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Inhibition of soybean lipoxygenase-1 by chain-breaking antioxidants

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Lipids

Abstract

The aim of this investigation was to determine whether chain-breaking antioxidants able to prevent lipid peroxidation can inhibit lipoxygenase-1 (EC 1.13.11.12). Therefore, the effects of ascorbic acid, 6-palmitoylascorbic acid and trolox on the enzyme activity were analyzed by means of Lineweaver-Burk double reciprocal plots and Yoshino's graphical method. The effect of these compounds on the formation of free radicals during lipoxygenase-1 reaction was investigated as well, by monitoring the enzymic formation of oxodienes. We present evidence that the chain-breaking antioxidants ascorbic acid, 6-palmitoylascorbic acid and trolox inhibit soybean lipoxygenase-1 in the micromolar concentration range (Ki 27, 3 and 18 μM, respectively). The inhibition is competitive, complete and reversible. All three compounds trap the free radicals formed during the lipoxygenase-catalyzed reaction, which might substantially contribute to their inhibitory ability. These findings can have physiological significance in the light of the lipoxygenase involvement in biomembrane remodelling.

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Abbreviations

LOX:

lipoxygenase-1

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Authors and Affiliations

  1. IDI-IRCCS Unit, University of Rome “Tor Vergata”, Via di Tor Vergata n. 135, I-00133, Rome, Italy

    Mauro Maccarrone

  2. Department of Experimental Medicine and Biochemical Science, University of Rome “Tor Vergata”, Via di Tor Vergata n. 135, I-00133, Rome, Italy

    Alessandro Finazzi Agrò

  3. Bijvoet Center for Biomolecular Research, Department of Bio-Organic Chemistry, Utrecht University, NL-3584 CH, Utrecht, The Netherlands

    Mauro Maccarrone, Gerrit A. Veldink & Johannes F. G. Vliegenthart

Authors
  1. Mauro Maccarrone
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  2. Gerrit A. Veldink
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  3. Johannes F. G. Vliegenthart
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  4. Alessandro Finazzi Agrò
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Maccarrone, M., Veldink, G.A., Vliegenthart, J.F.G. et al. Inhibition of soybean lipoxygenase-1 by chain-breaking antioxidants. Lipids 30, 51–54 (1995). https://doi.org/10.1007/BF02537041

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  • DOI: https://doi.org/10.1007/BF02537041

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