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Effect of 25-hydroxycholesterol on cholesteryl ester formation in Caco-2 cells

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Lipids

Abstract

Incubation of Caco-2 cells, a human intestinal cell line, with 25-hydroxycholesterol (25-HOC) markedly enhanced cellular cholesteryl ester formation determined by incorporation of [14C]oleic acid into intracellular cholesteryl [14C]oleate. The stimulation by 25-HOC of cholesteryl ester formation was suppressed by staurosporine, a kinase inhibitor, but not by cycloheximide or actinomycin D. The specific activity of microsomal acyl-coenzyme A:cholesterol acyltransferase (ACAT) increased two-fold in cells treated with 10 μM 25-HOC for 5 h. ACAT activity decreased when microsomes were incubated without sodium fluoride, a phosphatase inhibitor, but the decrease in ACAT activity in cells stimulated with 25-HOC was more pronounced. The results suggest that protein phosphorylation may be involved in the stimulation of cholesteryl ester formation by 25-HOC in Caco-2 cells.

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Abbreviations

ACAT:

acyl-coenzyme A:cholesterol acyltransferase

AD:

actinomycin D

BSA:

bovine serum albumin

CHX:

cycloheximide

DMEM:

Dulbecco's modified Eagle medium

25-HOC:

25-hydroxycholesterol

STP:

staurosporine

TLC:

thin-layer chromatography

References

  1. Goldstein, J.L., and Brown, M.S. (1990)Nature 343, 425–430.

    Article  PubMed  CAS  Google Scholar 

  2. Brown, M.S., Dana, S.E., and Goldstein, J.L. (1975)J. Biol. Chem. 250, 425–430.

    Google Scholar 

  3. Drevon, C.A., Weinstein, D.B., and Steinberg, D. (1980)J. Biol. Chem. 255, 9128–9137.

    PubMed  CAS  Google Scholar 

  4. Field, F.J., and Mathur, S.N. (1983)J. Lipid Res. 24, 1049–1059.

    PubMed  CAS  Google Scholar 

  5. Brown, M.S., Ho, Y.K., and Goldstein, J.L. (1980)J. Biol. Chem. 255, 9344–9352.

    PubMed  CAS  Google Scholar 

  6. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  7. Suckling, K.E., Stange, E.F., and Dietschy, J.M. (1983)FEBS Lett. 151, 111–116.

    Article  PubMed  CAS  Google Scholar 

  8. Gavey, K.L., Trujillo, D., and Scallen, T.J. (1983)Proc. Natl. Acad. Sci. USA 80, 2171–2174.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Laboratories, Yoshitomi Pharmaceutical Industries, Ltd., 7-25, Koyata 3-Chome Iruma-Shi, 358, Saitama, Japan

    Hidenobu Kusuhara, Osafumi Shimada & Jun Inui

Authors
  1. Hidenobu Kusuhara
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  2. Osafumi Shimada
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  3. Jun Inui
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Kusuhara, H., Shimada, O. & Inui, J. Effect of 25-hydroxycholesterol on cholesteryl ester formation in Caco-2 cells. Lipids 27, 478–480 (1992). https://doi.org/10.1007/BF02536393

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  • DOI: https://doi.org/10.1007/BF02536393

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