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Improved resolution of31P nuclear magnetic resonance spectra of phospholipids from brain

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Lipids

Abstract

A method is described wherein the resolution of31P nuclear magnetic resonance spectra of the lipophilic fraction from a Bligh-Dyer extract of rat brain can be enhanced. The lipids are dispersed as micelles in aqueous solution with sodium deoxycholate, and spectral resolution is further optimized by adjusting the pH and by removing ions from the solution. The application of the method to the study of aging in rat brain serves as an example.

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Abbreviations

CerCho:

sphingomyelin

EDTA:

ethylenediaminetetraacetic acid

NMR:

nuclear magnetic resonance

PA:

phosphatidic acid

PtdCho:

phosphatidylcholine

PtdEtn:

phosphatidylethanolamine

PtdIns:

phosphatidylinositol

PtdSer:

phosphatidylserine

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Author information

Authors and Affiliations

  1. Institute for Research on Senescence, Sigma Tau, 00040, Pomezia (Rome), Italy

    G. Capuani, T. Aureli & M. T. Ramacci

  2. Department of Chemistry, University of Rome “La Sapienza”, Rome, Italy

    A. Miccheli, M. E. Di Cocco & M. Delfini

Authors
  1. G. Capuani
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  2. T. Aureli
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  3. A. Miccheli
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  4. M. E. Di Cocco
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  5. M. T. Ramacci
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  6. M. Delfini
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Capuani, G., Aureli, T., Miccheli, A. et al. Improved resolution of31P nuclear magnetic resonance spectra of phospholipids from brain. Lipids 27, 389–391 (1992). https://doi.org/10.1007/BF02536154

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  • DOI: https://doi.org/10.1007/BF02536154

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