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Acid triacylglycerol lipase from bovine thyroid gland

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Lipids

Abstract

An acid lipase has been detected in bovine thyroid tissue using triolein as a substrate. The activity, probably associated with the lysosomes, displays a rather broad pH-optimum in the pH 4 to pH 6.5 range. The lipase activity can be partially purified by cosedimentation with lysosomes followed by solubilization through detergent and chromatography on Sephadex G-200 and carboxymethyl cellulose. The elution profile on Sephadex G-200 shows one peak (moleculare weight 67,000±2,000). In the final CM-cellulose step, two lipase peaks (lipase LA and lipase LB) are found. Sulhydryl reagents (iodoacetate, iodoacetamide, and N-ethylmaleimide) as well as mercuric ions markedly reduce both enzyme activities. Calcium ions, EDTA, and heparin have no effect. Sodium fluoride and diisopropylfluorophosphate are only slightly inhibitory. Sodium chloride causes a slight increase in both lipase activities. Anionic phospholipids such as cardiolipin and phosphatidylserine are not essential for enzyme activity.

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References

  1. Brockerhoff, H., and R.G. Jensen, in “Lipolytic Enzymes”, Academic Press, New York, 1974, p. 25.

    Google Scholar 

  2. Burke, G., Prostaglandins 3:291 (1973).

    Article  PubMed  CAS  Google Scholar 

  3. Haye, B., S. Champion, and C. Jacquemin, FEBS Lett. 41:89 (1974).

    Article  PubMed  CAS  Google Scholar 

  4. Haye, B., and C. Jacquemin, Biochim. Biophys. Acta 487:231 (1977).

    PubMed  CAS  Google Scholar 

  5. Hilderson, H.J., A. Lagrou, and W. Dierick, Biochim. Biophys. Acta 337:385 (1974).

    PubMed  CAS  Google Scholar 

  6. Sims, N.R., and P.R. Carnegie, Anal. Biochem. 65:578 (1975).

    Article  PubMed  CAS  Google Scholar 

  7. Martin, J.J., G. Van Dessel, A. Lagrou, A.M. De Barsy, and W. Dierick, J. Neurol. Sci. 21:215 (1974).

    Article  Google Scholar 

  8. Mahadevan, S., C.J. Dillard, and A.L. Tappel, Anal. Biochem. 27:387 (1969).

    Article  PubMed  CAS  Google Scholar 

  9. Bligh, E.G., and W.J. Dyer, Can. J. Biochem. Physiol. 37:911 (1959).

    PubMed  CAS  Google Scholar 

  10. Hilderson, H.J.J., M.J.S. De Wolf, A.R. Lagrou, and W.S.H. Dierick, Biochem. J. 152:601 (1975).

    PubMed  CAS  Google Scholar 

  11. Wurtman, R.J., and J. Axelrod, Biochem. Pharmacol. 12:1439 (1963).

    Article  PubMed  CAS  Google Scholar 

  12. Fiers, W., and J. Stockx, Naturwissenschaften 44:115 (1957).

    Article  CAS  Google Scholar 

  13. De Wolf, M., A. Lagrou, H.J. Hilderson, G. Van Dessel, and W. Dierick, Biochem. J. (In press).

  14. Hilderson, H.J., J. Stockx, and W. Dierick, Arch. Int. Physiol. Biochim. 78:509 (1970).

    PubMed  CAS  Google Scholar 

  15. De Wolf, M., H.J. Hilderson, and W. Dierick, in “Methodological Surveys in Biochemistry”, Vol. 6, Edited by E. Reid, Ellis Horwood Ltd., Chichester, UK, 1977, p. 251.

    Google Scholar 

  16. Teng, M.H., and A. Kaplan, J. Biol. Chem. 249:1064 (1974).

    PubMed  CAS  Google Scholar 

  17. Ledford, J.H., and P. Alaupovic, Biochim. Biophys. Acta 398:132 (1975).

    PubMed  CAS  Google Scholar 

  18. Rindler-Ludwig, R., W. Patsch, S. Sailer, and H. Braunsteiner, Biochim. Biophys. Acta 488:294 (1977).

    PubMed  CAS  Google Scholar 

  19. Claycomb, W.C., and G.S. Kilsheimer, J. Biol. Chem. 246:7139 (1971).

    PubMed  CAS  Google Scholar 

  20. Mattson, F.H., and L.W. Beck, J. Biol. Chem. 219:735 (1956).

    PubMed  CAS  Google Scholar 

  21. Desnuelle, P., Biochimie 53:841 (1971).

    PubMed  CAS  Google Scholar 

  22. Maylié, M.F., M. Charles, and P. Desnuelle, Biochim. Biophys. Acta 276:162 (1972).

    PubMed  Google Scholar 

  23. Mahadevan, S., and A.L. Tappel, J. Biol. Chem. 243:2849 (1968).

    PubMed  CAS  Google Scholar 

  24. Mann, J.T., and S.B. Tove, J. Biol. Chem. 241:3595 (1966).

    PubMed  CAS  Google Scholar 

  25. Lagrou, A., W. Dierick, A. Christophe, and G. Verdonk, Lipids 9:870 (1974).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. RUCA-Laboratory for Human Biochemistry, University of Antwerp, B2020, Antwerp, Belgium

    M. De Wolf, A. Lagrou, H. J. Hilderson & W. Dierick

Authors
  1. M. De Wolf
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  2. A. Lagrou
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  3. H. J. Hilderson
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  4. W. Dierick
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De Wolf, M., Lagrou, A., Hilderson, H.J. et al. Acid triacylglycerol lipase from bovine thyroid gland. Lipids 13, 598–604 (1978). https://doi.org/10.1007/BF02535822

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  • DOI: https://doi.org/10.1007/BF02535822

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