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Acylation of lyso platelet-activating factor by splenocytes of the rainbow trout,Oncorhyncus mykiss

  • Published:
Lipids

Abstract

In mammalian systems, platelet-activating factor, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, (PAF) is rapidly inactivated by a deacetylation/reacylation system that produces 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine which is highly enriched in arachidonic acid. There is some evidence that n−3 fatty acids may have an impact on this system in humans but the nature of this impact is unclear. In rainbow trout, n−3 fatty acids are known to be essential dietary components which are derived through the food chain. Substantial quantities of n−3 fatty acids are found in trout membrane phospholipids. We show here that in sharp contrast to mammalian cells, trout cells acylate lyso platelet-activating factor, alkyl-GPC, 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine, (lyso-PAF) with a high degree of specificity for n−3 fatty acids. When [3H]lysoPAF was incubated with these cells, only three molecular species of alkylacylglycerophosphocholine were produced, and 92% contained n−3 fatty acids. Since isolated membranes yielded similar results, it appears that the acylation proceedsvia a coenzyme A-independent transacylase as found in mammalian systems.

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Abbreviations

alkylacyl-GPC:

1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine

DHA:

docosahexaenoate

HPLC:

high-performance liquid chromatography

lysoPAF:

lyso platelet-activating factor, alkyl-GPC, 1-O-alkyl-sn-glycero-3-phosphocholine, 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine

PAF:

platelet-activating factor, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine

PC:

phosphatidylcholine

PUFA:

polyunsaturated fatty acids

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Mountain Aquaculture Research Center, Western Carolina University, 28723, Cullowhee, North Carolina

    G. L. Pool, B. Samples, M. R. Turner & R. H. Lumb

Authors
  1. G. L. Pool
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  2. B. Samples
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  3. M. R. Turner
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  4. R. H. Lumb
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Pool, G.L., Samples, B., Turner, M.R. et al. Acylation of lyso platelet-activating factor by splenocytes of the rainbow trout,Oncorhyncus mykiss . Lipids 26, 517–520 (1991). https://doi.org/10.1007/BF02536596

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  • DOI: https://doi.org/10.1007/BF02536596

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