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Steryl esters in a cell suspension culture of celery (Apium graveolens)

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Lipids

Abstract

Arange of analytical techniques was used to investigate the composition of the steryl fatty acyl esters in a cell suspension culture of celery (Apium graveolens). Gas chromatography (GC) and GC-mass spectrometry (GC-MS), using electron ionization (EI) and negative ion chemical ionization (NICI), were employed to characterize the intact steryl esters. Assignments were supported by analysis of the sterol and fatty acid moieties released from the intact molecular species by alkaline hydrolysis. A selectivity for sterol esterification was noted, with the major free sterol, stigmasterol, occurring only in a very small amount in the esterified form. Instead, the precursors to Δ5-phytosterols, particularly cycloartenol, predominated in the ester fraction. The pentacyclic triterpene, β-amyrin, was also found as the palmitate and linoleate esters. Changes in composition and abundance of the steryl esters during the different growth phases of a celery cell suspension culture were investigated. The total amount of esterified sterols exceeded that of free sterols throughout the growth cycle. The changes observed during growth highlighted differences between the esters of precursor sterols and those of the 4-desmethyl-sterols, and it is postulated that the various steryl esters perform different functions in cell metabolism.

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Abbreviations

EI:

electron impact ionization

FS:

free sterols

GC:

gas chromatography

M+ :

molecular ion

MS:

mass spectrometry

NICI:

negative ion chemical ionization

SAM:

S-adenosyl-L-methionine

SE:

steryl esters

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Authors and Affiliations

  1. Department of Biochemistry, University of Liverpool, L69 3BX, Liverpool, UK

    Lynne Dyas, Mark C. Prescott, Richard P. Evershed & L. John Goad

Authors
  1. Lynne Dyas
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  2. Mark C. Prescott
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  3. Richard P. Evershed
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  4. L. John Goad
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Dyas, L., Prescott, M.C., Evershed, R.P. et al. Steryl esters in a cell suspension culture of celery (Apium graveolens). Lipids 26, 536–541 (1991). https://doi.org/10.1007/BF02536600

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  • DOI: https://doi.org/10.1007/BF02536600

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