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Effect of acylation stimulating protein on the triacylglycerol synthetic pathway of human adipose tissue

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Lipids

Abstract

Acylation stimulating protein (ASP) is a 14 kDa plasma protein which causesin vitro triacylglycerol synthesis in human adipocytes and fibroblasts to increase substantially. ASP was found to stimulate human adipose tissue microsomal glycerophosphate acyltransferase and diacylglycerol acyltransferase activities by 23% and 90%, respectively. However, phosphatidate phosphohydrolase activity showed no increase in activity, nor did microsomal acyl-CoA synthetase activity. Moreover, ASP did not decrease the apparent Km of diacylglycerol acyltransferase (DGAT), but rather increased its apparent Vmax suggesting direct interaction of ASP with DGAT.

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Abbreviations

ASP:

acylation stimuating protein

BSA:

bovine serum albumin

DGAT:

diacylglycerol acyltransferase

DTT:

dithiothreitol

FABP:

fatty acid binding protein

GPAT:

glycerophosphate acyltransferase

PAPH:

phosphatidate phosphohydrolase

TLC:

thin-layer chromatography

References

  1. Cianflone, K.M., Sniderman, A.D., Walsh, M.J., Vu, H.T., Gagnon, J., and Rodriguez, M.A. (1989)J. Biol. Chem. 264, 426–430.

    PubMed  CAS  Google Scholar 

  2. Walsh, M.J., Sniderman, A.D., Cianflone, K.M., Vu, H.T., Rodriguez, M.A., and Forse, R.A. (1989)J. Surg. Res. 46, 470–473.

    Article  PubMed  CAS  Google Scholar 

  3. Cianflone, K.M., Vu, H.T., Walsh, M., Baldo, A., and Sniderman, A.D. (1989)J. Lipid Res. 30, 1727–1733.

    PubMed  CAS  Google Scholar 

  4. Cianflone, K.M., Rodriguez, M.A., Walsh, M., Vu, H.T., and Sniderman, A.D. (1988)Clin. Invest. Med. 11, 99–107.

    PubMed  CAS  Google Scholar 

  5. Cianflone, K.M., Maslowska, M.H., and Sniderman, A.D. (1990)J. Clin. Invest. 85, 722–730.

    Article  PubMed  CAS  Google Scholar 

  6. Jarett, L. (1979)Meth. Enzymol. 31A, 60–71.

    Google Scholar 

  7. Walsh, M., Durocher, V., and Rodriguez, M.A. (1989)Biochem. Cell Biol. 67, 48–52.

    Article  PubMed  CAS  Google Scholar 

  8. Bar-Tana, J., Rose, G., and Shapiro, B. (1971)Biochem. 122, 353–362.

    CAS  Google Scholar 

  9. Jamdar, S.C., and Fallon, H.J. (1973)J. Lipid Res. 14, 509–516.

    PubMed  CAS  Google Scholar 

  10. Ames, B.N., and D.T. Dubin (1960)J. Biol. Chem. 235, 769–775.

    PubMed  CAS  Google Scholar 

  11. Bradford, M. (1976)Anal. Biochem. 72, 248–254.

    Article  PubMed  CAS  Google Scholar 

  12. Mishkin, S., and Turcotte, R. (1974)Biochem. Biophys. Res. Commun. 60, 376–381.

    Article  PubMed  CAS  Google Scholar 

  13. O'Doherty, P.J.A., and Kuksis, A. (1975)FEBS Lett. 60, 256–258.

    Article  PubMed  Google Scholar 

  14. Burnett, D.A., Lysenko, N., Manning, J.A., and Ockner, R.K. (1979)Gastroenterol. 77, 241–249.

    CAS  Google Scholar 

  15. Manley, E.R., Skrdlant, H.B., Hansbury, E., and Scallen, T.J. (1974)Biochem. Biophys. Res. Commun. 58, 229–235.

    Article  PubMed  CAS  Google Scholar 

  16. Roncari, D.A.K., and Mack, E.Y.W. (1981)Can. J. Biochem. 59, 944–950.

    Article  PubMed  CAS  Google Scholar 

  17. Glatz, J.F.C., and Veerkamp, J.H. (1985)Int. J. Biochem. 17, 13–22.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. The McGill Unit for the Prevention of Cardiovascular Disease, McGill University, Royal Victoria Hospital, H3A 1A1, Montreal, Quebec, Canada

    Zivart Yasruel, Katherine Cianflone, Allan D. Sniderman, Mark Rosenbloom, Mark Walsh & Miguel A. Rodriguez

Authors
  1. Zivart Yasruel
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  2. Katherine Cianflone
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  3. Allan D. Sniderman
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  4. Mark Rosenbloom
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  5. Mark Walsh
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  6. Miguel A. Rodriguez
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Yasruel, Z., Cianflone, K., Sniderman, A.D. et al. Effect of acylation stimulating protein on the triacylglycerol synthetic pathway of human adipose tissue. Lipids 26, 495–499 (1991). https://doi.org/10.1007/BF02536592

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  • DOI: https://doi.org/10.1007/BF02536592

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