Skip to main content
SpringerLink
Log in

Specificity of soybean lipoxygenase-1 in hydrated reverse micelles of sodiumbis(2-ethylhexyl)sulfosuccinate (aerosol OT)

  • Article
  • Published:
Lipids

Abstract

Soybean lipoxygenase-1 (EC 1.13.11.33) was purified by fast protein liquid chromatography on a MONO Q column and studied with respect to the conversion of linoleic and arachidonic acids in reverse micelles of sodiumbis(2-ethylhexyl)sulfosuccinate inn-octane. In this system the specific activities were lower by one order of magnitude than those in the corresponding aqueous system. High-performance liquid chromatography analyses indicated the predominant formation of 13S-hydroperoxy-9Z,11E-octadecadienoic acid (13-HpODE) from linoleic acid and of 15S-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (15) from arachidonic acid both in the aqueous system and in the reverse micelles. After sedimentation of the hydrated reverse micelles by ultracentrifugation, both linoleic acid and 13-HpODE were found to be enriched in the micelles with only small amounts of these compounds present inn-octane. It is proposed that substrates and products of the lipoxygenase reaction are located mainly in the surfactant shell of the hydrated reverse micelles and reach the micelle-entrapped enzyme by diffusion into the aqueous interior space.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AOT:

Aerosol OT, dioctylsulfosuccinate sodium salt, sodiumbis(2-ethylexyl)sulfosuccinate

13-HpODE:

13S-hydroperoxy-9Z,11E-octadecadienoic acid

15-HpETE:

15S-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid

DNP:

2,4-dinitrophenol

MOPS:

4-morpholino-propanesulfonic acid

HPLC:

high-performance liquid chromatography

TLC:

thin-layer chromatography

UV:

ultraviolet

References

  1. Martinek, K., Levashov, A.V., Klyachko, N., Khmelnitsky, Y.L., and Berezin, I.V. (1986)Eur. J. Biochem. 155, 453–468.

    Article  PubMed  CAS  Google Scholar 

  2. Luisi, P.L., Giomini, M., Pileni, M.P., and Robinson, B.H. (1988)Biochim. Biophys. Acta 947, 209–246.

    PubMed  CAS  Google Scholar 

  3. Luisi, P.L., Luthi, P., Tomka, I., Prenosyl, J., and Pande, A. (1984)Ann. N.Y. Acad. Sci. 434, 549–557.

    CAS  Google Scholar 

  4. Kurganov, B.I., Shkarina, T.N., Malakhova, E.A., Davydov, D.R., and Chebotareva, N.A. (1989)Biochimie 71, 573–578.

    Article  PubMed  CAS  Google Scholar 

  5. Butovich, I.A., Mogilevich, T.V., and Kukhar, V.P. (1989)Ukrain. Biochem. J. 61, 54–59.

    CAS  Google Scholar 

  6. Hochkoeppler, A., and Palmieri, S. (1990)Biotechnol. Bioenerg. 36, 672–677.

    Article  CAS  Google Scholar 

  7. Kühn, H., Schewe, T., and Rapoport, S.M. (1986)Adv. Enzymol. Rel. Areas Molec. Biol., Vol. 58, pp. 273–311, John Wiley & Sons, New York.

    Google Scholar 

  8. Bild, G.S., Ramadoss, C.S., Lim, S., and Axelrod, B. (1977)Biochem. Biophys. Res. Commun. 74, 949–954.

    Article  PubMed  CAS  Google Scholar 

  9. Axelrod, B., Cheesbrough, T.M., and Laakso, S. (1981)Methods Enzymol. 71, 441–451.

    Article  CAS  Google Scholar 

  10. Schewe, T., Kühn, H., and Rapoport, S.M. (1987) inProstaglandins and Related Substances—A Practical Approach (Benedetto, C., McDonald-Gibson, R.G., Nigam, S., and Slater, T.F., eds.) pp. 229–242, IRL Press, Oxford.

    Google Scholar 

  11. Kühn, H., Wiesner, R., Lankin, V.Z., Nekrasov, V., Alder, L., and Schewe, T. (1987)Anal. Biochem. 160, 24–34.

    Article  PubMed  Google Scholar 

  12. Khmelnitzky, Y.L., Levashov, A.V., and Martinek, K. (1981)Vestnik of Moscow University, Khimija Ser. 2, Vol. 22, 195–199.

    Google Scholar 

  13. Ishikawa, H., Noda, K., and Oka, T. (1990)J. Ferment. Bioenerg. 70, 381–385.

    Article  CAS  Google Scholar 

  14. Ruckenstein, E., and Karpe, P. (1990)Biotechnol. Letters 12, 817–820.

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Tatyana N. Shkarina

    Present address: All-Union Vitamin Research Institute, Nauchny proezd 14a, 117820, Moscow, Russia

Authors and Affiliations

  1. Institute of Biochemistry of the Medical School (Charité), Humboldt University, Hessische Str. 3-4, D-O-1040, Berlin, Germany

    Tatyana N. Shkarina, Hartmut Kühn & Tankred Schewe

Authors
  1. Tatyana N. Shkarina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hartmut Kühn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tankred Schewe
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Shkarina, T.N., Kühn, H. & Schewe, T. Specificity of soybean lipoxygenase-1 in hydrated reverse micelles of sodiumbis(2-ethylhexyl)sulfosuccinate (aerosol OT). Lipids 27, 690–693 (1992). https://doi.org/10.1007/BF02536026

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02536026

Keywords

Search

Navigation