Skip to main content

Advertisement

SpringerLink
Log in

Induction of long-lasting hypercholesterolemia in the rat fed a cystine-enriched diet

  • Published:
Lipids

Abstract

The influence of dietary excess (5%) of L-cystine on rat plasma lipoproteins was examined. After only one week of cystine feeding, an increase in the plasma cholesterol level and a decrease in triglyceride levels were observed. The increase in cholesterol level became greater when the duration of cystine-enriched diet increased until eight weeks (+131% after eight weeks), but no further increase occurred between 8 and 20 weeks. This change was essentially due to the progressive increase in cholesterol levels in high density lipoproteins (HDL) and in lipoproteins isolated between 1.040 and 1.063 g/ml, i.e., certain low density lipoproteins (HDL2), and containing mainly apoE-rich lipoproteins (HDL1). The decrease in plasma triglycerides resulted from that of chylomicrons and very low density lipoproteins (VLDL). The effects observed after four or eight weeks of cystine feeding were maintained for eight weeks after replacing the cystine diet by the standard diet. Ingestion of the standard diet containing either cholestyramine (2%) or probucol (0.25%) following eight weeks of cystine feeding significantly decreased plasma cholesterol levels. It is concluded that cystine-fed rats are a useful tool of investigation for understanding mechanisms leading to increased plasma cholesterol level and for hypocholesterolemic drug trials.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CETP:

cholesteryl ester transfer protein

EDTA:

ethylenediamine tetraacetic acid

HDL:

high density lipoprotein

LCAT:

lecithin: cholesterol acyl transferase

(V)LDL:

(very) low density lipoprotein

MIA:

monoiodoacetamide

PTU:

propylthiouracil

References

  1. Lasser, N.L., Roheim, P.S., Edelstein, D., and Eder, H.A. (1973)J. Lipid Res. 14, 1–8.

    PubMed  CAS  Google Scholar 

  2. Mahley, R.W., and Holcombe, K.S. (1977)J. Lipid Res. 18, 314–324.

    PubMed  CAS  Google Scholar 

  3. Noel, S.P., Wong, L., Dolphin, P.J., Dory, L., and Rubinstein, D. (1979)J. Clin. Invest. 64, 674–683.

    PubMed  CAS  Google Scholar 

  4. Swift, L.L., Manowitz, N.R., Dunn, G.D., and Lequire, V.S. (1980)J. Clin. Invest. 66, 415–425.

    Article  PubMed  CAS  Google Scholar 

  5. Delamatre, J.G., Roheim, P.S. (1981)J. Lipid Res. 22, 297–306.

    PubMed  CAS  Google Scholar 

  6. Dolphin, P.J. (1981)J. Lipid Res. 22, 971–989.

    PubMed  CAS  Google Scholar 

  7. Goldstein, J.L., and Brown, M.S. (1983) inThe Metabolic Basis of Inherited Disease (Standbury, J.B., Wyngaarden, J.B., Fredrickson, D.S., Goldstein, J.L., and Brown, M.S., eds.) pp. 672–712, McGraw-Hill Book Company, New York, NY.

    Google Scholar 

  8. Sérougne, C., and Rukaj, A. (1983)Ann. Nutr. Metab. 27, 386–395.

    PubMed  Google Scholar 

  9. Sérougne, C., Férézou, J., and Rukaj, A. (1984)Ann. Nutr. Metab. 28, 311–320.

    Article  PubMed  Google Scholar 

  10. Mathé, D., Lutton, C., Rautureau, J., Coste, T., Gouffier, E., Sulpice, J.-C., and Chevallier, F. (1977)J. Nutr. 107, 466–474.

    PubMed  Google Scholar 

  11. Terpstra, A.H.M., Woodward, C.J.H., and Sanchez-Muniz, F.J. (1981)Anal. Biochem. 111, 149–157.

    Article  PubMed  CAS  Google Scholar 

  12. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  13. Connelly, P.W., and Kuksis, A. (1982)Biochim. Biophys. Acta 711, 245–251.

    PubMed  CAS  Google Scholar 

  14. Weber, K., and Osborn, M. (1969)J. Biol. Chem. 244, 4406–4412.

    PubMed  CAS  Google Scholar 

  15. Oschry, Y., and Eisenberg, S. (1982)J. Lipid Res. 23, 1099–1106.

    PubMed  CAS  Google Scholar 

  16. Carroll, K.K. (1978)Nutr. Rev. 36, 1–5.

    Article  PubMed  CAS  Google Scholar 

  17. Kritchevsky, D. (1979)J. Am. Oil Chem. Soc. 56, 135–140.

    PubMed  CAS  Google Scholar 

  18. Yadav, N.R., and Liener, I.E. (1977)Nutr. Rep. Int. 16, 385–389.

    CAS  Google Scholar 

  19. Nagata, Y., Tanaka, K., and Sugano, M. (1981)Br. J. Nutr. 45, 233–241.

    Article  PubMed  CAS  Google Scholar 

  20. Terpstra, A.H.M., Van Tintelen, G., and West, C.E. (1982)J. Nutr. 112, 810–817.

    PubMed  CAS  Google Scholar 

  21. Sirtori, C.R., Gatti, E., Mantero, O., Conti, F., Agradi, E., Tremoli, E., Sirtori, M., Fraterrigo, L., Tavazzi, L., and Kritchevsky, D. (1979)Am. J. Clin. Nutr. 32, 1645–1658.

    PubMed  CAS  Google Scholar 

  22. Wolfe, B., Giovanetti, P., Cheng, D.C.H., Roberts, D.C.K., and Carroll, K.K. (1981)Nutr. Rep. Int. 24, 1187–1198.

    CAS  Google Scholar 

  23. Goldberg, A.P., Lin, A., Kolar, J.B., Grundhauser, J.J., Steinke, F.H., and Schonfeld, G. (1982)Atherosclerosis 43, 355–358.

    Article  PubMed  CAS  Google Scholar 

  24. Huff, M.W., Hamilton, R.M.G., and Carroll, K.K. (1977)Atherosclerosis 28, 187–195.

    Article  PubMed  CAS  Google Scholar 

  25. Sugano, M. (1983) inAnimal and Vegetable Proteins in Lipid Metabolism and Atherosclerosis (Gibney, M.J., and Kritchevsky, D., eds.), pp. 51–81, Alan R. Liss Inc., New York, NY.

    Google Scholar 

  26. Sérougne, C., Férézou, J., and Rukaj, A. (1987)Biochim. Biophys. Acta 921, 522–530.

    PubMed  Google Scholar 

  27. Lusk, L.T., Walker, L.F., DuBien, L.H., and Getz, G.S. (1979)Biochem. J. 183, 83–90.

    PubMed  CAS  Google Scholar 

  28. Fidge, N.H., and Poulis, P. (1978)J. Lipid Res. 19, 342–349.

    PubMed  CAS  Google Scholar 

  29. Weisgraber, K.H., and Mahley, R.W. (1980)J. Lipid Res. 21, 316–325.

    PubMed  CAS  Google Scholar 

  30. Gordon, V., Innerarity, T.L., and Mahley, R.W. (1983)J. Biol. Chem. 258, 6202–6212.

    PubMed  CAS  Google Scholar 

  31. Koo, C., Innerarity, T.L., and Mahley, R.W. (1985)J. Biol. Chem. 260, 11934–11943.

    PubMed  CAS  Google Scholar 

  32. Dory, L., and Roheim, P.S. (1981)J. Lipid Res. 22, 287–296.

    PubMed  CAS  Google Scholar 

  33. Lefevre, M., Keen, C.L., Lönnerdal, B., Herley, L.S., and Schneeman, B.O. (1986)J. Nutr. 116, 1735–1746.

    PubMed  CAS  Google Scholar 

  34. Lowe, K.E., Pelkey, S., Williams, M.A., and Nichols, A.V. (1988)Lipids 23, 106–114.

    PubMed  CAS  Google Scholar 

  35. Koga, S., Miyata, Y., Ibayashi, H. (1985)Hepatology 5, 286–292.

    PubMed  CAS  Google Scholar 

  36. Mitchell, C.D., King, W.C., Applegate, K.R., Forte, T., Glomset, J.A., Norum, K.R., and Gjone, E. (1980)J. Lipid Res. 21, 625–634.

    PubMed  CAS  Google Scholar 

  37. Weidman, S.W., Ragland, J.B., and Sabesin, S.M. (1982)J. Lipid Res. 23, 556–569.

    PubMed  CAS  Google Scholar 

  38. Tall, A.R., and Small, J.M. (1978)New Engl. J. Med. 299, 1232–1236.

    Article  PubMed  CAS  Google Scholar 

  39. Eisenberg, S. (1984)J. Lipid Res. 25, 1017–1058.

    PubMed  CAS  Google Scholar 

  40. Gavish, D., Oschry, Y., and Eisenberg, S. (1987)J. Lipid Res. 28, 257–267.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Physiologie de la Nutrition-Bâtiment 447, CNRS UA 646, Université de Paris-Sud, 91405, ORSAY Cedex, France

    C. Sérougne & C. Lutton

  2. Unité de Recherches sur les dyslipidémies et l'athérosclérose (U 32), Hopital Henri Mondor, 94010, CRETEIL, France

    D. Mathé

Authors
  1. C. Sérougne
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Mathé
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Lutton
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Sérougne, C., Mathé, D. & Lutton, C. Induction of long-lasting hypercholesterolemia in the rat fed a cystine-enriched diet. Lipids 23, 930–936 (1988). https://doi.org/10.1007/BF02536339

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02536339

Keywords

Search

Navigation