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Effect of ethanol on platelet phospholipase A2

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Lipids

Abstract

Platelet aggregation is known to be inhibited by ethanol, and this has been suggested to be one of the attenuating effects of ethanol in cardiovascular disease. Recent studies have implicated an inhibition of phospholipase A2 induced arachidonic acid release, since the production of prostanoids that are formed from arachidonic acid and are involved in the aggregation process has been shown to be diminished by ethanol. Phospholipase A2 is found in platelets in both a cytosolic form, from where it may translocate to the plasma membrane to release arachidonic acid, and in a secretory form which is released extracellularly upon activition. In the present study, the effect of ethanol on the secretion of phospholipase A2 and on its activity was determined. It was found that ethanol inhibited trast, the activity of the cytosolic form of phospholipase A2 was inhibited by ethanol.

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Abbreviations

ATP:

adenosine triphosphate

C6-NBD-PC:

1-palmitoyl-2-N-(7-nitrobenzo-2-oxa-1,3-diazol-4-yl)amino)hexanoylglycerophosphocholine

EGTA:

ethyleneglycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid

HA:

hexanoic acid

HEPES:

N-[2-hydroxyethyl]-piperazine-N′-[2-ethanesulfonic acid]

References

  1. Stuart, M.J. (1979)J. Studies Alcohol 40, 1–6.

    CAS  Google Scholar 

  2. Pennington, S.N., and Smith, Jr., C.P. (1979)Prost. and Med. 2, 43–50.

    Article  CAS  Google Scholar 

  3. Haut, M., and Cowan, D. (1979)Am. J. Med. 56, 22–32.

    Article  Google Scholar 

  4. Hwang, D.H., LeBlanc, P., and Chanmugan, P. (1981)Lipids 16, 583–588.

    PubMed  CAS  Google Scholar 

  5. Hwang, D.H. (1981)Prost. and Med. 7, 511–513.

    Article  CAS  Google Scholar 

  6. Toivanen, J., Ylikorkala, O., and Viinikka, L. (1983)Thromb. Res. 33, 1–8.

    Article  Google Scholar 

  7. Segarnick, D.J., Ryer, H., and Rotrosen, J. (1985)Biochem. Pharm. 34, 1343–1346.

    Article  PubMed  CAS  Google Scholar 

  8. Pikaar, N.A., Wedel, M., van der Beek, E.J., van Dokkum, W., Kempen, H.J.M., Kluft, C., Ockhuizen, T., and Jermus, R.J.J. (1987)Metabolism 36, 538–543.

    Article  PubMed  CAS  Google Scholar 

  9. Barboriak, J.J. (1984)Alcohol 1, 341–345.

    Article  PubMed  CAS  Google Scholar 

  10. Halenda, S.P., and Rehm, A.G. (1987)Fed. Proc. 46, 398.

    Google Scholar 

  11. Silk, S.T., Clejan, S., and Witkom, K. (1989)J. Biol. Chem. 264, 21466–21469.

    PubMed  CAS  Google Scholar 

  12. Rubin, R. (1989)Arch. Biochem. Biophys. 270, 99–113.

    Article  PubMed  CAS  Google Scholar 

  13. Benistant, C., and Rubin, R. (1990)Biochem. J. 269, 489–497.

    PubMed  CAS  Google Scholar 

  14. Kramer, R.M., Johansen, B., Hession, C., and Pepinsky, R.B. (1990)Advances in Prostaglandin, Thromboxane, and Leukotriene Research 20, 79–86.

    PubMed  CAS  Google Scholar 

  15. van den Bosch, H., Aarsman, A.J., van Schaik, R.H.N., Schalkwijk, C.G., Neijs, F.W., and Sturk, A. (1990)Biochem. Soc. Trans. 18, 781–783.

    PubMed  Google Scholar 

  16. Purdon, A.D., Patelunas, A., and Smith, J.B. (1897)Biochim. Biophys. Acta 920, 205–214.

    Google Scholar 

  17. Kim, D.K., Kudo, I., Fujimori, Y., Mizushima, H., Masuda, M., Kikuchi, R., Ikizawa, K., and Inoue, K. (1990)J. Biochem. 108, 903–906.

    PubMed  CAS  Google Scholar 

  18. Schalkwijk, C.G., Marki, F., and van den Bosch, H. (1990)Biochim. Biophys. Acta 1044, 139–146.

    PubMed  CAS  Google Scholar 

  19. Takayama, K., Kudo, I., Kim, D.K., Nagata, K., Nozawa, Y., and Inoue, K. (1991)FEBS Lett. 282, 326–330.

    Article  PubMed  CAS  Google Scholar 

  20. Kim, D.Y., Kudo, I., and Inoue, K. (1991)Biochim. Biophys. Acta 1083, 80–88.

    PubMed  CAS  Google Scholar 

  21. Aarsman, A.J., Roosenboom, C.F.P., van Geffen, G.E.W., and van den Bosch, H. (1985)Biochim. Biophys. Acta 937, 288–295.

    Google Scholar 

  22. Horigome, K., Hawakawa, M., Inoue, K., and Nojima, S. (1987)J. Biochem. 101, 53–61.

    Article  PubMed  CAS  Google Scholar 

  23. Hayakawa, M., Kudo, I., Tomita, M., and Inoue, K. (1988)J. Biochem. 103, 263–266.

    PubMed  CAS  Google Scholar 

  24. Hayakawa, M., Kudo, I., Tomita, M., Nojima, S., and Inoue, K. (1988)J. Biochem. 104, 767–772.

    PubMed  CAS  Google Scholar 

  25. Aarsman, A.J., Leunissen-Bijvelt, J., van den Koedink, C.D.M.A., Neys, F.W., Verkleij, A.J., and van den Bosch, H. (1988)J. Lipid Med. 1, 49–61.

    Google Scholar 

  26. Kramer, R.M., Hession, C., Johansen, B., Hayes, G., McGray, P., Chow, P.E., Tizard, R., and Pepinsky, R.B. (1988)J. Biol. Chem. 264, 5768–5775.

    Google Scholar 

  27. Clark, J.D., Lin, L.L., Kriz, R.W., Ramesha, C.S., Sultzman, L.A., Lin, A.Y., Milona, N., and Knopf, J.L. (1991)Cell 65, 1043–1051.

    Article  PubMed  CAS  Google Scholar 

  28. Bills, T.K., Smith, J.B., and Silver, M.J. (1977)J. Clin. Invest. 60, 1–6.

    Article  PubMed  CAS  Google Scholar 

  29. Mahadevappa, V.G., and Holub, B.J. (1984)J. Biol. Chem. 259, 9369–9373.

    PubMed  CAS  Google Scholar 

  30. Holub, B.J. (1984)Can. J. Biochem. Cell Biol. 62, 341–351.

    Article  PubMed  CAS  Google Scholar 

  31. Colard, O., Breton, M., and Bereziat, G. (1987)Biochim. Biophys. Acta 921, 333–334.

    PubMed  CAS  Google Scholar 

  32. Kannagi, R., and Koizumi, K. (1979)Arch. Biochem. Biophys. 196, 534–542.

    Article  PubMed  CAS  Google Scholar 

  33. Derkson, A., and Cohen, P. (1975)J. Biol. Chem. 250, 9342–9347.

    Google Scholar 

  34. Bligh, E.G., and Dyer, W.J. (1959)Can. J. Biochem. Physiol. 37, 911–917.

    PubMed  CAS  Google Scholar 

  35. Stubbs, C.D., Williams, B.W., Pryor, C.L., and Rubin, E. (1988)Arch. Biochem. Biophys. 262, 560–573.

    Article  PubMed  CAS  Google Scholar 

  36. Conroy, D.C., Stubbs, C.D., Belin, J., Pryor, C.L., and Smith, A.D. (1986)Biochim. Biophys. Acta 861, 457–462.

    Article  PubMed  CAS  Google Scholar 

  37. Vitiello, F., and Zanetta, J.P. (1978)J. Chromatog. 166, 637–640.

    Article  CAS  Google Scholar 

  38. Pagano, R.E., and Sleight, R.G. (1985)Science, 229, 1051–1057.

    Article  PubMed  CAS  Google Scholar 

  39. Stubbs, C.D., Williams, B.W., Pryor, C.L., and Rubin, E. (1988)Arch. Biochem. Biophys. 262, 560–573.

    Article  PubMed  CAS  Google Scholar 

  40. Gerrard, J.M., Israels, S.J., and Friesen, L.L. (1985)Hematologie 27, 267–273.

    CAS  Google Scholar 

  41. Upreti, G.C., Rainier, S., and Jain, M.K. (1980)J. Membrane Biol. 55, 97–112.

    Article  CAS  Google Scholar 

  42. Burch, R.M., Luini, A., and Axelrod, J. (1986)Proc. Natl. Acad. Sci. USA 83, 7201–7205.

    Article  PubMed  CAS  Google Scholar 

  43. Jelsema, C.L. (1987)J. Biol. Chem. 262, 163–168.

    PubMed  CAS  Google Scholar 

  44. Halenda, S.P., and Rehm, A.G. (1987)Biochem. J. 248, 471–475.

    PubMed  CAS  Google Scholar 

  45. Nakashima, S., Hattori, H., Shirato, L., Tanaka, A., and Nozawa, Y. (1987)Biochem. Biophys. Res. Commun. 148, 971–978.

    Article  PubMed  CAS  Google Scholar 

  46. Nakashima, S., Tohmatsu, T., Hattori, H., Suganuma, A., and Nozawa, Y. (1987)J. Biochem. (Tokyo) 101, 1055–1058.

    CAS  Google Scholar 

  47. Rooney, T.A., Hager, R., Rubin, E., and Thomas, A.P. (1989)J. Biol. Chem. 264, 6817–6822.

    PubMed  CAS  Google Scholar 

  48. Halenda, S.P., Zavoico, G.B., and Sha'afi, R.I. (1985)J. Biol. Chem. 260, 12484–12491.

    PubMed  CAS  Google Scholar 

  49. Mobley, A., and Tai, H.H. (1985)Biochem. Biophys. Res. Commun. 130, 717–723.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology and Cell Biology, Thomas Jefferson University, Room 271 JAH, 1020 Locust Street, 19107, Philadelphia, Pennsylvania

    Christopher D. Stubbs & Raphael Rubin

Authors
  1. Christopher D. Stubbs
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  2. Raphael Rubin
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Stubbs, C.D., Rubin, R. Effect of ethanol on platelet phospholipase A2 . Lipids 27, 255–260 (1992). https://doi.org/10.1007/BF02536471

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  • DOI: https://doi.org/10.1007/BF02536471

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