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Injection of tridocosahexaenoyl-glycerol emulsion and fatty acid composition of blood cells

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Lipids

Abstract

An injectable emulsion of docosahexaenoic acid (DHA) was prepared. One hundred ml of the emulsion contained 3 g of 93%-pure 1,2,3-tridocosahexaenoylglycerol (DHA-TG), 1.2 g of 93%-pure 2-docosahexaenoyl-phosphatidylcholine as an emulsifier and 2.5 g of glycerol. Thirty ml of the emulsion of DHA-TG was injected into three rabbits on days 1 and 4 of the study. Blood was taken on day 0, on day 4 just before the second injection and on day 7. The percentage of DHA in the total phospholipid fraction of platelets increased from 0.46% (day 0) to 1.88% (day 4, p<0.05) and 3.66% (day 7; p<0.02 vs day 0). The percentage of eicosapentaenoic acid (EPA) increased from 0.46% (day 0) to 1.03% (day 4, p<0.02) and 1.63% (day 7: p<0.05 vs day 0). The percentage of arachidonic acid (AA) decreased from 9.45% (day 0) to 4.31% (day 4, p<0.05) and 6.68% (day 7; p<0.02 vs day 0). The percentage of DHA in the total phospholipid fraction of erythrocyte membranes increased from 0.23% (day 0) to 0.91% (day 4, p<0.05) and 1.52% (day 7; p<0.005 vs day 0); that of EPA increased from 0.21% (day 0) to 0.34% (day 4, p<0.005%) and 0.52% (day 7, p<0.01 vs day 0); that of AA was unchanged. Blood lipids were the same before and after the two injections of the emulsion, except that free fatty acids decreased markedly from 0.32 to 0.06 mEq/1 (p<0.02). On day 8, free AA (2 mg/kg) was injected into ear veins of the three treated rabbits and of four control rabbits (not treated with DHA-TG). All the control rabbits died a few minutes after the AA injection, but none of the DHA-treated rabbits died after AA injection (p<0.01). An emulsion of DHA-TG may be useful for patients having immediate risk of thrombosis or for those who need DHA but cannot take it orally.

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Abbreviations

DHA:

docosahexaenoic acid

DHA-TG:

1,2,3-tridocosahexaenoyl-glycerol

AA:

arachidonic acid

DHA-PC:

2-docosahexaenoyl-phosphatidylcholine

PRP:

platelet-rich plasma

EPA:

eicosapentaenoic acid

RBC:

red blood cell

EPA-TG:

1,2,3-trieicosapentaenoyl-glycerol

References

  1. Holman, R.T. (1976) inLipids (Paoletti, R., Porcellatti, G., and Jacini, G., eds.) Vol. 1, pp 215–226, Raven Press, New York.

    Google Scholar 

  2. Walker, B.L. (1967)Lipids 2, 497–500.

    Article  CAS  PubMed  Google Scholar 

  3. Holma, R.T., Johnson, S.B., and Hatch, T.F. (1982)Am. J. Clin. Nutr. 35, 617–623.

    Google Scholar 

  4. Neuringer, M., Connor, W.E., Petten, C.V., and Barstad, L. (1984)J. Clin. Invest. 73, 272–276.

    PubMed  CAS  Google Scholar 

  5. Neuringer, M., Connor, W.E., Lin, D.S., Barstad, L., and Luck, S. (1986)Proc. Natl. Acad. Sci. USA 83, 4021–4025.

    Article  PubMed  CAS  Google Scholar 

  6. Svennerholm, L. (1968)J. Lipid Res. 9, 570–579.

    PubMed  CAS  Google Scholar 

  7. Anderson, R.E. (1970)Exp. Eye Res. 10, 339–344.

    Article  PubMed  CAS  Google Scholar 

  8. Dyerberg, J., Bang, H.O., and Aagaard, O. (1980)Lancet 1, 199.

    Article  PubMed  CAS  Google Scholar 

  9. McClead, R.E. Jr., Meng, H.C., Gregory, S.A., Budde, C., and Sloan, H.R. (1985)J. Pediatr. Gastr. Nutr. 4, 234–239.

    Article  Google Scholar 

  10. Hamazaki, T., Hirai, A., Terano, T., Sajiki, J., Kondo, S., Fujita, T., Tamura, Y., and Kumagai, A. (1982)Prostaglandins 23, 557–567.

    Article  PubMed  CAS  Google Scholar 

  11. Diczfalusy, U., and Hamberg, M. (1986)Biochim. Biophys. Acta 878, 387–393.

    PubMed  CAS  Google Scholar 

  12. Garton, G.A., and Dunkan, W.R.H. (1957)Biochem. J. 67, 340–345.

    PubMed  CAS  Google Scholar 

  13. Geyer, R.P., Olsen, F.R., Andrus, S.B., Waddell, W.R., and Stare, F.J. (1955)J. Am. Oil Chem. Soc. 32, 365–370.

    CAS  Google Scholar 

  14. Vorbeck, M.L., Mattick, L.R., Lee, F.A., and Pederson, C.S. (1961)Anal. Chem. 33, 1512–1514.

    Article  CAS  Google Scholar 

  15. Allain, C.C., Poon, L.S., Chan, C.S.G., Richmond, W., and Fu, P.C. (1974)Clin. Chem. 20, 470–475.

    PubMed  CAS  Google Scholar 

  16. Uwajima, T., Akita, H., Ito, K., Mihara, A., Aisaka, K., and Terada, O. (1980)Agric. Biol. Chem. 44, 399–406.

    CAS  Google Scholar 

  17. Kawaguchi, A., Hosaka, K., Kikuchi, T., Soda, K., and Okuda, S. (1983)J. Biochem. 94, 487–492.

    PubMed  CAS  Google Scholar 

  18. Harris, W.S., Connor, W.E., and Lindsey, S. (1984)Am. J. Clin. Nutr. 40, 780–785.

    PubMed  CAS  Google Scholar 

  19. Innis, S.M. (1986)Lipids 21, 132–138.

    Article  PubMed  CAS  Google Scholar 

  20. Urakaze, M., Hamazaki, T., Soda, Y., Miyamato, A., Ibuki, F., Yano, S., and Kumagai, A. (1986)Thromb. Res. 44, 673–682.

    Article  PubMed  CAS  Google Scholar 

  21. von Schacky, C., and Weber, P.C. (1985)J. Clin. Invest. 76, 2446–2450.

    Article  Google Scholar 

  22. Goodnight, S.H. Jr., Harris, W.S., Connor, W.E., and Illingworth, D.R. (1982)Arteriosclerosis 2, 87–113.

    PubMed  CAS  Google Scholar 

  23. Iritani, N., Inoguchi, K., Endo, M., Fukuda, E., and Morita, M. (1980)Biochim. Biophys. Acta 618, 378–382.

    PubMed  CAS  Google Scholar 

  24. Morisaki, N., Shinomiya, M., Matsuoka, N., Saito, Y., and Kumagai, A. (1983)Tohoku J. Exp. Med. 141, 397–405.

    Article  PubMed  CAS  Google Scholar 

  25. Talesnik, J. (1986)Can. J. Physiol. Pharmacol. 64, 77–84.

    PubMed  CAS  Google Scholar 

  26. Lands, W.E.M., LeTellier, P.R., Rome, L.H., and Vanderhoek, J.Y. (1973) inAdvances in the Biosciences (Bergstrom, S., and Bernhard, S., eds.) Vol. 9, pp. 15–28, Pergamon Press, Vieweg, Braunschweig.

    Google Scholar 

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Authors and Affiliations

  1. The First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, Sugitani, 930-01, Toyama City, Toyama, Japan

    Tomohito Hamazaki, Masaharu Urakaze & Saburo Yano

  2. Medical and Pharmaceutical Laboratory, Nippon Shoji Kaisha Ltd., Ibaraki City, Osaka, Japan

    Yasuji Soda & Akira Miyamoto

  3. General Technical Department, Nippon Oil and Fats Co. Ltd., Chiyoda-ku, Tokyo, Japan

    Kohji Kubota

  4. The First Division, Research and Development, Daigo Nutritive Chemicals Ltd., Yodogawa-Ku, Osaka, Japan

    Fumio Ibuki

Authors
  1. Tomohito Hamazaki
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  2. Masaharu Urakaze
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  3. Saburo Yano
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  4. Yasuji Soda
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  5. Akira Miyamoto
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  6. Kohji Kubota
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  7. Fumio Ibuki
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Hamazaki, T., Urakaze, M., Yano, S. et al. Injection of tridocosahexaenoyl-glycerol emulsion and fatty acid composition of blood cells. Lipids 22, 1031–1034 (1987). https://doi.org/10.1007/BF02536445

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  • DOI: https://doi.org/10.1007/BF02536445

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