Abstract
The present study was designed to investigate the effect of ethanol (EtOH) dose on low density lipoprotein (LDL) and platelet composition. Male squirrel monkeys were divided into three groups designated Control, Low, and High EtOH, and fed isocaloric liquid diets containing 0%, 12%, and 24% of calories as EtOH, respectively. After four months of treatment, monkeys fed the 12% alcohol dose had LDL and platelet cholesterol concentrations similar to Controls. By contrast, platelet membranes from High EtOH animals contained significantly more cholesterol which was associated with higher levels of plasma LDL cholesterol and apolipoprotein B. Blood platelet count, size, and mass were similar for all groups and circulating platelet aggregates were absent in the two alcohol cohorts. Despite elevations in platelet cholesterol mass and thromboxane A2 (TXA2) precursor, phospholipid arachidonate, platelet responsiveness, measured as thromboxane formed in response to a collagen challengein vitro, and the cholesterol/phospholipid molar ration, were not significantly altered by high dose alcohol. Normal platelet activity in High EtOH monkeys may have resulted from a significant increase in the platelet phospholipid polyunsaturated/saturated fatty acid ratio and a non-significant increase in platelet phospholipid mass, both of which would have a fluidizing effect on platelet membranes. Our data indicate that low EtOH intake has no effect on platelet composition and function while unfavorable platelet cholesterol enrichment following consumption of high dose ethanol may arise from elevations in plasma LDL. Potentially thrombogenic (TXA2 formation, platelet aggregate formation) consequences of increased platelet cholesterol in squirrel monkeys fed high levels of alcohol may be averted by compensatory physicochemical changes in platelet membrane lipid composition although persistent elevations in circulating LDL may contribute to increased risk of cardiovascular disease.
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Stampfer, M.J., Colditz, G.A., Willett, W.C., Speizer, F.E., and Hennekens, C.H. (1988)N. Engl. J. Med. 319, 267–273.
Moore, R.D., and Pearson, T.A. (1986)Medicine 65, 242–267.
Criqui, M.H. (1986)Alcoholism: Clin. Exp. Res. 10, 564–572.
Karanian, J.W., Shingu, T., D'Souza, N.B., Engler, M.M., Engler, M.B., and Salem, Jr. N. (1986) inCardiovascular Disease (Gallo, L. ed.), p. 70, Plenum Press, New York.
Klatsky, A.L., Armstrong, M.A., and Friedman, G.D. (1989)Stroke 20, 741–746.
Diamond, I. (1989)N. Engl. J. Med. 320, 458–459.
Hojnacki, J.L., Brown, J.E., Mulligan, J.J., Hagan, S.M., Mahony, K.E., Witzgall, S.K., Osmolski, T.V., and Barboriak, J.J. (1987)Circulation 76, 36A.
Cluette-Brown, J., Mulligan, J., Osmolski, T., Deschenes, R., and Hojnacki, J. (1987)Clin. Res. 35, 785A.
Witzgall, S.K., Mahony, K., Deschenes, R.N., Mulligan, J.J., Cluette-Brown, J., Osmolski, T.V., Barboriak, J.J., and Hojnacki, J.L. (1988)Atherosclerosis 71, 235–241.
Hojnacki, J., Cluette-Brown, J., Mulligan, J., Hagan, S., Mahony, K., Witzgall, S., Osmolski, T., and Barboriak, J. (1988)Alcoholism: Clin. Exp. Res. 12, 149–154.
Mitchell, M.C., and Herlong, H.F. (1986)Ann. Rev. Nutr. 6, 457–474.
Lieber, C.S. (1976)Sci. Amer. 234, 25–33.
Karanian, J.W., Stojanov, M., and Salem, Jr. N. (1985)Prostaglandins Leukotrienes Med. 20, 175–186.
Mikhailidis, D.P., Jenkins, W.J., and Jeremy, J.Y. (1984)N. Engl. J. Med. 311, 537–538.
Landolfi, R., and Steiner, M. (1984)Blood 64, 679–682.
Mehta, P., Mehta, J., Lawson, D., and Patel, S. (1987)Thromb. Res. 48, 653–661.
Mikhailidis, D.P., Jeremy, J.Y., Baradas, M.A., Green, N., and Dandona, P. (1983)Brit. Med. J. 287, 1495–1498.
Guivernau, M., Baraona, E., and Lieber, C.S. (1987)J. Pharmacol. Exp. Therap. 240, 59–64.
Hwang, D.H., LeBlanc, P., and Chanmugan, P. (1981)Lipids 16, 583–588.
Oates, J.A., Fitzgerald, G.A., Branch, R.A., Jackson, E.K., Knapp, H.R., and Roberts, L.J. (1988)N. Engl. J. Med. 319, 689–767.
Toivanen, J., Ylikorkala, O., and Viinikka, L. (1983)Thrombosis Res. 33, 1–8.
Beitz, J., Schollberg, K., Fischer, S., Jaross, W., Hanefeld, M., and Mest, H.J. (1988)Biomed. Biochim. Acta 47, 181–187.
Triau, J.E., Meydani, S.N., and Schaefer, E.J. (1988)Arteriosclerosis 8, 810–818.
Holland, J.A., Pritchard, K.A., Rogers, N.J., and Stemerman, M.B. (1988)Amer. J. Pathol. 132, 474–478.
Larrue, J. (1988)Pharmacol. Res. Commun. 20, Suppl III, 109–119.
Beitz, J., Kuklinski, B., Taube, C., and Mest, H.J. (1989)Prostaglandins Leukotrienes Med. 35, 37–39.
Pomerantz, K.B., Tall, A.R., Feinmark, S.J., and Cannon, P.J. (1984)Circ. Res. 54, 554–565.
Fleisher, L.N., Tall, A.R., Witte, L.D., Miller, R.W., and Cannon, P.J. (1986)J. Biol. Chem. 257, 6653–6655.
Pomerantz, K.B., and Hajjar, D.P. (1989)Arteriosclerosis 9, 413–429.
Aviram, M., and Brook, J.G. (1983)Atherosclerosis 46, 259–268.
Guivernau, M., Baraona, E., Soong, J., and Lieber, C.S. (1989)Biochem. Pharmacol. 38, 503–508.
Nordoy, A., Svensson, B., Weibe, D., and Hoak, J. (1987)Circ. Res. 43, 527–533.
Beitz, J., Panse, M., Fischer, S., Hora, C., and Forster, W. (1983)Prostaglandins 26, 885–892.
Beitz, J., and Forster, W. (1980)Biochim. Biophys. Acta 620, 352–355.
Beitz, J., and Mest, H.J. (1986)Prostaglandins Leukotrienes Med. 23, 303–309.
Hwang, D.H., Chanmugam, P., Hymel, G., and Boudreau, M. (1987)Prostaglandins Leukotrienes Med. 26, 299–305.
Pediconi, M., Colombo, C., and Galli, C. (1985)Prostaglandins 30, 313–322.
Wybenga, D.R., Pileggi, U.J., Dirstine, P.H., and DiGiorgia, J. (1970)Clin. Chem. 16, 980–984.
Warnick, G.R., and Albers, J.J. (1978)J. Lipid Res. 19, 65–76.
Cluette-Brown, J.E., Mulligan, J.J., Doyle, K., Hagan, S.M., Osmolski, T., and Hojnacki, J.L. (1986)Proc. Soc. Exp. Biol. Med. 182, 409–413.
Hojnacki, J.L., Mulligan, J.J., Couette-Brown, J.E., Igoe, F.D., and Osmolski, T. (1986)Proc. Soc. Exp. Biol. Med. 182, 414–418.
Cluette-Brown, J., Mulligan, J., Igoe, F., Doyle, K., and Hojnacki, J. (1985)Proc. Soc. Exp. Biol. Med. 178, 495–499.
Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.
Hojnacki, J.L., and Smith, S.C. (1974)J. Chromatogr. 90, 365–367.
Cohen, P., and Derksen, A. (1969)Brit. J. Haematol. 17, 359–371.
Mulligan, J.J., Cluette-Brown, J.E., Noring, R., Igoe, F.D., Chong, J., and Hojnacki, J.L. (1985)Res. Commun. Chem. Pathol. Pharmacol. 47, 181–202.
Bartlett, G.R. (1959)J. Biol. Chem. 234, 466–468.
Kramer, R.M., Jakubowski, J.A., Vaillancourt, R., and Deykin, D. (1982)J. Biol. Chem. 257, 6844–6849.
Jakubowski, J.A., Stampfer, M.J., Vaillancourt, R., and Deykin, D. (1985)Brit. J. Haematol. 60, 635–642.
Wu, K.K., and Hoak, J.C. (1974)Lancet 2, 924–926.
Lum, G., and Gambino, S.R. (1972)Clin. Chem. 18, 358.
Rudel, L.L., Parks, J.S., Johnson, F.L., and Babiak, J. (1986)J. Lipid Res. 27, 465–474.
Miller, N.E., and Miller, G.J. (1984)Clinical and Metabolic Aspects of High Density Lipoproteins, Vol. 3, pp. 266–267. Elsevier, New York.
Katan, M.B. (1984) inClinical and Metabolic Aspects of High-Density Lipoproteins (Miller, N.E., and Miller, G.J., eds.) Vol. 3, p. 103, Elsevier, New York.
Kannel, W.B. (1988)Proc. Nutr. Soc. 47, 99–110.
Hegsted, M.D., and Ausman, L.M. (1988)J. Nutr. 118, 1184–1189.
Baraona, E., and Lieber, C.S. (1979)J. Lipid Res. 20, 289–295.
Lieber, C.S., and Savolainen, M. (1984)Alcoholism: Clin. Exp. Res. 8, 409–423.
Taskinen, M., Valimaki, M., Nikkila, E.A., Kuusi, T., and Ylikahri, R. (1985)Metabolism 34, 112–119.
Rudel, L.L., Leathers, C.W., Bond, M.G., and Bullock, B.C. (1981)Arteriosclerosis 1, 144–155.
Shepherd, R.J., Cox, M., and West, C. (1980)Atherosclerosis 35, 287–300.
Mikhailidis, D.P., Jeremy, J.Y., Barradas, M.A., and Dandona, P. (1985)Blood 65, 777.
Kontula, K., Viinikka, L., Ylikorkala, O., and Ylikahri, R. (1982)Life Sci. 31, 261–264.
Pennington, S.N., Woody, D.G., and Rumbley, R.A. (1982)Prostaglandins Leukotrienes Med. 9, 151–157.
Pediconi, M., Colombo, C., and Galli, C. (1986)J. Neurochem. 46, 1458–1463.
Ouwendijk, R.J.T, Zijlstra, F.J., Wilson, J.H.P., Bonta, I.L., and Vincent, J.E. (1983)Prostaglandins Leukotrienes Med. 10, 115–122.
Beitz, A., Nikitina, N.A., Giessler, C., Beitz, J., Masaev, V.P., Perova, N.A., and Mest, H.J. (1990)Prostaglandins Leukotrienes Essential Fatty Acids 40, 57–61.
Fetkovska, N., Amstein, R., Ferracin, F., Buhler, F.R., and Pletscher, A. (1988)J. Hypertension 6, S646-S648.
Kamido, H., Matsuzawa, Y., and Tarui, S. (1988)Lipids 23, 917–923.
Hassall, D.G., Owen, J.S., and Bruckdorfer, K.R. (1983)Biochem. J. 216, 43–49.
Block, L.H., Knorr, M., Vogt, E., Locher, R., Vetter, W., Groscurth, P., Qiao, B., Pometta, D., James, R., Regenass, M., and Pletscher, A. (1988)Proc. Natl. Acad. Sci. USA 85, 885–889.
Aviram, M., Brook, J.G., Lees, A.M., and Lees, R.S. (1981)Biochem. Biophys. Res. Commun. 99, 308–318.
Aviram, M., Lees, A.M., and Lees, R.S. (1980)Clin. Res. 28, 384A.
Cooper, R.A., and Shattil, S.J. (1980)N. Engl. J. Med. 302, 49–50.
Aviram, M., and Brook, G.J. (1982)Thrombosis Res. 26, 101–109.
Aviram, M., and Deckelbaum, R.J. (1986)Metabolism 38, 343–347.
Cooper, R.A., Diloy-Puray, M., Lando, P., and Greenberg, M.S. (1972)J. Clin. Invest. 51, 3182–3192.
Schick, B.P., and Schick, P.K. (1985)Biochim. Biophys. Acta 833, 291–302.
Jakubowski, J.A., Vaillancourt, R., and Deykin, D. (1988)Arteriosclerosis 8, 436–441.
Stuart, M.J., Gerrard, J.M., and White, J.G. (1980)N. Engl. J. Med. 30, 6–10.
Fenn, C.G., and Littleton, J.M. (1982)Thromb. Haemostas. 48, 49–53.
Mulligan, J.J., Cluette-Brown, J.E., Igoe, F.D., Noring, R., Osmolski, T.V., and Hojnacki, J.L. (1986)Res. Commun. Chem. Pathol. Pharmacol. 51, 269–272.
Rand, M.L., Packham, M.A., Kinlough-Rathbon, R.L., and Mustard, J.F. (1988)Thromb. Haemostas. 59, 383–387.
Chin, J.H., and Goldstein, D.B. (1977)Mol. Pharmacol. 13, 435–441.
Rubin, E., and Rottenberg, H. (1982)Federation Proc. 41, 2465–2471.
Lin, T.N., Sun, A.Y., and Sun, G.Y. (1988)Alcoholism: Clin. Exp. Res. 12, 795–800.
Cucuianu, M.P. (1983) inBiomembranes and Cell Function (Kummerow, F.A., Benga, G., and Homes, R.P., eds.) Vol. 414, pp. 57–65, Annals NY Academy of Science, New York.
Neiman, J., Curstedt, T., and Cronholm, T. (1987)Thrombosis Res. 46, 295–301.
Anggard, E. (1983)Pharmacol. Biochem. Behav. 18, 401–407.
Fiedller, P., Wolkin, A., and Rotrosen, J. (1986)Soc. Biol. Psychiat. 21, 1344–1347.
Nelson, G.J., Kelley, D.S., Schmidt, P.C., and Serrato, C.M. (1987)Circulation, Suppl. IV, 76, 36.
Ylikorkala, O., Halmesmaki, E., and Viinikka, L. (1987)Life Sci. 41, 371–376.
Fenn, C.G., and Littleton, J.M. (1984)Brit. J. Pharmacol. 81, 175.
Rubin, R. (1989)Arch. Biochem. Biophys. 270, 99–113.
Peers, S.H., and Hoult, J.R.S. (1986)Eur. J. Pharmacol. 120, 145–150.
Cowan, D.H. (1980)Sem. Hematol. 17, 137–147.
Thompson, C.B., Jakubowski, J.A., Quinn, P.G., Deykin, D., and Valeri, C.R. (1983)J. Lab. Clin. Med. 101, 205–213.
Jakubowski, J.A., Thompson, C.B., Vaillancourt, R., Valeri, C.R. and Deykin, D. (1983)Brit. J. Haematol. 53, 503–511.
Jakubowski, J.A., and Ardlie, N.G. (1978)Atherosclerosis 31, 335–344.
Fitzgerald, G.A., Smith, B., Pederson, A.K., and Brash, A.R. (1984)N. Engl. J. Med. 310, 1065–1068.
Hillbom, M., Muuronen, A., Lowbeer, C., Anggard, E., Beving, H., and Kangassaho, M. (1985)Thromb. Haemostas. 53, 419–422.
Spector, A.A. (1988)Sem. Thromb. Hemostas. 14, 196–201.
Beitz, J., Block, H.U., Beitz, A., Muller, G., Winkler, L., Dargel, R., and Mest, H.J. (1986)Atherosclerosis 60, 95–99.
Giessler, C., Beitz, J., Mentz, P., and Forster, W. (1986)Prostaglandins Leukotrienes Med. 22, 221–234.
Desai, K., Bagget, C., Bellamy, M.F., Mistry, P., Burroughs, A.K., and Owen, J.S. (1989)Lancet 1, 693–695.
Pritchard, K.A., Wong, P.Y.-K., and Stemmerman, M.B. (1990)Amer. J. Pathol. 136, 1383–1391.
Sachinidis, A., Locher, R., Mengden, T., Steiner, A., and Vetter, W. (1989)Biochem. Biophys. Res. Commun. 163, 315–320.
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Hojnacki, J.L., Cluette-Brown, J.E., Deschenes, R.N. et al. Effect of ethanol on low density lipoprotein and platelet composition. Lipids 26, 884–890 (1991). https://doi.org/10.1007/BF02535972
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DOI: https://doi.org/10.1007/BF02535972