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A multivariate optimization of triacylglycerol analysis by high-performance liquid chromatography

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Lipids

A Corrections to this article was published on 01 October 1993

Abstract

In the present study we have used statistical experimental design and multivariate optimization to formally optimize a reversed-phase high-performance liquid chromatography method for the analysis of triacylglycerol molecular species of natural oils. The optimal conditions found were, on an octadecylsilan-column, from acetonitrile/isooctane (90∶10, vol/vol) to acetonitrile/ethanol/isooctane (40∶35∶25, by vol), at a column temperature of 50°C and a flowrate of 1.5mL/min using a negative exponential gradient profile. Several examples of separations of natural seed and animal oils,i.e., soybean oil, rapeseed oil, palm oil, linseed oil, tallow and fish oil, are given. A version of the equivalent carbon number concept, utilizing the Snyder polarity index, was used to identity the molecular species.

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Abbreviations

Ad:

arachidic acid

CN:

carbon number

CRS:

chromatographic resolution statistic

D:

decanoic acid

ECN:

equivalent carbon number

Hd:

heptadecanoic acid

HPLC:

high-performance liquid chromatography

HRGC:

high resolution gas chromatography

L:

linoleic acid

La:

lauric acid

Ln:

linolenic acid

M:

myristic acid

Nd:

nonadecanoic acid

O:

oleic acid

P:

palmitic acid

P′:

Snyders polarity index

Pd:

pentadecanoic acid

PN:

partition number

PLS:

partial least squares correlations in latent variables

P:

palmitic acid

RP-HPLC:

reversed-phase high-performance liquid chromatography

St:

stearic acid

TAG:

triacylglycerol

UV:

ultraviolet

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Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, Stockholm University, Stockholm, Sweden

    Magnus H. J. Bergqvist

  2. Karlshamns Lipid-Teknik AB, P.O. Box 66 86, S-113 84, Stockholm, Sweden

    Peter Kaufmann

Authors
  1. Magnus H. J. Bergqvist
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  2. Peter Kaufmann
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Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/BF02537513.

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Bergqvist, M.H.J., Kaufmann, P. A multivariate optimization of triacylglycerol analysis by high-performance liquid chromatography. Lipids 28, 667–675 (1993). https://doi.org/10.1007/BF02536064

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