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Incorporation of n−3 fatty acids into WB-F344 cell phospholipids inhibits gap junctional intercellular communication

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Lipids

Abstract

In this investigation, we demonstrate that rat liver epithelial (WB-F344) cells grown in medium supplemented with n−3 fatty acids (FA) results in the inhibition of gap junctional intercellular communication (GJIC). Cells incubated for 48 hr in medium containing 50 μM α-linolenate (18∶3n−3) resulted in a 60% inhibition of GJIC, compared to control cells, while treatment with γ-linolenate (18∶3n−6) had no effect. Supplementation with octadecatetraenoate (18∶4n−3), eicosapentaenoate (20∶5n−3), and docosahexaenoate (22∶6n−3), inhibited GJIC by 42%, 28%, and 18%, respectively. Incubation with each of the n−3 FA markedly increased the total n−3 FA content of cellular phospholipids (PL). Growing cells in medium containing 50 μM arachidonate (20∶4n−6) plus 50 μM 18∶3n−3 partially attenuated the inhibition of GJIC induced by 18∶3n−3. The mechanism by which n−3 FA inhibit GJIC remains to be determined.

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Abbreviations

ACAS:

anchored cell analysis and sorting

AA:

20∶4n−6, arachidonate

ALN:

18∶3n−3, α-linolenate

DHA:

22∶6n−3 docosahexaenoate

EPA:

20∶5n−3, eicosapentaenoate

FA:

fatty acid(s)

FRAP:

fluorescence redistribution after photobleaching

GJIC:

gap junctional intercellular communication

GLN:

18∶3n−6, γ-linolenate

LDH:

lactate dehydrogenase

ODA:

18∶4n−3, octadecatetraenoate

PL:

phospholipid(s)

SEM:

standard error of the mean

TPA:

12-O-tetradecanoyl-phorbol 13-acetate

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Author notes

  1. Clare M. Hasler

    Present address: Molecular Mechanisms of Tumor Promotion Section, Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, 20892, Bethesda, MD

Authors and Affiliations

  1. Department of Food Science and Human Nutrition, Michigan State University, 48824, East Lansing, Michigan

    Clare M. Hasler, James E. Trosko & Maurice R. Bennink

Authors
  1. Clare M. Hasler
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  2. James E. Trosko
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  3. Maurice R. Bennink
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Hasler, C.M., Trosko, J.E. & Bennink, M.R. Incorporation of n−3 fatty acids into WB-F344 cell phospholipids inhibits gap junctional intercellular communication. Lipids 26, 788–792 (1991). https://doi.org/10.1007/BF02536159

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  • DOI: https://doi.org/10.1007/BF02536159

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