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Free and combined sterols ofPavlova gyrans

  • Plant and Fungal Sterols: Biosynthesis, Metabolism and Function Papers Presented at a Symposium Held at the AOCS Annual Meeting. Baltimore, MD, April 1990
  • Article
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Lipids

Abstract

Pavlova gyrans, a unicellular alga of interest as food for oysters, was cultured axenically and examined for sterol composition. Desmethyl monohydroxy sterols, which are frequently seen in algae, made up 40% of the total sterols and were observed primarily in the free sterol fraction. The principal sterols of this group were 5-ergostenol, poriferasterol, and clionasterol, as well as some poriferast-22-enol and poriferastanol. Several “methyl” sterols with unusual structures made up 27% of the total sterols. The principal “methyl sterols” were 4α-methyl ergostanol, 4α-methyl poriferastanol, and 4α-methyl poriferast-22-enol. Methyl sterols were found primarily in the ester fraction. Also observed was a new class of dihydroxysterols composing 33% of the total sterols. These sterols are structurally related to the methyl and desmethyl sterols ofPavlova but contain an extra nuclear hydroxyl which can be acetylated when present on a desmethyl sterol, but which is nonreactive with acetic anhydride in 4α-methyl sterols. None of these sterols were observed in ester form but are concentrated in the acid-hydrolyzable, bound fraction. The unique nature of these sterols suggests potential taxonomic utility.

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Abbreviations

GC:

gas chromatography

GLC:

gas-liquid chromatography

NMR:

nuclear magnetic resonance

RRT:

relative retention times

References

  1. Lin, D.S., Ilias, A.M., Connor, W.E., Caldwell, R.S., Cory, H.T., and Davies, Jr., G.D. (1982)Lipids 17, 818–824.

    CAS  Google Scholar 

  2. Berenberg, C.J., and Patterson, G.W. (1981)Lipids 16, 276–278.

    CAS  Google Scholar 

  3. Teshima, S., and Patterson, G.W. (1980)Lipids 15, 1004–1011.

    Article  CAS  Google Scholar 

  4. Teshima, S., and Patterson, G.W. (1981)Lipids 16, 234–239.

    CAS  Google Scholar 

  5. Holden, M.J., and Patterson, G.W. (1991)Lipids 26, 81–82.

    CAS  Google Scholar 

  6. Patterson, G.W. (1971)Lipids 6, 120–127.

    Article  CAS  Google Scholar 

  7. Raederstorff, D., and Rohmer, M. (1984)Phytochemistry 23, 2835–2838.

    Article  CAS  Google Scholar 

  8. Maxwell, J.R., Mackenzie, A.S., and Volkman J.K. (1980)Nature 286, 694–697.

    Article  CAS  Google Scholar 

  9. Cristensen, T. (1966) inSystematisk Botanik (Bocher, T.W., Lange, M., and Sorensen, T. eds.) 2nd edn., Munksgard, Copenhagen.

    Google Scholar 

  10. Hibberd, D.J. (1976)Bot. J. Linn. Soc. 72, 55–80.

    Google Scholar 

  11. Walne, P.R. (1970)Ministry of Agriculture, Fisheries and Food, Fishery Investigations, Ser. II, Vol. XXVI, No. 5, Her Majesty's Stationery Office, London.

    Google Scholar 

  12. Ballantine, J.A., Lavis, S. and Morris, R.J. (1979)Phytochemistry 18, 1459–1466.

    Article  CAS  Google Scholar 

  13. Ukeles, R. (1973)Handbook of Phycological Methods—Culture Methods and Growth Measurements (Stein, J., ed.) pp. 233–254, Cambridge University Press, London.

    Google Scholar 

  14. Gershengorn, M.C., Smith, A.R.H., Goulston, G., Goad, L.J., Goodwin, T.W., and Haines, T.H. (1968)Biochemistry 7, 1698–1706.

    Article  PubMed  CAS  Google Scholar 

  15. Cranwell, P.A., Creighton, M.E., and Jaworski, G.H.M. (1988)Phytochemistry 27, 1053–1059.

    Article  CAS  Google Scholar 

  16. Thompson, M.J., Dutky, S.R., Patterson, G.W., and Gooden, E.L. (1972)Phytochemistry 11, 1781–1790.

    Article  CAS  Google Scholar 

  17. Rubinstein, I., Goad, L.J., Clague, D.H., and Mulheirn, L.J. (1976)Phytochemistry 15, 195–200.

    Article  CAS  Google Scholar 

  18. Mulheirn, L.J. (1973)Tetrahedron Lett., 3175–3178.

  19. Goad, L.J., Holtz, G.G., and Beach, D.H. (1983)Phytochemistry 22, 475–476.

    Article  CAS  Google Scholar 

  20. Teshima, S., and Patterson, G.W. (1981)Comp. Biochem. Physiol. 69B, 175–181.

    CAS  Google Scholar 

  21. Kokke, W.C.M.C., Bohlin, L., Fenical, W., and Djerassi, C. (1982)Phytochemistry 21, 881–887.

    Article  CAS  Google Scholar 

  22. Alam, M., Sanduja, R., Watson, D.A., and Loeblich, III, A.R. (1984)J. Phycol. 20, 331–335.

    Article  CAS  Google Scholar 

  23. Alam, M., Sansing, T.B., Guerra, J.R., and Harmon, A.D. (1981)Steroids 38, 375–382.

    Article  PubMed  CAS  Google Scholar 

  24. Kokke, W.C.M.C., Fenical, W., and Djerassi., C. (1981)Phytochemistry 20, 127–134.

    Article  CAS  Google Scholar 

  25. Chardon-Loriaux, I., Morisaki, M., and Ikekawa, N. (1976)Phytochemistry 15, 723–725.

    Article  CAS  Google Scholar 

  26. Wakeham, S.G. (1989)Nature 342, 787–790.

    Article  CAS  Google Scholar 

  27. Xu, S., and Patterson, G.W. (1990)Lipids 25, 230–234.

    CAS  Google Scholar 

  28. Patterson, G.W., and Xu, S. (1990)Phytochemistry 29, 3539–3541.

    Article  CAS  Google Scholar 

  29. Volkman, J.K., Smith, D.J., Eglington, G., Forsberg, T.E.V., and Corner, E.D.S. (1981)J. Mar. Biol. Assoc. 61, 509–527.

    Article  CAS  Google Scholar 

  30. Marlowe, I.T., Green, J.C., Neal, A.C., Brassell, S.C., Eglington, G., and Course, P.A. (1984)Br. Phycol. J. 19, 203–216.

    Google Scholar 

  31. Wright, D.C., Berg., L.R., and Patterson, G.W. (1980)Phytochemistry 19, 783–785.

    Article  CAS  Google Scholar 

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Author information

Author notes

  1. Patricia K. Gladu

    Present address: Biology Department, Lindsey-Wilson College, 42728, Columbia, Kentucky

Authors and Affiliations

  1. Department of Botany, University of Maryland, 20742, College Park, Maryland

    Patricia K. Gladu & Glenn W. Patterson

  2. National Marine Fisheries Service, Northeast Fisheries Center, Milford Laboratory, NOAA, 06460, Milford, Connecticut

    Gary H. Wikfors

  3. Insect Physiology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 20705, Beltsville, Maryland

    William R. Lusby

Authors
  1. Patricia K. Gladu
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  2. Glenn W. Patterson
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  3. Gary H. Wikfors
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  4. William R. Lusby
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Gladu, P.K., Patterson, G.W., Wikfors, G.H. et al. Free and combined sterols ofPavlova gyrans . Lipids 26, 656–659 (1991). https://doi.org/10.1007/BF02536431

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  • DOI: https://doi.org/10.1007/BF02536431

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