Abstract
A previously uncharacterized fatty acid found inCorynebacterium sepedonicum was analyzed by gas chromatography-mass spectrometry and infrared spectrometry and determined to be 12-methyl-cis-4-tetradecenoic acid. This fatty acid represents nearly 6% of the total fatty acids ofC. sepedonicum, but it is present at significantly lower levels in otherCorynebacterium species. Therefore, 12-methyl-cis-4-tetradecenoic acid provides a useful marker in distinguishingC. sepedonicum from other microorganisms.
Similar content being viewed by others
Abbreviations
- ECL:
-
equivalent chain length
- GC-MS:
-
gas chromatography-mass spectrometry
References
Moss, C.W. (1981)J. Chromatogr. 203, 337–347.
Dees, S.B., Powell, J., Moss, C.W., Hollis, D.G., and Weaver, R.E. (1981)J. Clin. Microbiol. 14, 612–616.
De Boer, S.H., and Sasser, M. (1986)Can. J. Microbiol. 32, 796–800.
Gudmestad, N.C., Henningson, P.J., and Bugbee, W.M. (1988)Can. J. Microbiol., in press.
Bugbee, W.M., Gudmestad, N.C., Secor, G.A., and Nolte, P. (1987)Phytopathology 77, 765–770.
De Boer, S.H., and Weiczorek, A. (1984)Phytopathology 74, 1431–1434.
Suzuki, I.K., and Komagata, K. (1983)Int. J. Syst. Bacteriol. 33, 188–200.
Miller, L., and Berger, T. (1985) Hewlett-Packard Gas Chromatography Application Note No. 228-41.
Andersson, B.A., and Holman, R.T. (1974)Lipids 9, 185–190.
Kaneda, T. (1977)Bacteriol. Rev. 41, 391–418.
Durham, D.R. (1978)Int. J. Syst. Bacteriol. 28, 223–228.
Bousfield, I.J., Smith, G.L., Dando, T.R., and Hobbs, G. (1983)J. Gen. Microbiol. 129, 375–394.
Author information
Authors and Affiliations
Additional information
Published with the approval of the Director of the North Dakota Agricultural Experiment Station as Journal Article #1694.
About this article
Cite this article
Henningson, P.J., Vick, B.A., Bugbee, W.M. et al. Characterization of 12-methyl-cis-4-tetradecenoic acid fromCorynebacterium sepedonicum . Lipids 23, 1086–1088 (1988). https://doi.org/10.1007/BF02535657
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02535657