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Cell membrane localization of long chain C24−C30 fatty acids in two marine demosponges

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Lipids

Abstract

Subcellular fractionation by differential centrifugation was performed on two previously unstudied marine sponges (Reniera sp. andPseudaxinyssa sp.) that represent both major subclasses of the Demospongiae. Long chain fatty acids (LCFA) with 24–30 carbon units were found as major constituents of cell membrane isolates of both sponges. Most LCFA were polyunsaturated and were constituents of the phospholipids, which are typical membrane lipids, and in particular the amino-phospholipids. The LCFA composition of phospholipids from whole sponge tissue was shown to provide a reliable indication of the LCFA composition of cell membrane phospholipids in the sponges studied. An unusual triply branched C16 isoprenoid fatty acid, 4,8,12-trimethyltridecanoic acid, also was identified as a cell membrane acid in the spongePseudaxinyssa sp.

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Abbreviations

BHT:

butylated hydroxytoluene

EDTA:

ethylenediamine tetraacetic acid

FAME:

fatty acid methyl ester

LCFA:

long chain fatty acid

LCPUFA:

long chain polyunsaturated fatty acid

LCUFA:

long chain unsaturated fatty acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PS:

phosphatidylserine

PUFA:

polyunsaturated fatty acid

TMTD:

4,8,12-trimethyltridecanoic acid

UFA:

unsaturated fatty acid

A:

archaeocytes

CC:

choanocyte chambers

ECL:

equivalent chain length

EM:

electron microscopy

F:

flagella

FSW:

filtered seawater

GC:

gas chromatography

GC-MS:

gas chromatography-mass spectrometry

MV:

microvilli

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Authors and Affiliations

  1. Department of Chemistry, Stanford University, 94305, Stanford, CA

    Mishelle P. Lawson, Janice E. Thompson & Carl Djerassi

Authors
  1. Mishelle P. Lawson
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  2. Janice E. Thompson
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  3. Carl Djerassi
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Additional information

Part 16 of “Phospholipid Studies of Marine Organisms.” For Part 15 in this series see Dasgupta, A., Ayanoglu, E., Tomer, K.B., and Djerassi, C. (1987)Chem. Phys. Lipids 43, 101–111.

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Lawson, M.P., Thompson, J.E. & Djerassi, C. Cell membrane localization of long chain C24−C30 fatty acids in two marine demosponges. Lipids 23, 741–749 (1988). https://doi.org/10.1007/BF02536215

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  • DOI: https://doi.org/10.1007/BF02536215

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