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In vivo determination of triglyceride secretion using radioactive glycerol in rats fed different dietary saturated fats

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Lipids

Abstract

Our objective was to determine the relative rates ofin vivo triglyceride (TG) secretion and the composition of very low density lipoproteins (VLDL) in rats fed different dietary saturated fats. Male Sprague-Dawley rats (150–200 g) were fed diets containing 16% corn oil, or 14% butterfat, 14% beef tallow, 14% olive oil, or 14% coconut oil plus 2% corn oil for 5 wk. Changes in plasma TG specific radioactivity were determined in individual, unanesthetized fasted rats after injection of 100 μCi [2-3H]glycerol. Nonlinear regression analysis using a 2-compartment model was used to determine the fractional rate constant for TG turnover in plasma. The plasma TG pool was 33–40% larger with beef tallow than with corn, olive or coconut oil feeding (p<0.05), and 20% larger with beef tallow than with butterfat feeding. The rate of TG secretion into plasma (mg/min/100 g body weight) was 60% higher in animals fed beef tallow than corn or coconut oil (p<0.05) and 26–33% higher in animals fed beef tallow than olive oil or butterfat. Differences in VLDL composition (% wt) were also noted. Our data suggest that greater TG secretion is the primary factor contributing to the larger TG pool with ingestion of beef tallow relative to butterfat, corn or coconut oil. These results suggest that different dietary saturated fats have unique effects on TG metabolism in rats.

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Abbreviations

ANOVA:

analysis of variance

apo:

apolipoprotein

PL:

phospholipid

TG:

triglyceride

VLDL:

very low density lipoprotein

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Author notes

  1. John B. Lasekan

    Present address: Kraft General Foods, Technical Research Center, T22-1, 250 North Street, 10625, White Plains, NY

Authors and Affiliations

  1. Department of Nutritional Sciences, 1415 Linden Drive, 53706, Madison, WI

    Hui-Chuan Lai, John B. Lasekan, Huan Yang & Denise M. Ney

  2. Departments of Statistics and Plant Pathology, University of Wisconsin-Madison, 53706, Madison, WI

    Murray K. Clayton

Authors
  1. Hui-Chuan Lai
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  2. John B. Lasekan
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  3. Huan Yang
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  4. Murray K. Clayton
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  5. Denise M. Ney
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Lai, HC., Lasekan, J.B., Yang, H. et al. In vivo determination of triglyceride secretion using radioactive glycerol in rats fed different dietary saturated fats. Lipids 26, 824–830 (1991). https://doi.org/10.1007/BF02536164

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  • DOI: https://doi.org/10.1007/BF02536164

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