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Inhibitor(s) of platelet-activating factor (PAF) in human saliva

  • PAF Analysis by Spectrometric and Immunological Assay
  • Published:
Lipids

Abstract

Quantitation of platelet-activating factor (PAF) in human saliva samples by radioimmunoassay indicated there was, at times, sufficient PAF present to aggregate platelets. However, in certain samples, we observed little or no aggregation, and furthermore, these samples were found to inhibit aggregation induced by PAF (200 pg). Chromatographic fractionation of pooled saliva increased the PAF activity 4-fold, and the observed inhibitory activity was found to co-migrate with the fatty acids. The inhibitory fraction was found to be active against platelet aggregation induced by arachidonic acid (3.4 nmole) as well as PAF (25 pg), but not thrombin (20 mU). These results indicate the existence of a PAF inhibitor in saliva, which may explain why potentially toxic levels of PAF can occur in the saliva of normal, healthy individuals. These findings also highlight an important advantage of the radioimmunoassay over platelet aggregation for the quantitation of PAF in, at least, some biological fluids.

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Abbreviations

AA:

arachidonic acid

BSA:

bovine serum albumin

C:

cholesterol

CE:

cholesteryl esters

FA:

fatty acids

MG:

monoglycerides

PAF:

platelet-activating factor

PBS:

phosphate (50mM) buffered saline pH 7.2

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PL:

phospholipids

RIA:

radioimmunoassay

S:

sphingomyelin

TG:

triglycerides

TLC:

thin-layer chromatography

U:

unknown

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Authors and Affiliations

  1. Kolling Institute of Medical Research, Royal North Shore Hospital, 2065, St. Leonards, N.S.W.

    Mary A. Smal & Brian A. Baldo

  2. Department of Medicine, University of Sydney, 2006, N.S.W., Australia

    Brian A. Baldo

Authors
  1. Mary A. Smal
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  2. Brian A. Baldo
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Smal, M.A., Baldo, B.A. Inhibitor(s) of platelet-activating factor (PAF) in human saliva. Lipids 26, 1144–1147 (1991). https://doi.org/10.1007/BF02536519

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  • DOI: https://doi.org/10.1007/BF02536519

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