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Integral lipids of human hair


It has long been recognized that hair is coated with nonpolar lipids originating in the sebaceous glands, and recently it has been shown that hair also contains cholesterol sulfate and small amounts of ceramides, similar to those found in the keratinized portion of the epidermis. In the present study, it is demonstrated that significant amounts of several additional lipids are tightly associated with hair in such a way as to be highly resistant to solvent extraction.

These integral hair lipids included cholesterol sulfate (3.3 mg/g of extracted hair), cholesterol (0.6 mg/g), fatty alcohols (0.2 mg/g) and free fatty acids (4.3 mg/g). The principal fatty acid, comprising 40% of the total fatty acids, was identified as 18-methyl-eicosanoic acid by cochromatography with authentic standard on gas-liquid chromatography (GLC) and by mass spectrometry (MS).

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equivalent chain lengths


gas-liquid chromatography


mass spectrometry


thin-layer chromatography


  1. 1.

    Chapman, R.E. (1986) inBiology of the Integument, (Bereiter-Hahn, J., Matoltsy, A.G., and Richards, K.S., eds.) pp. 293–311, Springer-Verlag, Berlin, West Germany.

    Google Scholar 

  2. 2.

    Birbeck, M.S.C., and Mercer, E.H. (1956)Nature 178, 985–986.

    PubMed  CAS  Article  Google Scholar 

  3. 3.

    Rogers, G.E. (1959)Ann. N.Y. Acad. Sci. 83, 378–399.

    PubMed  CAS  Google Scholar 

  4. 4.

    Parakkal, P.F., and Matoltsy, A.G. (1964)J. Invest. Dermatol. 43, 23–34.

    Article  Google Scholar 

  5. 5.

    Wix, M.A., Wertz, P.W., and Downing, D.T. (1987)Comp. Biochem. Physiol. 86B, 671–673.

    CAS  Google Scholar 

  6. 6.

    Nicolaides, N., Fu, H.C., and Rice, G.R. (1968)J. Invest. Dermatol. 51, 83–89.

    PubMed  CAS  Article  Google Scholar 

  7. 7.

    Stewart, M.E., Downing, D.T., and Strauss, J.S. (1983)Dermatol. Clin. 1, 335–344.

    Google Scholar 

  8. 8.

    Wertz, P.W., and Downing, D.T. (1987)Biochim. Biophys. Acta 917, 108–111.

    PubMed  CAS  Google Scholar 

  9. 9.

    Swartzendruber, D.S., Wertz, P.W., Madison, K.C., and Downing, D.T. (1987)J. Invest. Dermatol. 88, 709–713.

    PubMed  CAS  Article  Google Scholar 

  10. 10.

    Downing, D.T. (1968)J. Chromatogr. 38, 91–99.

    PubMed  CAS  Article  Google Scholar 

  11. 11.

    Downing, D.T., Kranz, F.H., and Murray, K.E. (1960)Aust. J. Chem. 13, 80–94.

    CAS  Article  Google Scholar 

  12. 12.

    Nicolaides, N., and Apon, J.M.B. (1976)Lipids 11, 781–790.

    PubMed  CAS  Article  Google Scholar 

  13. 13.

    Abrahamsson, S., Stallberg-Stenhagen, S., and Stenhagen, E. (1963)Prog. Chem. Fats Other Lipids 7, 1–164.

    Google Scholar 

  14. 14.

    Wertz, P.W., and Downing, D.T. (1986)Biochem. Biophys. Res. Commun. 137, 992–997.

    PubMed  CAS  Article  Google Scholar 

  15. 15.

    Nicolaides, N., Fu, H.C., Ansari, M.N.A., and Rice, G.R. (1972)Lipids 7, 506–517.

    PubMed  CAS  Article  Google Scholar 

  16. 16.

    Zuneda, M.C., Guillenea, J.J., Dominguez, J.B., Prado, A., and Goni, F.M. (1984)Lipids 19, 223–228.

    PubMed  CAS  Article  Google Scholar 

  17. 17.

    Song, Y., Sawa, T., Tsuvhiya, M., Kondo, S., Hamada, M., and Umezawa, H. (1981)J. Antibiot. Tokyo 34, 980–983.

    PubMed  CAS  Google Scholar 

  18. 18.

    Kaneda, T., and Smith, E.J. (1980)Can. J. Microbiol. 26, 893–898.

    PubMed  CAS  Article  Google Scholar 

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Correspondence to Philip W. Wertz.

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Wertz, P.W., Downing, D.T. Integral lipids of human hair. Lipids 23, 878–881 (1988).

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  • Fatty Acid Methyl Ester
  • Stratum Corneum
  • Fatty Alcohol
  • Free Sterol
  • Stearyl Alcohol