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Isolation and characterization of the monounsaturated long chain fatty acids ofMycobacterium tuberculosis

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Lipids

Abstract

The positions of double bond in the monounsaturated C15−C32 fatty acids ofMycobacterium tuberculosis H37Ra were established by gas chromatography/mass spectrometry of the ozonized esters and their pyrrolidide derivatives. The monounsaturated C15−C21 fatty acids had the double bond primarily at the Δ9 position while the monounsaturated longer chain fatty acids (C22−C32) had the double bond in several positions. Many of the latter acids, especially the odd-numbered series, were very complex isomeric mixtures. Quantitation showed the most abundant even-numbered long chain fatty acid isomers to be as follow: C22, Δ4; C24, Δ5; C26, Δ7 and Δ9; C28, Δ9; C30, Δ11 and Δ13; C32, Δ13 and Δ15.

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Authors and Affiliations

  1. Tuberculosis Research Laboratory, Williams S. Middleton Memorial Veterans Administration Hospital, 53706, Madison, Wilsconsin

    Kuni Takayama & Nilofer Quereshi

  2. The Institute for Enzyme Research, University of Wisconsin, 53706, Madison, Wilsconsin

    Kuni Takayama & Nilofer Quereshi

  3. Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, 53706, Madison, Wisconsin

    Heinrich K. Schnoes

Authors
  1. Kuni Takayama
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  2. Nilofer Quereshi
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  3. Heinrich K. Schnoes
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Takayama, K., Quereshi, N. & Schnoes, H.K. Isolation and characterization of the monounsaturated long chain fatty acids ofMycobacterium tuberculosis . Lipids 13, 575–579 (1978). https://doi.org/10.1007/BF02535818

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  • DOI: https://doi.org/10.1007/BF02535818

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