Abstract
Epidemiological studies suggest the existence of a strong relationship between the incidence of prostatic cancer and the intake of dietary lipids in humans. However, very little information is available on intracellular fatty acid metabolism in human prostatic tissue. The objective of this study was to identify and subsequently characterize a fatty acid binding protein of human prostatic tissue. A fatty acid binding protein (FABP) was purified and characterized from human prostatic tissue. The purified FABP had an apparent molecular mass of 15.0±1.0 kDa as averaged from three different methods, sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), gel filtration and amino acid analysis. The pI value of the protein was determined to be 6.8. Scatchard analysis of fatty acid binding to the purified FABP from malignant prostatic tissue showed a Kd value of 0.53±0.02 μM for arachidonic acid (n=5). The Kd values of FABP purified from benign prostatic tissue were 0.57±0.02 μM for oleic acid and 0.51±0.04 μM for arachidonic acid (n=5). Fatty acid analysis revealed that the level of endogenously bound arachidonic acid was about 2.5-fold higher in FABP from malignant than from benign tissue. In addition, both malignant and benign tissues contained the same concentration of FABP. The concentrations of FABP in malignant and benign tissues were 19.2±1.8 and 21.4±2.1 μg per mg of total cytosolic protein, respectively. Characterization based on amino acid composition, isoelectric point and fluorescence with dansyl undecanoic acid suggests that the FABP may not be of the heart type, but is rather more closely related to the liver type. As malignant prostatic tissue produces more PGE2 compared to benign tissue, our data suggest that FABP may help enhancing the synthesis of the prostaglandin in malignant tissue by facilitating arachidonic acid transport.
Similar content being viewed by others
Abbreviations
- Dansyl undecanoic acid:
-
11-(dansylamino)undecanoic acid
- DTT:
-
dithiothreitok
- EDTA:
-
ethylenediaminetetraacetic acid
- EFA:
-
essential fatty acid
- FABP:
-
fatty acid binding protein(s)
- FPLC:
-
fast protein liquid chromatography
- 15-HPETE:
-
15-hydroperoxyeicosatetraenoic acid
- 5-HPETE:
-
5-hydroperoxyeicosatetraenoic acd
- 5-HETE:
-
5-hydroxyeicosatetraenoic acid
- PITC:
-
phenylisothiocyanate
- PMSF:
-
phenylmethylsulfonyl fluoride
- PGE1 :
-
prostaglandin E1
- PGE2 :
-
prostaglandin E2
- PGD2 :
-
prostaglandin D2
- PGA1 :
-
prostaglandin A1
- PGA2 :
-
prostaglandin A2
- Δ12-PGJ2 :
-
9-deoxyΔ9,12-13,14-dihydro-prostaglandin D2
- SDS-PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
References
Chaudry, A.A., and Dutta-Roy, A.K. (1992)Biochem. Soc. Trans. 20, 117S.
Veerkamp, J.H., Peeters, R.A., and Maatman, R.G.H.J. (1991)Biochim. Biophys. Acta 1081, 1–24.
Kaikaus, R.M., Bass, N.M., and Ockner, R.K. (1990)Experientia 46, 617–630.
Bassuk, J.A., Tsichilis, P.N., and Sorof, S. (1987)Proc. Natl. Acad. Sci. USA 84, 7547–7551.
Dutta-Roy, A.K., Gopalswamy, N., and Trulzsch, D.V. (1987)Eur. J. Biochem. 162, 615–619.
Raza, H., Pongubala, J.R., and Sorof, S. (1989)Biochem. Biophys. Res. Commun. 161, 448–455.
Khan, S.H., and Sorof, S. (1990)Proc. Natl. Acad. Sci. USA 87, 9400–9405.
Narumiya, S., Ohno, K., Fukushima, M., and Fujiwara, M. (1987)J. Pharmacol. Exptl. Ther. 242, 306–311.
Choi, A.M.K., Fargnoli, J., Carlson, S.G., and Holbrook, N.J. (1992)Exptl. Cell Research, 199, 85–89.
Chaudry, A.A., McClinton, S., Moffat, L.F.F., and Wahle, K.W.J. (1991)British J. Cancer 64, 1157–1161.
Dargo, J.R., Rohner, Jr., T.J., and Demers, L.M. (1985)Anticancer Res. 5, 393–396.
Karmali, R.A. (1987)Preventive Medicine.16, 493–502.
Rolland, P.H., Martin, P.M., Jacquemier, J., Rolland, A.M., and Toga, A. (1980)J. Natl. Cancer Inst. 64, 1061–1070.
Fuji, S., Kawaguchi, H., and Yasuda, H. (1987)Hypertension 10, 93–99.
Bradford, M.M. (1976)Anal. Biochem. 72, 248–254.
Glatz, J.F.C., Janssen, A.M., Baerwaldt, C.C.F., and Veerkamp, J.H. (1985)Biochim. Biophys. Acta 837, 57–66.
Scott, K., Cannell, G.R., and Zerner, B. (1975)Anal. Biochem. 69, 474–484.
Vork, M.M., Glatz, J.F.C., Surtel, D.A.M., and Van der Vusse, G.J. (1990)Mol. Cell. Biochem. 98, 111–117.
Folch, L., Lees, M., and Sloane-Stanely, G.H. (1957)J. Biol. Chem. 226, 497–509.
Dutta-Roy, A.K., deMarco, A.C., Raha, S.K., Shay, J., Garvey, M.J., and Horrobin, D.F. (1990)Mol. Cell. Biochem. 98, 177–182.
Dutta-Roy, A.K., Trinh, M.V., Sullivan, T.F., and Trulszch, D.V. (1988)J. Nutr. 118, 1116–1119.
Wilkinson, T.C.I., and Wilton, D.C. (1986)Biochem. J. 238, 419–422.
Peeters, R.A., In't Goren, M.A.P.M., De Moel, M.P., Van Moerkerk, H.T.B., and Veerkamp, J.H. (1989)Int. J. Biochem. 21, 407–418.
Paulussen, R.J.A., Van der Logt, C.R.E., and Veerkamp, J.H. (1988)Arch. Biochem. Biophys. 264, 533–545.
Sweetser, D.A., Birkenmeir, E.H., Klisak, I.J., Zollman, S., Sparkes, R.S., Mohandas, T., Lusis, A.J., and Gordon, J.J. (1987)J. Biol. Chem. 262, 16060–16071.
Haunerland, N., Jagschies, G., Schulenberg, H., and Spener, F. (1984)Hoppe-Seyler's Z. Physiol. Chem. 365, 365–376.
Offner, G.D., Troxler, R.F., and Brecher, P. (1986)J. Biol. Chem. 261, 5584–5589.
Sheridan, M., Wilkinson, T.C.I., and Wilton, D.C. (1987)Biochem. J. 242, 919–922.
Grahams, S., Haughey, B., Marshall, J., Prioore, R., Byers, T., Rzepka, T., Melting, C., and Pontes, J.E., (1983)J. Natl. Cancer Inst. 70, 687–692.
Neoptolemos, J.P., Husband, D., Imray, C., Rowley, S., and Lowson, N. (1991)Gut 32, 278–282.
Chaudry, A.A., Wahle, K.W.J., McClinton, S., and Moffat, L.F.F. (1991) 2nd International Conference on Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation and Radiation Injury, p. 248, Berlin.
Author information
Authors and Affiliations
About this article
Cite this article
Chaudry, A.A., Dutta-Roy, A.K. Purification and characterization of a fatty acid binding protein from human prostatic tissue. Lipids 28, 383–388 (1993). https://doi.org/10.1007/BF02535934
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02535934