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The effect of culture medium composition on ether lipid cytotoxic activity

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Lipids

Abstract

The effect of a serum-free medium (TNB-100), compared to RPMI 1640 containing 10% fetal bovine serum (FBS), on the lipid composition of HL60 and K562 leukemic cells was investigated. The 10% FBS RPMI medium contained approximately three times more phospholipids (PL), about three times more protein and eight times more cholesterol (CHOL) than did the TNB-100 medium. Cells cultured in TNB-100 medium, referred to as HL60-TNB and K562-TNB cells, were significantly lower in PL and CHOL than 10% FBS RPMI cells, with about a threefold higher PL-to-CHOL ratio; however, these cells were significantly higher in protein content. Cells grown in TNB-100 were also significantly more fluid than 10% FBS RPMI cells and were more sensitive to the fluidizing action of the ether lipid 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine. The 50% inhibitory dose of the drug was about 50% lower in TNB-grown cells than in 10% FBS RPMI cells.

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Abbreviations

CHOL:

cholesterol

DPH:

1,6-diphenyl-1,3,5-hexatriene

EL:

ether lipids

FBS:

fetal bovine serum

ET-18-OMe:

1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine

ID50 :

50% inhibitory dose

PBS:

phosphate buffered saline

PL:

phospholipid(s)

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Authors and Affiliations

  1. Department of Biochemistry, Boston University School of Medicine, 02118, Boston, Massachusetts

    Edward J. Modest

  2. Istituto di Richerche Farmacologiche “Mario Negri”, Via Eritrea 62, 20157, Milan, Italy

    Luisa Diomede, Bianca Piovani & Mario Salmona

Authors
  1. Luisa Diomede
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  2. Bianca Piovani
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  3. Edward J. Modest
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  4. Mario Salmona
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Diomede, L., Piovani, B., Modest, E.J. et al. The effect of culture medium composition on ether lipid cytotoxic activity. Lipids 28, 189–192 (1993). https://doi.org/10.1007/BF02536638

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  • DOI: https://doi.org/10.1007/BF02536638

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