Skip to main content
SpringerLink
Log in

Effects of dietary oils and methyl ethyl ketone peroxide onin vivo lipid peroxidation and antioxidants in rat heart and liver

  • Article
  • Published:
Lipids

Abstract

Weanling male Sprague-Dawley rats were fed diets for four weeks which differed in their content of n−6 (corn oil; CO) and n−3 fatty acids (fish oil; FO), but were similar in their content of saturated and monounsaturated fatty acids and vitamin E. At the end of the four-week feeding period, each dietary group was subdivided into two groups. One group received a single placebo injection of α-tocopherol-stripped corn oil (TSCO); the other group received a single injection of the free radical generator, methyl ethyl ketone peroxide (MEKP), in TSCO. Twenty-four hours after injection, the effect of dietary oil and MEKP treatment on endogenous lipid peroxide (LPO) production (measured as methylene blue formed by the “Determiner LPO” assay), glutathione (GSH) and vitamin E content, and fatty acid composition of phosphatidylcholine and phosphatidylethanolamine in heart and liver from unfasted animals were measured. FO-fed rats had significantly heavier hearts and livers, increased levels of n−3 fatty acids in membrane phospholipids, and higher liver LPO levels than CO-fed rats. MEKP treatment resulted in significantly lower body weights and liver GSH levels. The data indicate that dietary n−3 fatty acids increase lipid peroxidation in liver somewhat more than in heart. The study also demonstrates that the effect of induced oxidative stress due to a single dose of MEKP on lipid peroxide formation and antioxidant status in tissues from unfasted animals was independent of the dietary oils.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CO:

corn oil

FO:

fish oil

GSH:

glutathione

GSSH:

oxidized GSH

HPLC:

high-performance liquid chromatography

i.p.:

intraperitoneal

LPO:

lipid peroxide

LSD:

least significant difference

MEKP:

methyl ethyl ketone peroxide

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PI:

peroxidizability index

PUFA:

polyunsaturated fatty acids

TBARS:

thiobarbituric acid reactive substances

TBHQ:

tertiary butylhydroquinone

TSCO:

α-tocopherol stripped corn oil

References

  1. Herbert, K.E., and Wills, E.D. (1987)Biochem. Soc. Trans. 15, 410–411.

    CAS  Google Scholar 

  2. Hu, M.-L., Frankel, E.N., Leibovitz, B.E., and Tappel, A.L., (1989)J. Nutr. 119, 1574–1582.

    PubMed  CAS  Google Scholar 

  3. Mouri, K., Ikesu, H., Esaka, T., and Igarashi, O. (1984)J. Nutr. Sci. Vitaminol. 30, 307–318.

    PubMed  CAS  Google Scholar 

  4. Steinberg, D., Parthasarathy, S., Carew, T.E., Khoo, J.C., and Witztum, J.L. (1989)N. Engl. J. Med. 320, 915–924.

    Article  PubMed  CAS  Google Scholar 

  5. Benediktsdottir, V.E., and Gudbjarnason, S. (1988)J. Lipid Res. 29, 765–772.

    PubMed  CAS  Google Scholar 

  6. Nalbone, G., Leonardi, J., Termine, E., Portugal, H., Lechene, P., Pauli, A.-M., and Lafont, H. (1989)Lipids 24, 179–186.

    Article  PubMed  CAS  Google Scholar 

  7. Burton, K.P., Morris, A.C., Massey, K.D., Buja, L.M., and Hagler, H.K. (1990)J. Mol. Cell. Cardiol. 22, 1035–1047.

    Article  PubMed  CAS  Google Scholar 

  8. L'Abbé, M.R., Trick, K.D., and Beare-Rogers, J.L. (1991)J. Nutr. 121, 1331–1340.

    PubMed  Google Scholar 

  9. Pascoe, G.A., Fariss, R.W., Olafsdottir, K., and Reed, D.J. (1987)Eur. J. Biochem. 166, 241–247.

    Article  PubMed  CAS  Google Scholar 

  10. Warren, D.L., and Reed, D.J. (1991)Arch. Biochem. Biophys. 285, 45–52.

    Article  PubMed  CAS  Google Scholar 

  11. Siegers, C.-P., Hubscher, W., and Younes, M. (1982)Res. Commun. Chem. Path. Pharmacol. 37, 163–169.

    CAS  Google Scholar 

  12. Farooqui, M.Y.H., and Ahmed, A.E. (1984)Life Sciences 34, 2413–2418.

    Article  PubMed  CAS  Google Scholar 

  13. Yalçin, A.S., Koçak-Tocer, N., Uysal, M., Aykaç, G., Sivas, A., and Öz, H. (1986)J. Appl. Toxicol. 6, 303–306.

    Article  Google Scholar 

  14. Godin, D.V., and Wohaieb, S.A. (1988)Free Radical Biol. Med. 5, 165–176.

    Article  CAS  Google Scholar 

  15. Lauterburg, B.H., Adams, J.D., and Mitchell, J.R. (1984)Hepatology 4, 586–590.

    PubMed  CAS  Google Scholar 

  16. Xia, Y., Hill, K.E., and Burk, R.F. (1985)J. Nutr. 115, 733–742.

    PubMed  CAS  Google Scholar 

  17. Wohaieb, S.A., and Godin, D.V. (1987)Diabetes 36, 169–173.

    PubMed  CAS  Google Scholar 

  18. Ceconi, C., Cargnoni, A., Pasini, E., Condorelli, E., Curello, S., and Ferrari, R. (1991)Am. J. Physiol. 260, H1057-H1061.

    PubMed  CAS  Google Scholar 

  19. Ceconi, C. (1992)Am. Physiol. Soc. 32, H982-H983.

    Google Scholar 

  20. Turini, M.E., Thomson, A.B.R., and Clandinin, M.T. (1991)Lipids 26, 431–441.

    PubMed  CAS  Google Scholar 

  21. Ohishi, N., Ohkawa, H., Miike, A., Tatano, T., and Yagi, K. (1985)Biochem. International 10, 205–211.

    CAS  Google Scholar 

  22. Litov, R.E., Matthews, L.C., and Tappel, A.L. (1981)Toxicol. Appl. Pharmacol. 59, 96–106.

    Article  PubMed  CAS  Google Scholar 

  23. Ando, M., and Tappel, A.L. (1985)Chem.-Biol. Interactions 55, 317–326.

    Article  CAS  Google Scholar 

  24. American Institute of Nutrition (1977)J. Nutr. 107, 1340–1348.

    Google Scholar 

  25. Bligh, E.G., and Dyer, W.J. (1959)Can. J. Biochem. Physiol. 37, 911–917.

    PubMed  CAS  Google Scholar 

  26. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  27. Fariss, M.W., and Reed, D.J. (1987)Methods Enzymol. 143, 101–109.

    Article  PubMed  CAS  Google Scholar 

  28. Ingold, K.U., Burton, G.W., Foster, D.O., Hughes, L., Lindsay, D.A., and Webb, A. (1987)Lipids 22, 163–172.

    Article  PubMed  CAS  Google Scholar 

  29. SAS (1985) SAS/STATTM Guide for Personal Computers (Version 6) SAS Inst. Inc., Cary.

    Google Scholar 

  30. Snedecor, G.W., and Cochran, W.G. (1989)Statistical Methods, 8th edn., Iowa State Press, Ames.

    Google Scholar 

  31. Singh, Y., Hall, G.L., and Miller, M.G. (1992)J. Biochem. Toxicol. 7, 97–105.

    Article  PubMed  CAS  Google Scholar 

  32. Post, J.A., Langer, G.A., Op den Kamp, J.A.F., and Verkleij, A.J. (1988)Biochim. Biophys. Acta 943, 256–266.

    Article  PubMed  CAS  Google Scholar 

  33. Chautan, M., Calaf, R., Léonardi J., Charbonnier, M., Andre, M., Portugal, H., Pauli, A.-M., Lafont, H., and Nalbone, G. (1990)J. Lipid Res. 31, 2201–2208.

    PubMed  CAS  Google Scholar 

  34. Scholz, R.W., Graham, K.S., Wynn, M.K., and Reddy, C.C. (1990) inBiological Oxidation Systems (Reddy, C.C., Hamilton, G.A., and Madyastha, K.M., eds.) Vol. 2, pp. 841–867, Academic Press, San Diego.

    Google Scholar 

  35. Lucy, J.A. (1972)Ann. N.Y. Acad. Sci. 203, 4–11.

    PubMed  CAS  Google Scholar 

  36. Sevanian, A. (1988) inCellular Antioxidant Defense Mechanisms (Chow, C.K., ed.) Vol. II, pp. 77–95, CRC Press Inc., Boca Raton.

    Google Scholar 

  37. Higashi, T., Tateishi, N., Naruse, A., and Sakamoto, Y. (1977)J. Biochem. 82, 117–124.

    PubMed  CAS  Google Scholar 

  38. Deleve, L.D., and Kaplowitz, N. (1990)Semin. Liver Dis. 10, 251–266.

    Article  PubMed  CAS  Google Scholar 

  39. Gibson, D.D., Hawrylko, J., and McCay, P.B. (1985)Lipids 20 704–711.

    Article  PubMed  CAS  Google Scholar 

  40. Brigelius, R. (1983)Hoppe-Seyler's Z. Physiol. Chem. 364, 989–996.

    PubMed  CAS  Google Scholar 

  41. Strubelt, O., Dost-Kempf, E., Siegers, C.-P., Younes, M., Völpel, M., Preuss, U., and Dreckmann, J.G. (1981)Toxicol. Appl. Pharmacol. 60, 66–77.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Science Institute, University of Iceland, Dunhaga 3, IS-107, Reykjavík, Iceland

    Gudrún V. Skúladóttir

  2. Department of Nutrition and Food Management, Oregon State University, 97331, Corvallis, Oregon

    Du Shi-Hua & Rosemary C. Wander

  3. Department of Biochemistry and Biophysics, Oregon State University, 97331, Corvallis, Oregon

    Ann E. Brodie & Donald J. Reed

Authors
  1. Gudrún V. Skúladóttir
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Du Shi-Hua
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ann E. Brodie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Donald J. Reed
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rosemary C. Wander
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Skúladóttir, G.V., Shi-Hua, D., Brodie, A.E. et al. Effects of dietary oils and methyl ethyl ketone peroxide onin vivo lipid peroxidation and antioxidants in rat heart and liver. Lipids 29, 351–357 (1994). https://doi.org/10.1007/BF02537189

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02537189

Keywords

Search

Navigation