Abstract
Oral administration of a single dose of tri- or hexadeuterium substituted 2R,4′R,8′R-α-tocopheryl acetate (d3- or d6-α-T-Ac) to humans was used to follow the absorption and transport of vitamin E in plasma lipoproteins. Three hr after oral administration of d3-α-T-Ac (15 mg) to 2 subjects, plasma levels of d3-α-T were detectable; these increased up to 10 hr, reached a plateau at 24 hr, then decreased. Following administration of d6-α-T-Ac (15–16 mg) to 2 subjects, the percentage of deuterated tocopherol relative to the total tocopherol in chylomicrons increased more rapidly than the corresponding percentage in whole plasma. Chylomicrons and plasma lipoproteins were isolated from 2 additional subjects following administration of d3-α-T-Ac (140 or 60 mg). The percentage of deuterated tocopherol relative to the total tocopherol increased most rapidly in chylomicrons, then in very low density lipoproteins (VLDL), followed by essentially identical increases in low and high density lipoproteins (LDL and HDL, respectively) and lastly, in the red blood cells. This pattern of appearance of deuterated tocopherol is consistent with the concept that newly absorbed vitamin E is secreted by the intestine into chylomicrons; subsequently, chylomicron remnants are taken up by the liver from which the vitamin E is secreted in VLDL. The metabolism of VLDL in the circulation results in the simultaneous delivery of vitamin E into LDL and HDL.
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Abbreviations
- d0-α-T:
-
nondéuterated α-tocopherol
- d3-α-T:
-
2R,4′R,8′R-α-(5-C2H3)tocopherol
- d6-α-T:
-
2R,4′R,8′R-α-(5,7-[C2H3]2)tocopherol
- d9-α-T:
-
ambo-α-(5,7,8-[C2H3]3)tocopherol
- d3-α-T-Ac:
-
d3-α-T acetate
- d6-α-T-Ac:
-
d6-α-T acetate
- EDTA:
-
ethylenediaminetetraacetic acid
- GC-MS:
-
gas chromatography-mass spectrometry
- HDL:
-
high density lipoprotein
- IDL:
-
intermediate density lipoprotein
- LDL:
-
low density lipoprotein
- RBC:
-
red blood cells
- VLDL:
-
very low density lipoprotein
References
Bjornson, L.K., Kayden, H.J., Miller, E., and Moshell, A.N. (1976)J. Lipid Res. 17, 343–352.
Behrens, W.A., Thompson, J.N., and Madere, R. (1982)Am. J. Clin. Nutr. 35, 691–696.
Kayden, H.J., and Traber, M.G. (1987) inClinical and Nutritional Aspects of Vitamin E (Hayaishi, O., and Mino, M., eds.) pp. 129–138, Elsevier Science Publishers B.V. Amsterdam, Netherlands.
Traber, M.G., Olivercrona, T., and Kayden, H.J. (1985)J. Clin. Invest. 75, 1729–1734.
Traber, M.G., and Kayden, H.J. (1984)Am. J. Clin. Nutr. 40, 747–751.
Ingold, K.U., Burton, G.W., Foster, D.O., Hughes, L., Lindsay, D.A., and Webb, A. (1987)Lipids 22, 163–172.
Ingold, K.U., Hughes, L., Slaby, M., and Burton, G.W. (1987)J. Labelled Compds. Radiopharm. 24, 817–831.
Cheng, S.C., Burton, G.W., Ingold, K.U., and Foster, D.O. (1987)Lipids 22, 469–473.
Hatam, L.J., and Kayden, H.J. (1979)J. Lipid Res. 20, 639–645.
Weintraub, M.S., Eisenberg, S., and Breslow, J.L. (1987)J. Clin. Invest. 79, 1110–1119.
Traber, M.G., Kayden, H.J., and Rindler, M. (1987)J. Lipid Res. 28, 1350–63.
Kayden, H.J., and Bjornson, L.K. (1972)Ann. N.Y. Acad. Sci. 203, 127–140.
Bjornson, L.K., Gniewkowski, C., and Kayden, H.J. (1975)J. Lipid Res. 16, 39–53.
Gotto, A.M., Pownall, H.J., and Havel, R.J. (1986)Methods Enzym. 128, 3–41.
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Traber, M.G., Ingold, K.U., Burton, G.W. et al. Absorption and transport of deuterium-substituted 2R,4′R,8′R-α-tocopherol in human lipoproteins. Lipids 23, 791–797 (1988). https://doi.org/10.1007/BF02536223
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DOI: https://doi.org/10.1007/BF02536223