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A single-step method for determination of the specific radioactivity of lipids

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Lipids

Abstract

The use of a liquid scintillation counter to measure both the mass and the radioactivity content of charred14C-labeled lipid bands from thin layer chromatoplates has been evaluated. Following lipid mass determination from a measurement of the external standard channels ratio, a suitable choice of counting parameters enabled a reproducible and efficient14C count to be obtained over virtually the whole range of lipid concentrations tested. Although the charring procedure resulted in some loss of radioactivity, the efficiency of counting remained high enough for accurate dpm measurements.

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References

  1. Noble, R.C., J.H. Shand, and I.G. West, Lab. Pract. 28:393 (1979).

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  2. Shand, J.H., and R.C. Noble, Anal. Biochem. 101: 427 (1980).

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Authors and Affiliations

  1. Hannah Research Institute, AYR, KA6 5HL, Scotland

    R. C. Noble & J. H. Shand

Authors
  1. R. C. Noble
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  2. J. H. Shand
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Noble, R.C., Shand, J.H. A single-step method for determination of the specific radioactivity of lipids. Lipids 15, 269–271 (1980). https://doi.org/10.1007/BF02535838

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  • DOI: https://doi.org/10.1007/BF02535838

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