Abstract
The incorporation of dietary cholestan-3β, 5α, 6β-triol (triol) into rat thoracic aortic tissue and changes in amino acid composition of the elastin were investigated to identify the cytotoxic properties of the triol. Weanling male Sprague-Dawley rats were fed the following diets for three months: (i) normal chow, (ii) normal chow with 1% (w/w) cholesterol added, or (iii) normal chow with 0.9% (w/w) cholesterol and 0.1% (w/w) triol added. Triol levels in the blood and in the thoracic aortic tissue were measured. Compositional changes of elastin were also determined. After three months on the triol-containing diet, triol was found in the thoracic aorta but was not detected in the blood. Amino acid analyses of the aortic tissue elastin revealed that the proline levels in the triol-fed animals were significantly greater than in the other two diet groups, while the elastin levels of leucine, aspartate, arginine, and phenylalanine decreased significantly. The mechanism for these observed changes induced by triol may reflect alternate splicing of elastin messenger ribonucleic acid (mRNA) resulting in structural changes in the elastin molecule. Dietary triol does contribute to tissue triol content and is associated with aortic elastin compositional changes. How these changes may contribute to the development of cardiovascular disease is not known.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ANOVA:
-
Analysis of variance
- α-epoxide:
-
cholesterol-5α, 6α-epoxide
- GLC:
-
gas-liquid chromatography
- HPLC:
-
high-performance liquid chromatography
- TMS:
-
trimethylsilyl ether
- triol:
-
cholestan-3β, 5α, 6β-triol
References
Baranowski, A., Adams, C.W.M., Bayliss High, O.B., and Bowyer, D.B. (1982)Atherosclerosis 41, 255–266.
Peng, S.K., Taylor, C.B., Hill, J.C., and Morin, R.J. (1985)Atherosclerosis 54, 121–133.
Cook, R.P., and MacDougall, J.D.B. (1968)Brit. J. Exptl. Pathol. 49, 265–271.
Matthias, D., Becker, C.H., Godicke, W., Schmidt, R., and Ponsold, K. (1987)Atherosclerosis 63, 115–124.
Jacobson, M.S. (1987)Lancet 2, part 1, 656–658.
van de Bovenkamp, P., Kosmeijer-Schuil, T.G., and Katan, M.B. (1988)Lipids 23, 1079–1085.
Maerker, G., Nungesser, E.H., and Bunick, F.J. (1988)Lipids 23, 761–765.
Bligh, E.G., and Dyer, W.J. (1959)Can. J. Biochem. Physiol. 37, 911–917.
Park, S.W., and Addis, P.B. (1986)J. Agric. Food Chem. 34, 653–659.
Chicoye, E., Powrie, W.D., and Fennema, O. (1968)J. Food Sci. 33, 581–587.
Jackson, D.S., and Cleary, E.G. (1967) inMethods if Biochemical Analysis (Glick, D., ed.) Vol. 15, pp. 25–76, Interscience Publishers, New York.
Sandberg, L.B., Wolt, T.B., and Leslie, J.G. (1986)Biochem. Biophys. Res. Commun. 136, 672–678.
Penke, B., Ferenczi, R., and Kovacs, K. (1974)Anal. Biochem. 60, 45–50.
Dixon, W.J. (chief editor), (1985)BMDP Statistical Software, University of California Press, Berkeley.
Peng, S.K., Tham, P., Taylor, C.B., and Mikkelson, B. (1979)Amer. J. Clin. Nutr. 32, 1033–1042.
Feldman, D.S., Hofmann, R., Gagnon, J., and Simpson, J. (1986) Statview 512+, pp. 127–136, Abacus Concepts, Inc., Calabasas, California.
Sandberg, L.B., Soskel, N.T., and Leslie, J.G. (1981)N. Engl. J. Med. 304, 566–579.
Pierce, R.A., Deak, S.B., Stolle, C.A., and Boyd, C.D. (1990)Biochemistry 29, 9677–9683.
Peng, S.K., Taylor, B., Tham, P., and Mikkelson, B. (1978)Arch. Pathol. Lab. Med. 102, 57–61.
Peng, S.K., Hill, J.C., Morin, R.J., and Taylor, C.B. (1985)Proc. Soc. Exptl. Biol. Med. 180, 126–132.
Author information
Authors and Affiliations
About this article
Cite this article
Blankenship, J.W., Van Gent, C.M., Sandberg, L.B. et al. Oxysterol incorporation into rat aorta resulting in elastin compositional changes. Lipids 26, 381–384 (1991). https://doi.org/10.1007/BF02537203
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02537203