Abstract
The distribution of dolichol in rat liver was studied. Upon high-speed centrifugation, 9% of the total tissue dolichol was recovered in the supernatant. Dolichol was enclosed in vesicles and in lipidic particles which were isolated by gel filtration and density gradient centrifugation. The particles had a diameter of 20 nm and contained dolichol, ubiquinone, cholesterol, phospholipid and some protein. Similar particles were recovered upon incubation of isolated hepatocytes with liposomes containing dolichol. From the lysosomal lumen, lipid particles containing dolichol, ubiquinone, cholesterol and phospholipid, but no protein, were isolated. The diameter of the particles was 20–40 nm with a molecular weight of 130 kDa. Puromycin treatment inhibited protein synthesis, but did not affect dolichol transfer from the endoplasmic reticulum to lysosomes, suggesting that the transfer is not mediated by newly synthesized apoprotein. The results indicate that a sizeable portion of the total cellular dolichol is present in cytoplasm and in lysosomal lumen. Furthermore, dolichol probably participates in the translocation process.
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Abbreviations
- EGTA:
-
ethylene-bis(oxyethylenenitrilo)tetraacetic acid
- HPLC:
-
high-performance liquid chromatography
- SDS:
-
sodium dodecyl sulfate
References
Valtersson, C., van Duijn, G., Verklej, A.J., Chojnacki, T., de Kruijff, B., and Dallner, G. (1985)J. Biol. Chem. 260, 2742–2751.
Quinn, P.J., and Katsikas, H. (1985) inCoenzyme Q: Biochemistry, Bioenergetics and Clinical Applications of Ubiquinone (Lenaz, G., ed.) pp. 107–130, John Wiley, Salisbury.
van Dijck, P.W.M., de Kruijff, B., van Deenen, L.L.M., De Gier, J., and Demel, R.M. (1976)Biochim. Biophys. Acta 455, 576–587.
Dallner, G., and de Pierre, J.W. (1983)Methods Enzymol. 96, 542–557.
Edlund, C., Ericsson, J., and Dallner, G. (1987)Chem.-Biol. Interactions 62, 191–208.
Keller, R.K., and Adair, W.L. (1980)J. Biol. Chem. 255, 8618–8622.
Zilversmit, D.B. (1984)J. Lipid Res. 25, 1563–1569.
Struck, D.K., and Lennarz, W.J. (1980) inThe Biochemistry of Glycoproteins and Proteoglycans (Lennarz, W.J., ed.) pp, 35–83. Plenum Press, New York.
Ekström, T.J., Chojnacki, T., and Dallner, G. (1984)J. Biol. Chem. 259, 10460–10468.
Ekström, T.J., Chojnacki, T., and Dallner, G. (1987)J. Biol. Chem. 262, 4090–4097.
Wattiaux, R., Wattiaux-de Coninck, S., Ronveaux-Dupaland, M.F., and Dubois, F. (1978)J. Cell Biol. 78, 349–368.
Kalén, A., Appelkvist, E.L., Chojnacki, T., and Dallner, G. (1990)J. Biol. Chem. 265, 1158–1164.
Keenan, R., and Kruczek, H. (1975)Anal. Biochem. 69, 504–509.
Autuori, F., Brunk, U., Peterson, E., and Dallner, G. (1982)J. Cell Sci. 57, 1–13.
Elmberger, P.G., Eggens, I., and Dallner, G. (1989)Biomed. Chromatography 3, 20–28.
Elmberger, P.G., Kalén, A., Appelkvist, E.L., and Dallner, G. (1987)Eur. J. Biochem. 168, 1–11.
Valtersson, C., Filipsson, L., and Dallner, G. (1986)J. Lipid Res. 27, 731–741.
Laemmli, U.K. (1970)Nature 227, 680–685.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.
Ekström, T., Chojnacki, T., and Dallner, G. (1982)J. Lipid Res. 23, 972–982.
Omura, T. (1980)Pharmacol. Ther. 8, 489–499.
Eggens, I., Chojnacki, T., Kenne, L., and Dallner, G. (1983)Biochim. Biophys. Acta 751, 355–368.
de Ropp, J.S., and Troy, F.A. (1985)J. Biol. Chem. 260, 15669–15674.
Tollbom, Ö., and Dallner, G. (1986)Br. J. Exp. Path. 67, 757–764.
Palade, G. (1975)Science 189, 347–358.
Bishop, W.R., and Bell, R.M. (1988)Ann. Rev. Cell Biol. 4, 579–610.
Goldstein, J.L., and Brown, M.S. (1977)Ann. Rev. Biochem. 46, 897–930.
Van Dessel, G., De Wolf, M., Lagrou, A., Hilderson, H.J., and Dierick, W. (1986)Biochim. Biophys. Acta 878, 338–345.
Tollbom, Ö., Valtersson, C., Chojnacki, T., and Dallner, G. (1988)J. Biol. Chem. 263, 1347–1352.
Tabas, I., and Kornfeld, S. (1980)J. Biol. Chem. 255, 6633–6639.
Banerjee, D., and Redman, C.M. (1984)J. Cell Biol. 99, 1917–1926.
Coleman, R. (1987)Biochem. J. 244, 249–261.
Elmberger, P.G., Engfeldt, P., and Dallner, G. (1988)J. Lipid Res. 29, 1651–1662.
Elmberger, P.G., Kalén, A., Brunk, U., and Dallner, G. (1989)Lipids 24, 919–930.
Appelkvist, E.L., and Kalén, A. (1989)Eur. J. Biochem. 185, 503–509.
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Löw, P., Peterson, E., Edlund, C. et al. Nonmembrane associated dolichol in rat liver. Lipids 27, 1–9 (1992). https://doi.org/10.1007/BF02537050
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DOI: https://doi.org/10.1007/BF02537050