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Lipids

, Volume 28, Issue 6, pp 539–547 | Cite as

Interrelationship of stearic acid content and triacylglycerol composition of lard, beef tallow and cocoa butter in rats

  • Cathy C. Monsma
  • Denise M. Ney
Article

Abstract

We investigated modes whereby stearic acid (18∶0) exerts a neutral or cholesterol-lowering effect using dietary fats which provided graded levels of 18∶0 and distinct triacylglycerol (TAG) profiles. Male Sprague-Dawley rats (150–175 g) were fed diets containing 0.2% cholesterol and 16% fat from corn oil, or from 1% corn oil plus 15% lard (13.2% 18∶0), beef tallow (19.2% 18∶0) or cocoa butter (34.7% 18∶0) for 3 wk, and then killed in a fasted or fed state. Chylomicron (CM) fatty acid profiles suggested reduced absorption of 18∶0 with greater 18∶0 intake. CM TAG profiles indicated a reduction or loss of two TAG species compared to the TAG profiles of the stearate-rich diets: 1-palmitoyl-2-oleoyl-3-stearoyl glycerol (POS) and 1,3-distearoyl-2-oleoyl glycerol (SOS). Hepatic total cholesterol concentrations were 54–77% lower (P<0.01) in the cocoa butter-fed than the lard- and beef tallow-fed groups. The cocoa butter group showed a significantly lower ratio of high-density lipoprotein esterified/free cholesterol than all other groups. Hepatic stearoyl-CoA and oleoyl-CoA concentrations, the substrate and product for hepatic δ9 desaturase, were not significantly different for corn oil-fed and cocoa butter-fed groups in spite of a large difference in 18∶0 intake. These data suggest that the neutral or cholesterol-lowering effect of 18∶0 is not due to hepatic conversion of stearic to oleic acid, and that POS and SOS are poorly absorbed from stearate-rich dietary fats.

Keywords

Cholesteryl Ester Cocoa Butter Beef Tallow Stearic Acid Content Esterify Cholesterol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

ANOVA

analysis of variance

Cn

total acyl carbon number

CM

chylomicron

E/F

esterified/free

EC

esterified cholesterol

FAME

fatty acid methyl esters

GC-FID

gas chromatography-flame-ionization detection

GLM

general linear models

HDL

high-density lipoprotein

LDL

low-density lipoprotein

L

linoleic acid

O

oleic acid

P

palmitic acid

POP

1,3-dipalmitoyl-2-oleoyl glycerol

POS

1-palmitoyl-2-oleoyl-3-stearoyl glycerol

SOS

1,3-distearoyl-2-oleoyl glycerol

TAG

triacylglycerol

TG

triglycerides

VLDL

very low density lipoprotein

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Copyright information

© American Oil Chemists’ Society 1993

Authors and Affiliations

  • Cathy C. Monsma
    • 1
  • Denise M. Ney
    • 1
  1. 1.Department of Nutritional SciencesUniversity of WisconsinMadison

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