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Lipids

, Volume 26, Issue 5, pp 405–406 | Cite as

Separation of cholesterol, and fatty acylglycerols, acids and amides by thin-layer chromatography

  • Alexander Bilyk
  • George J. Piazza
  • Raymond G. BistlineJr.
  • Michael J. Haas
Methods

Abstract

A rapid unidimensional thin-layer chromatographic (TLC) method for the separation of neutral lipids is described, using two sequential solvent systems of different polarity. Excellent separations of mono-, di- and triglycerides, fatty acids, fatty amides, and cholesterol are thereby achieved. Separation is accomplished at room temperature and requires 25 min.

Keywords

Oleic Acid Acylglycerols Triolein Standard Mixture Ricinoleic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

TLC

thin-layer chromatography

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Copyright information

© American Oil Chemists’ Society 1991

Authors and Affiliations

  • Alexander Bilyk
    • 1
  • George J. Piazza
    • 1
  • Raymond G. BistlineJr.
    • 1
  • Michael J. Haas
    • 1
  1. 1.ARS, Eastern Regional Research CenterU.S. Department of AgriculturePhiladelphia

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