Abstract
The present study examined the effect of varying dietary linoleate intake (0.01, 0.24, 2.4, 24, 80 or 160 g/kg diet) for 24 weeks on the distribution of triacylglycerol (TG) molecular species in rat epididymal adipose tissue. Adipose TG fractions were purified by thin-layer chromatography and separated into different molecular species by reversephase high-performance liquid chromatography. The identification of TG species was based on fatty acid composition, retention time and the theoretical carbon number. When the dietary 18∶2n−6 content was equal to or less than 24 g/kg, no significant amounts of n−6 fatty acids (mainly 18∶2n−6) were observed in adipose tissue TG despite the fact that the levels of 20∶4n−6 in liver phospholipids increased significantly. There were 12 major molecular species in adipose tissue when the dietary 18∶2n−6 content was less than 2.4 g/kg. When the diteary 18∶2n−6 content reached 24 g/kg, an additional six TG species containing one, two or three molecules of 18∶2n−6 were observed. The levels of TG molecules containign two or three 18∶2n−6 residues were further increased when the diet contained very large amounts of linoleic acid (160 g/kg). Conversely, those TG species containing only one 18∶2n−6 residue became less abundant. It is suggested that the accumulation of these linoleate-rich TG molecular species in adipose tissue, particularly di- and trilinoleoyl containing TG, is the result of an adequate or an excessive intake of linoleic acid.
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Abbreviations
- GLC:
-
gas-liquid chromatography
- HPLC:
-
high-performance liquid chromatography
- L:
-
linoleic acid (18∶2n−6)
- O:
-
oleic acid (18∶1n−9)
- P:
-
palmitic acid (16∶0)
- PL:
-
phospholipids
- Po:
-
palmitoleic acid (16∶1n−7)
- S:
-
stearic acid (18∶0)
- TG:
-
triacylglycerols
- TLC:
-
thin-layer chromatography
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Huang, YS., Lin, X., Smith, R.S. et al. Effect of dietary linoleic acid content on the distribution of triacylglycerol molecular species in rat adipose tissue. Lipids 27, 711–715 (1992). https://doi.org/10.1007/BF02536030
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DOI: https://doi.org/10.1007/BF02536030