Abstract
Gas liquid chromatographic analysis of diacylglycerols (DGs) and monoacylglycrols (MGs) prepared by Grignard degradation of the triacylglycerols of plant and fish oils was examined as acetate and trimethylsilyl (TMS) ether derivatives on several polar cyanosiloxanes. Satisfactory separations of mixtures of DGs or MGs from simple plant oils based on carbon number and degree of unsaturation, as either acetates or TMS ethers, were obtained on SILAR 10C. In addition, the TMS ethers were effectively separated on the basis of the positional distribution of fatty acid in the acylglycerol molecule, but the acetates of the positional isomers overlapped. The equivalent chain lengths (ECLs) of DGs with 28–42 total acyl carbons and 0–6 double bonds, and MGs with 14–22 acyl carbons and 0–6 double bonds, are presented for both derivatives. The influence of column temperature on the ECLs is discussed.
Similar content being viewed by others
References
Takagi, T., Y. Itabashi, T. Ota, and K. Hayashi, Lipids 11:354 (1976).
Takagi, T., and Y. Itabashi, J. Chromatogr. Sci. 15:121 (1977).
Takagi, T., A. Sakai, Y. Itabashi, and K. Hayashi, Lipids 12:228 (1977).
Takagi, T., A. Sakai, K. Hayashi, and Y. Itabashi, J. Chromatogr. Sci. 17:212 (1979).
Takagi, T., and Y. Itabashi, Lipids 12:1062 (1977).
Kuksis, A., Fette Seifen Anstrichm. 73:332 (1971).
Kuksis, A., in “Lipid Chromatographic Analysis”, 2nd Edition, Vol. 1, Edited by G.V. Marinetti, Marcel Dekker, New York and Basel, 1976, p. 215.
Myher, J.J., in “Handbook of Lipid Research”, Vol. 1, Edited by A. Kuksis, Plenum Press, New York, 1978, p. 123.
Myher, J.J., and A. Kuksis, J. Chromatogr. Sci. 13:138 (1975).
Myher, J.J., L. Marai, A. Kuksis, and D. Kritchevsky, Lipids 12:775 (1977).
Myher, J.J., and A. Kuksis, Lipids 9:382 (1974).
Wood, R.D., P.K. Raju, and R. Reiser, J. Am. Oil Chem. Soc. 42:161 (1965).
Dyer, J.J., and W.E. Klopfenstein, Lipids 12:889 (1977).
Yurkowski, M., and H. Brockerhoff, Biochim. Biophys. Acta. 125:55 (1966).
Kuksis, A., J. Chromatogr. Sci. 10:53 (1972).
Miwa, T.K., K.L. Mikolajizak, F.R. Earle, and L.A. Wolff, Anal. Chem. 32:1739 (1960).
Nelson, G.J., Lipids 9:254 (1974).
Kuksis, A., M.J. McCarthy, and J.M.R. Beveridge, J. Am. Oil. Chem. Soc. 41:201 (1964).
Itabashi, Y., and T. Takagi, Yukagaku 27:219 (1978).
Ettre, L.S., J. Gas Chromatogr. 1:36 (1963).
Watts, R., and R. Dils, J. Lipid Res. 10:33 (1969).
Author information
Authors and Affiliations
About this article
Cite this article
Itabashi, Y., Takagi, T. Gas chromatographic separations of di- and monoacylglycerols based on the degree of unsaturation and positional placement of acyl groups. Lipids 15, 205–215 (1980). https://doi.org/10.1007/BF02535829
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02535829