. (1986)J. Chromatog. 382, 308–313.Google Scholar
  8. 8.
    Bitman, J., Wood, D.L., and Ruth, J.M. (1981)J. Liquid Chromatog. 4, 1007–1021.Google Scholar
  9. 9.
    Snyder, F. (1973)J. Chromatog. 82, 7–14.CrossRefGoogle Scholar
  10. 10.
    Krafft, F., and Stauffer, B., (1882)Ber. Dtsch. Chem. Ges. 15, 1728–1731.Google Scholar

Copyright information

© American Oil Chemists’ Society 1991

Authors and Affiliations

  • Alexander Bilyk
    • 1
  • George J. Piazza
    • 1
  • Raymond G. BistlineJr.
    • 1
  • Michael J. Haas
    • 1
  1. 1.ARS, Eastern Regional Research CenterU.S. Department of AgriculturePhiladelphia

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