Abstract
Studies were performed to examine the effect of a lard diet on tumorigenesis by 7,12-dimethylbenzanthracene (DMBA), given parenterally rather than by gavage, to eliminate any effect of the high lard diet on carcinogen absorption. In addition, the effect of low dietary levels of the antioxidants butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) in the tumor model was evaluated. The lards fed were analyzed for fatty acid composition and content of certain potential contaminants. DMBA induced tumors when given by intravenous or subcutaneous injection. The high lard diet appeared to enhance tumorigenesis in rats given a dose of 0.25 mg (10% of the gavage dose) by injection into the mammary gland, although the effect was not statistically significant. In other experiments using lard from different sources and DMBA given by gavage, significant enhancement of tumorigenesis was limited to groups fed the high lard diets throughout the experiment or beginning after DMBA exposure. In contrast to earlier results, there was no demonstrable effect of feeding the high lard diets before DMBA administration. Addition of BHA and BHT to the lard at the concentration assayed in commercial lard samples or at the maximum concentration permitted did not influence the tumorigenesis. In groups in which tumorigenesis was enhanced by the high lard diet, the incidence of malignant, invasive tumors was higher than in other groups.
Similar content being viewed by others
References
Carroll, K.K. (1980)J. Environ. Pathol. Toxicol. 3, 253–271.
Braden, L.M., and Carroll, K.K.,Lipids 21, 285–288.
National Academy of Science-National Research Council (1982)Diet, Nutrition and Cancer, National Academy Press, Washington, DC.
Hopkins, G.J., and Carroll, K.K. (1979)JNCI 62, 1009–1012
Wetsel, W.C., Rogers, A.E., and Newberne, P.M. (1981)Cancer Detection and Prevention 4, 535–543.
Rogers, A.E. (1983)Cancer Res. 43, 2477s-2484s.
Chang, P.C., Ferguson, K.A., and Dao, T.L. (1983).Cancer Res. 43, 1079–1083.
Selenskas, S.L., Ip, M.M., and Ip, C. (1984)Cancer Res. 44, 1321–1326.
Dao, T.L., and Chan, P.C. (1983)JNCI 71, 201–205.
Kritchevsky, D., Weber, M.M., and Klurfeld, D.M. (1984)Cancer Res. 44, 3174–3177.
Rogers, A.E., Conner, B.H., Boulanger, C., Lee, S.Y., Carr, F.A., and DuMouchel, W. (1985) inBasic and Clinical Aspects of Dietary Fiber (Vahouny, G., and Kritchevsky, D., eds.) pp. 449–455, Plenum Press, New York.
King, M.M., and McCay, P.B. (1983)Cancer Res. 43, 2485s-2490s.
McCormick, D.L., Major, N., and Moon, R.C. (1984)Cancer Res. 44, 2858–2863.
Cohen, L.A., Polansky, M., Furuya, K., Reddy, M., Berke, B., and Weisburger, J.H. (1984)JNCI 72, 165–174.
Greaves, P., and Faccini, J.M. (1984)Rat Histopathology, Elsevier, Amsterdam, pp. 10–14.
Young, S., and Hollowes, R.C. (1973) inPathology of Tumors in Laboratory Animals: Rats (Turosov, E.V. ed.) Vol. I, IARC, Lyon.
BMDP 79 (1979) inBiomedical Computer Programs P-Series (Dixon, W.J., and Brown, M.B. eds.) University of California Press, Berkeley.
Sylvester, P.W., Russell, M., Ip, M.M., and Ip, C. (1985)Am. Assoc. for Cancer Res. Proc. 26, 126.
Carroll, K.K., Khor, H.T. (1971)Lipids, 6, 415–420.
Author information
Authors and Affiliations
About this article
Cite this article
Rogers, A.E., Conner, B., Boulanger, C. et al. Mammary tumorigenesis in rats fed diets high in lard. Lipids 21, 275–280 (1986). https://doi.org/10.1007/BF02536412
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02536412