Skip to main content
SpringerLink
Log in

Platelet-activating factor acetylhydrolase activity in human tissues and blood cells

  • PAF Metabolism and Its Regulation
  • Published:
Lipids

Abstract

Human tissues, blood cells, and plasma have enzymes that catalyze the hydrolysis of PAF (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine). The activities are not due to phospholipases A2 that hydrolyze long chain acyl groups at thesn-2 position of glycerophospholipids, since they are calcium-independent and are specific for hydrolysis of short chain acyl groups. We examined the biochemical properties of these PAF acetylhydrolase activities (EC 3.1.1.47) in homogenates of human liver and spleen, in white blood cells (neutrophils and monocytes), and in erythrocytes. The data suggest that the plasma and intracellular PAF acetylhydrolase activities are likely due to different proteins. Second, the intracellular PAF acetylhydrolase activities in liver and spleen share several biochemical features that differentiate them from the activities in blood cells. Third, the activities in monocytes and neutrophils have properties that differentiate them from the activity present in human erythrocytes. Finally, the erythrocyte activity has unique properties that place it in a separate category of short chain acylhydrolases. In conclusion, there is a family of distinct enzymes that can be identified as PAF acetylhydrolases based on their calcium-independence and specificity for a short residue at thesn02 position of phospholipids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

p-BPB:

p-bromophenacylbromide

DEPC:

diethylpyrocarbonate

DTNB:

5,5′-dithio-bis(2-nitrobenzoic acid)

EDTA:

ethylenediaminetetraacetic acid

EGTA:

ethylene glycol-bis(β-aminoethyl ether)N,N,N′,N′-tetraacetic

LDL:

low density lipoprotein

lysoPAF:

1-O-alkyl-sn-glycero-3-phosphocholine

PAF:

1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine

References

  1. Mueller, H.W., O'Flaherty, J.T., and Wykle, R.L. (1983)J. Biol. Chem. 258, 6213–6218.

    PubMed  CAS  Google Scholar 

  2. Malavasi, F., Tetta, C., Funaro, A., Bellone, G., Ferrero, E., Colli Franzone, A., Dellabona, P., Rusci, R., Matera, L., Camussi, G., and Caligaris-Cappio, F. (1986)Proc. Natl. Acad. Sci. U.S.A. 83, 2443–2447.

    Article  PubMed  CAS  Google Scholar 

  3. Camussi, G., Aglietta, M., Malavasi, F., Tetta, C., Piacibello, W., Sanavio, F., and Bussolino, F. (1983)J. Immunol. 131, 2397–2403.

    PubMed  CAS  Google Scholar 

  4. Prescott, S.M., Zimmerman, G.A., and McIntyre, T.M. (1984)Proc. Natl Acad. Sci. U.S.A. 81, 3534–3538.

    Article  PubMed  CAS  Google Scholar 

  5. Whatley, R.E., Nelson, P., Zimmerman, G.A., Stevens, D.L., Parker, C.J., McIntyre, T.M., and Prescott, S.M. (1989)J. Biol. Chem. 264, 6325–6333.

    PubMed  CAS  Google Scholar 

  6. Elstad, M.R., Prescott, S.M., McIntyre, T.M., and Zimmerman, G.A. (1988)J. Immunol. 140, 1618–1624.

    PubMed  CAS  Google Scholar 

  7. Snyder, F. (1985)Med. Res. Rev. 5, 107–140.

    Article  PubMed  CAS  Google Scholar 

  8. Hanahan, D.J. (1986)Ann. Rev. Biochem. 55, 483–509.

    Article  PubMed  CAS  Google Scholar 

  9. Prescott, S.M., Zimmerman, G.A., and McIntyre, T.M. (1987)Platelet-Activating Factor and Related Lipid Mediators, pp. 323–340, Plenum Press, New York.

    Google Scholar 

  10. Lenihan, D.J., and Lee, T-C. (1984)Biochem. Biophys. Res. Commun, 113, 51–58.

    Google Scholar 

  11. Elstad, M.R., Stafforini, D.M., McIntyre, T.M., Prescott, S.M., and Zimmerman, G.A. (1989)J. Biol. Chem. 264, 8467–8470.

    PubMed  CAS  Google Scholar 

  12. Blank, M.L., Lee, T-C., Fitzgerald, V., and Snyder, F. (1981)J. Biol. Chem. 256, 175–178.

    PubMed  CAS  Google Scholar 

  13. Farr, R.S., Cox, C.P., Wardlow, M.L., and Jorgensen, R. (1980)Clin. Immunol. Immunopathol. 15, 318–330.

    Article  PubMed  CAS  Google Scholar 

  14. Wardlow, M.L., Cox, C.P., Meng, K.E., Greene, D., and Farr, R.S. (1986)J. Immunol. 136, 3441–3446.

    PubMed  CAS  Google Scholar 

  15. Lee, T.-C., Malone, B., Wasserman, S.I., Fitzgerald, V., and Snyder, F. (1982)Biochem. Biophys. Res. Commun. 105, 1303–1308.

    Article  PubMed  CAS  Google Scholar 

  16. Stafforini, D.M., Prescott, S.M., and McIntyre, T.M. (1987)J. Biol. Chem. 262, 4223–4230.

    PubMed  CAS  Google Scholar 

  17. Stafforini, D.M., McIntyre, T.M., Carter, M.E., and Prescott, S.M. (1987)J. Biol. Chem. 262, 4215–4222.

    PubMed  CAS  Google Scholar 

  18. Blank, M.L., Hall, M.N., Cress, E.A., and Snyder, F. (1983)Biochem. Biophys. Res. Commun. 113, 666–671.

    Article  PubMed  CAS  Google Scholar 

  19. Nijssen, J.G., Roosenboom, C.F.P., and van den Bosch, H. (1986)Biochim. Biophys. Acta 876, 611–618.

    PubMed  CAS  Google Scholar 

  20. Yanoshita, R., Kudo, I., Ikizawa, K., Chang, H.W., Kobayashi, S., Ohno, M., Nojima, S., and Inoue, K. (1988)J. Biochem. 103, 815–819.

    PubMed  CAS  Google Scholar 

  21. Blackwell, G.J., Duncombe, W.G., Flower, R.J., Parsons, W.F., and Vane, J.R. (1977)Br. J. Pharmacol. 59, 353–366.

    PubMed  CAS  Google Scholar 

  22. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  23. Zimmerman, G.A., McIntyre, T.M., and Prescott, S.M. (1985)J. Clin. Invest. 76, 2235–2246.

    PubMed  CAS  Google Scholar 

  24. Davis, B.J. (1964)Ann. N.Y. Acad. Sci. 121, 404–427.

    Article  PubMed  CAS  Google Scholar 

  25. Brockerhoff, H., and Jensen, R.G. (1974) inLipolytic Enzymes, pp. 194–265, Academic Press, Orlando, FL.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah School of Medicine, 84112, Salt Lake City, Utah

    Diana M. Stafforini, Stephen M. Prescott, Guy A. Zimmerman & Thomas M. McIntyre

  2. Departments of Internal Medicine and Biochemistry, University of Utah School of Medicine, 84112, Salt Lake City, Utah

    Diana M. Stafforini, Stephen M. Prescott, Guy A. Zimmerman & Thomas M. McIntyre

Authors
  1. Diana M. Stafforini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Stephen M. Prescott
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Guy A. Zimmerman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Thomas M. McIntyre
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Stafforini, D.M., Prescott, S.M., Zimmerman, G.A. et al. Platelet-activating factor acetylhydrolase activity in human tissues and blood cells. Lipids 26, 979–985 (1991). https://doi.org/10.1007/BF02536488

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02536488

Keywords

Search

Navigation