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A diet containing n−3 and n−6 fatty acids favorably alters the renal phospholipids, eicosanoid synthesis and plasma lipids in nephrotic rats

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Lipids

Abstract

The nephrotic syndrome was induced in rats by intravenous adriamycin (3 mg/kg). The rats were then divided into four groups which, for six weeks, were pair-fed diets containing beef tallow (BT), fish oil (FO), a source of n−3 fatty acids, evening primrose oil (EPO), a source of n−6 fatty acids, or a combination of evening primrose oil and fish oil, 75∶25 (EPO:FO). The fat content of the diets was 15%. Significant incorporation of the fatty acids into kidney phospholipids was demonstrated. Diets containing FO, EPO and EPO:FO lowered plasma triglycerides and total cholesterol levels as compared with diets containing BT. Only EPO:FO raised high density lipoprotein (HDL) cholesterol levels, as compared with BT. The combination EPO:FO prevented the tenfold suppression of aortic 6-keto-PGF1α caused by FO. These changes in plasma lipids and eicosanoid production are potentially antiatherogenic and may prevent glomerular sclerosis. The combination of EPO and FO, containing n−6 and n−3 fatty acids may offer advantages over either family of fatty acids in this model of nephrotic syndrome.

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Abbreviations

BSA:

bovine serum albumin

BT:

beef tallow

EBSS:

Earls Balanced Salt Solution

EPO:

evening primrose oil

FO:

fish oil

HDL:

high density lipoprotein

PG:

prostaglandin

PUFA:

polyunsaturated fatty acid

SBO:

soybean oil

TXA2 :

thromboxane A2

(V)LDL:

(very) low density lipoprotein

GC:

gas chromatography

HPLC:

high-performance liquid chromatography

PAS:

periodic acid-Schiff

RIA:

radioimmunoassay

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Authors and Affiliations

  1. Departments of Internal Medicine and Pathology, University of Cincinnati Medical Center, 45267-O585, Cincinnati, OH

    U. O. Barcelli, D. C. Beach, B. Thompson, M. Weiss & V. E. Pollak

Authors
  1. U. O. Barcelli
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  2. D. C. Beach
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  3. B. Thompson
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  4. M. Weiss
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  5. V. E. Pollak
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Barcelli, U.O., Beach, D.C., Thompson, B. et al. A diet containing n−3 and n−6 fatty acids favorably alters the renal phospholipids, eicosanoid synthesis and plasma lipids in nephrotic rats. Lipids 23, 1059–1063 (1988). https://doi.org/10.1007/BF02535652

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  • DOI: https://doi.org/10.1007/BF02535652

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