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The effect of feeding fish oils, vegetable oils and clofibrate on the ketogenesis from long chain fatty acids in hepatocytes

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Lipids

Abstract

Groups of rats were fed diets containing 25% fish oil (FO), 25% soybean oil, 25% partially hydrogenated fish oil (PHFO), 25% partially hydrogenated soybean oil (PHSO), 25% partially hydrogenated coconut oil or 0.3% clofibrate for 3 wk. After the animals were fasted for 24 hr, hepatocytes were isolated and ketogenesis from added palmitate, linoleatecis andtrans, arachidonate and docosahexaenoate was measured. Ketogenesis after oil feeding was significantly stimulated (two-to threefold) only in cells from the PHFO-and PHSO-fed rats. The stimulation was most apparent with the long chain unsaturated fatty acids as substrates. These fatty acids were relatively poor ketone body precursors in control hepatocytes. Essential fatty acid deficiency did not seem to be the reason for this stimulation. Clofibrate also stimulated ketogenesis significantly (1.5- to 3-fold). The degree of stimulation increased with chain length and degree of unsaturation of the substrate. The activity of the enzyme 2,4-dienoyl-CoA reductase was also studied in the same groups. Its activity was stimulated about fourfold in the clofibrate-treated rats and to a lesser extent by the PHFO, PHSO and FO diets. The activity showed no correlation with the content of unsaturated fatty acids in the diet or their oxidation in isolated hepatocytes. The 2,4-dienoyl-CoA reductase, therefore, does not seem to be a regulatory enzyme in the metabolism of dietary polyunsaturated fatty acids. It is concluded that an induction of the peroxisomal β-oxidation system most likely is involved in the reported increases in ketogenesis from very long chain polyunsaturated fatty acids.

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References

  1. Lazarow, P.B., and de Duve, C. (1976)Proc. Natl. Acad. Sci. USA 73, 2043–2046.

    Article  PubMed  CAS  Google Scholar 

  2. Lazarow, P.B. (1977)Science 197, 580–581.

    Article  PubMed  CAS  Google Scholar 

  3. Neat, C.E., Thomassen, M.S., and Osmundsen, H. (1981)Biochem. J. 196, 149–159.

    PubMed  CAS  Google Scholar 

  4. Christiansen, R.Z., Osmundsen, H., Borrebaek, B., and Bremer, J. (1978)Lipids 13, 487–491.

    Article  PubMed  CAS  Google Scholar 

  5. Christiansen, R.Z., Christiansen, E.N., and Bremer, J. (1979)Biochim. Biophys. Acta 573, 417–429.

    PubMed  CAS  Google Scholar 

  6. Borrebaek, B., Osmundsen, H., Christiansen, E.N., and Bremer, J. (1980)FEBS Lett. 121, 23–24.

    Article  PubMed  CAS  Google Scholar 

  7. Borrebaek, B., Osmundsen, H., and Bremer, J. (1980)Biochem. Biophys. Res. Commun. 93, 1173–1180.

    Article  PubMed  CAS  Google Scholar 

  8. Kunau, W.-H., and Dommes, P. (1978)Eur. J. Biochem. 91, 533–544.

    Article  PubMed  CAS  Google Scholar 

  9. Osmundsen, H., Cervenka, J., and Bremer, J. (1982)Biochem. J. 208, 749–757.

    PubMed  CAS  Google Scholar 

  10. Hiltunen, J.K., and Davies, E.J. (1980)Biochem. J. 194, 427–432.

    Google Scholar 

  11. Hiltunen, J.K., Osmundsen, H., and Bremer, J. (1983)Biochim. Biophys. Acta 752, 223–232.

    PubMed  CAS  Google Scholar 

  12. Thomassen, M.S., Christiansen, E.N., and Norum, K.R. (1982)Biochem. J. 206, 195–202.

    PubMed  CAS  Google Scholar 

  13. Thomassen, M.S., Strøm, E., Christiansen, E.N., and Norum, K.R. (1979)Lipids 14, 58–65.

    Article  PubMed  CAS  Google Scholar 

  14. Ontko, J.A. (1972)J. Biol. Chem. 247 1788–1800.

    PubMed  CAS  Google Scholar 

  15. Guynn, R.W., Veloso, D., and Veech, R.L. (1972)J. Biol. Chem. 247, 7325–7331.

    PubMed  CAS  Google Scholar 

  16. Seglen, P.O. (1973)Exp. Cell Res. 82, 391–398.

    Article  PubMed  CAS  Google Scholar 

  17. Christiansen, R.Z. (1977)Biochim. Biophys. Acta 488, 249–262.

    PubMed  CAS  Google Scholar 

  18. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  19. Williamson, D.H., and Mellanby, J. (1974) inMethods of Enzymatic Analyses, 2nd edn., pp. 1836–1843, Academic Press, New York.

    Google Scholar 

  20. Whitaker, J.R., and Granum, P.E. (1980)Anal. Biochem. 109, 156–159.

    Article  PubMed  CAS  Google Scholar 

  21. Schulz, H. (1974)J. Biol. Chem. 249, 2704–2709.

    PubMed  CAS  Google Scholar 

  22. Chase, J.F.A., and Tubbs, P.K. (1966)Biochem. J. 99, 32–40.

    PubMed  CAS  Google Scholar 

  23. LaNoue, K., Niklas, W.J., and Williamson, J.R. (1970)J. Biol. Chem. 245, 102–111.

    PubMed  CAS  Google Scholar 

  24. Stakkestad, J.A., and Bremer, J. (1983)Biochim. Biophys. Acta 750, 244–252.

    PubMed  CAS  Google Scholar 

  25. Christiansen, R.Z. (1978)Biochim. Biophys. Acta 530, 314–324.

    PubMed  CAS  Google Scholar 

  26. Williamson, D.H., Bates, M.W., and Krebs, H.A. (1968)Biochem. J. 108, 353–361.

    PubMed  CAS  Google Scholar 

  27. Reeves, J.B. III, and Weihrauch, J.L. (Principal Investigators) (Revised 1979)Composition of Foods, Fats and Oils—Raw-Processed-Prepared, Agriculture Handbook No. 8-4, United States Department of Agriculture Science and Education Administration.

  28. Wong, S.H., Nestel, P.J., Trimble, R.P., Storer, G.B., Illman, R.J., and Topping, D.L. (1984)Biochim. Biophys. Acta 792, 103–109.

    PubMed  CAS  Google Scholar 

  29. Hertz, R., Arnon, J., and Bar-Tana, J. (1985)Biochim. Biophys. Acta 836, 192–200.

    PubMed  CAS  Google Scholar 

  30. Ishii, H., Fukumori, N., Horie, S., and Suga, T. (1980)Biochim. Biophys. Acta 617, 1–11.

    PubMed  CAS  Google Scholar 

  31. Mizugaki, M., Nishimaki, T., Yamamoto, H., Sagi, M., and Yamanaka, H. (1982)J. Biochem. 92, 2051–2054.

    PubMed  CAS  Google Scholar 

  32. Dommes, V., Baumgart, C., and Kunau, W.-H. (1981)J. Biol. Chem. 256, 8259–8262.

    PubMed  CAS  Google Scholar 

  33. Alexson, S.E.H., and Cannon, B. (1984)Biochim. Biophys. Acta 796, 1–10.

    PubMed  CAS  Google Scholar 

  34. Nilsson, A., Thomassen, M.S., and Christiansen, E. (1984)Lipids 19, 187–194.

    Article  PubMed  CAS  Google Scholar 

  35. Berge, R.K., and Aarsland, A. (1985)Biochim. Biophys. Acta 837, 141–151.

    PubMed  CAS  Google Scholar 

  36. Hagve, T.-A., and Christophersen, B.O. (1986)Biochim. Biophys. Acta 875, 165–173.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute for Nutrition Research, University of Oslo, Blindern, P.O. Box 1112, 0317, Oslo 3, Norway

    Erling N. Christiansen

  2. Institute of Medical Biochemistry, University of Oslo, Blindern, P.O. Box 1112, 0317, Oslo 3, Norway

    Steinar Bergseth & Jon Bremer

Authors
  1. Steinar Bergseth
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  2. Erling N. Christiansen
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  3. Jon Bremer
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Bergseth, S., Christiansen, E.N. & Bremer, J. The effect of feeding fish oils, vegetable oils and clofibrate on the ketogenesis from long chain fatty acids in hepatocytes. Lipids 21, 508–514 (1986). https://doi.org/10.1007/BF02535638

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  • DOI: https://doi.org/10.1007/BF02535638

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