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(3H/14C)Beta-methylheptadecanoic acid subcellular distribution and lipid incorporation in mouse heart

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Lipids

Abstract

Beta-methylfatty acids are transported into myocardial cells as other fatty acids would be, but are incapable, for example, of undergoing complete catabolism. Our previous studies have established the structure-location relationship and the imaging characteristics of these analogs. In the present study in mouse myocardium, microautoradiography and electron microscopy were used to show the distribution of [7,8-3H] beta-methylheptadecanoic acid ([3H]BMHA) in mitochondria and lipid droplets. Thin-layer chromatography demonstrated the presence of [1-14C] beta-methylheptadecanoic acid ([14C]BMHA) and its metabolites in various lipid pools. These studies complement our earlier findings which showed that similarities exist in the initial metabolic fate of BMHA and physiological fatty acids.

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Abbreviations

NEFA:

Nonesterified fatty acids

BMHA:

beta-methylheptadecanoic acid

EM:

electron microscopy

References

  1. Zierler, K.L. (1976)Circ. Res. 38, 459–463.

    PubMed  CAS  Google Scholar 

  2. Raichle, M.E., Welch, M.J., Grubb, R.L. Jr., Higgins, C.S., Ter-Pogossian, M.M., and Larson, K.B. (1978)Science 199, 986–987.

    Article  PubMed  CAS  Google Scholar 

  3. Klein, M.S., Goldstein, R.A., Welch, M.J., and Sobel, B.E. (1979)Am. J. Physiol. 237, H51-H58.

    PubMed  CAS  Google Scholar 

  4. Rosamond, T.L., Abendschein, D.R., Sobel, B.E., Bergmann, S.R., and Fox, K.A.A. (1987)J. Nucl. Med. 28, 1322–1329.

    PubMed  CAS  Google Scholar 

  5. Reivich, M., Kuhl, D., Wolf, A., Greenberg, J., Phelps, M., Ido, T., Casella, V., Fowler, J., Hoffman, E., Alavi, A., Som, P., and Sokoloff, L. (1979)Circ. Res. 44, 127–137.

    PubMed  CAS  Google Scholar 

  6. Livni, E., Elmaleh, D.R., Levy, S., Brownell, G.L., and Srauss, H.W. (1982)J. Nucl. Med. 23, 169–175.

    PubMed  CAS  Google Scholar 

  7. Elmaleh, D.R., Livni, E., Levy, S., Varnum, D., Strauss, H.W., and Brownell, G.L. (1983)Int. J. Nucl. Med. Biol. 10, 181–187.

    Article  PubMed  CAS  Google Scholar 

  8. Eddy, E.M., and Ito, S. (1971)J. Cell. Biol. 49, 90–108.

    Article  PubMed  CAS  Google Scholar 

  9. Folch, J., Lees, M., and Stanley, G.H.S. (1957)J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  10. Harris, P., Gloster, J.A., and Ward, B.J. (1980)Arch. Mal. Coeur 73, 593–598.

    PubMed  CAS  Google Scholar 

  11. Knapp, F.F. Jr., Ambrose, K.R., and Goodman, M.M. (1986)Eur. J. Nucl. Med. 12, S39-S44.

    Article  PubMed  CAS  Google Scholar 

  12. Abendschein, D.R., Fox, K.A.A., Ambos, H.D., Sobel, B.E., and Bergmann, S.R. (1987)Nucl. Med. Biol., 14, 579–585.

    CAS  Google Scholar 

  13. Yonekura, Y., Brill, A.B., Som, P., Yamamoto, K., Srivastava, S.C., Iwai, J., Elmaleh, D.R., Livni, E., Strauss, H.W., Goodman, M.M., and Knapp, F.F. Jr. (1985)Science 227, 1494–1496.

    Article  PubMed  CAS  Google Scholar 

  14. de Landsheere, C., Wilson, R., Shea, M., Pike, V., Elmaleh, D., Livni, E., Jones, T., Maseri, A., and Selwyn, A. (1983)Circulation 68, III-139 (Abstract).

    Google Scholar 

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Authors and Affiliations

  1. Massachusetts General Hospital, Department of Radiology, Division of Radiological Sciences and Technology, 02114, Boston, MA

    Eli Livni & David R. Elmaleh

  2. Department of Anatomy and Cellular Biology, Harvard Medical School, 02115, Boston, MA

    Susumu Ito

  3. Department of Radiology, Harvard Medical School, 02115, Boston, MA

    Amin I. Kassis

Authors
  1. Eli Livni
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  2. Susumu Ito
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  3. Amin I. Kassis
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  4. David R. Elmaleh
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Livni, E., Ito, S., Kassis, A.I. et al. (3H/14C)Beta-methylheptadecanoic acid subcellular distribution and lipid incorporation in mouse heart. Lipids 25, 238–240 (1990). https://doi.org/10.1007/BF02535755

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  • DOI: https://doi.org/10.1007/BF02535755

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