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Phospholipid analysis of human eosinophils: High levels of alkylacylglycerophosphocholine (PAF precursor)

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Lipids

Abstract

The phospholipid composition and fatty acid profiles of human eosinophils were studied. Extremely high levels of ether phospholipids were found in this type of cell, such as alkylacylglycerophosphocholine (GPC) and alkenyl-acylglycerophosphoethanolamine (GPE); these two ether phospholipids accounted for about three-fourths of the choline and ethanolamine glycerophospholipids (CGP and EGP), respectively. Fatty acid analyses revealed that very large portions of arachidonic acid (20∶4) were esterified to alkylacyl-GPC (92.0% in CGP) and alkenylacyl-GPE (86.6% in EGP), respectively. While high amounts of alkylacyl-GPC and the abundance of 20∶4 in this ether phospholipid have been observed in other types of blood cells of various animals, these percentages for human eosinophils are the highest among such cells. These results suggest that alkyl and alkenyl ether phospholipids play essential roles in human eosinophils in various physiological and pathological conditions.

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Abbreviations

CGP:

choline glycerophospholipids

ECF-A:

eosinophil chemotactic factor of anaphylaxis

EDTA:

ethylenediamine tetraacetic acid

EGP:

ethanolamine glycerophospholipids

FMLP:

formylmethionylleucylphenylalanine

GPC:

glycerophosphocholine

PAF:

platelet-activating factor

GLC:

gas liquid chromatography

TLC:

thin layer chromatography

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Tsukui-gun, 199-01, Kanagawa, Japan

    Ayako Ojima-Uchiyama, Yasuo Masuzawa, Takayuki Sugiura & Keizo Waku

  2. Research Center for Rheumato-Allergology, National Sagamihara Hospital, 228, Sagamihara City Kanagawa, Japan

    Hiroshi Saito & Yasuo Yui

  3. School of Medicine, Chiba University, 280, Chiba City, Chiba, Japan

    Hisao Tomioka

Authors
  1. Ayako Ojima-Uchiyama
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  2. Yasuo Masuzawa
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  3. Takayuki Sugiura
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  4. Keizo Waku
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  5. Hiroshi Saito
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  6. Yasuo Yui
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  7. Hisao Tomioka
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Ojima-Uchiyama, A., Masuzawa, Y., Sugiura, T. et al. Phospholipid analysis of human eosinophils: High levels of alkylacylglycerophosphocholine (PAF precursor). Lipids 23, 815–817 (1988). https://doi.org/10.1007/BF02536227

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  • DOI: https://doi.org/10.1007/BF02536227

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