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Occurrence of 22∶3n−9 and 22∶4n−9 in the lipids of the topminnow (Poeciliopsis lucida) hepatic tumor cell line, PLHC-1

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Lipids

Abstract

The lipids of the hepatic tumor cell line, PLHC-1, from the topminnow (Poeciliopsis lucida), were found to contain considerable amounts of a range of n−9 polyunsaturated fatty acids despite culture in serum containing significant amounts of essential fatty acids. The structural identity of all the n−9 polyunsaturated fatty acids was confirmed by gas chromatography/mass spectrometry. Of particular interest, PLHC-1 cell total lipid contained 1.9% of 22∶3n−9 and 3.3% of 22∶4n−9. As the culture medium contained virtually no n−9 polyunsaturated fatty acids, these fatty acids are all formed by the PLHC-1 cells, presumably form 18∶1n−9. The 22∶3n−9 and 22∶4n−9 are presumably formed by processes of elongation and “Δ4” desaturation of Mead acid, 20∶3n−9, present at over 11% in fatty acids of total lipid. Both 22∶3n−9 and 22∶4n−9 were primarily located in phosphatidylserine (4.1 and 8.5% respectively) and, to a lesser extent, in phosphatidylethanolamine (2.2 and 6.5%, respectively), in common with the C22 derivatives of the n−3 and n−6 series, whereas 20∶3n−9 was preferentially located in phosphatidylinositol (31.2%). The results establish that long-chain polyunsaturated fatty acids of the n−9 series can be formed in vertebrate tissue other than in conditions of classical essential fatty acid deficiency.

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Abbreviations

BHT:

butylated hydroxytoluene

BSA:

bovine serum albumin

CI:

chemical ionization

EFA:

essential fatty acids

EI:

electron ionization

FAME:

fatty acid methyl esters

FCS:

fetal calf serum

GC:

gas chromatography

HBSS:

Hank's balanced salt solution (without Ca2+ and Mg2+)

MS:

mass spectrometry

PC:

phosphatidylcholine

PE:

phosphatidyl-ethanolamine

PI:

phosphatidylinositol

PS:

phosphatidylserine

PUFA:

polyunsaturated fatty acid

TF:

turbot fin cell line

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Authors and Affiliations

  1. NERC Unit of Aquatic Biochemistry, Dept. of Biological and Molecular Sciences, University of Stirling, FK9 4LA, Stirling, Scotland, UK

    Douglas R. Tocher, James R. Dick & John R. Sargent

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  1. Douglas R. Tocher
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  2. James R. Dick
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Tocher, D.R., Dick, J.R. & Sargent, J.R. Occurrence of 22∶3n−9 and 22∶4n−9 in the lipids of the topminnow (Poeciliopsis lucida) hepatic tumor cell line, PLHC-1. Lipids 30, 555–565 (1995). https://doi.org/10.1007/BF02537031

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  • DOI: https://doi.org/10.1007/BF02537031

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